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作者：Cottrell, Oliver; Rowntree, Andrew; Chopra, Kunal; Mackellar, Eleanor; Noble, Benjamin; Dixon, Hannah L.; Healy, Ciara; Clarke, Robert B.; Papalopulu, Nancy
作者单位：University of Manchester; University of London; Queen Mary University London; University of Manchester
摘要：Long-term recurrence in breast cancer is driven by reactivation of dormant disseminated tumor cells (DTCs) and remains a major clinical challenge, particularly in estrogen receptor-positive (ER+) tumors. This process is underpinned by regulation of the cell cycle machinery that controls quiescence maintenance and exit. HES1, a Notch pathway transcription factor, regulates key cell cycle genes and has been shown to demonstrate protein expression oscillations. Here, we sought to establish whethe...
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作者：Mizuno, Sonoko; Uemura, Tadashi; Kondo, Takefumi
作者单位：RIKEN; Kyoto University; Kyoto University; Kyoto University
摘要：During development, cells sequentially acquire specific fates through temporally ordered regulatory systems. To ensure the harmonious progression, each system must be activated and subsequently inactivated at the appropriate time. In this study, we show that the duration of fate induction is controlled by the transient expression of polished rice (pri), a gene encoding micropeptides, during Drosophila tracheal development. pri is transiently expressed in prospective tracheal placodes and prece...
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作者：Peeples, Lynne
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作者：German, Alexander; Akdas, Enes Yagiz; Flugel-Koch, Cassandra; Erterek, Ezgi; Frischknecht, Renato; Fejtova, Anna; Winkler, Jurgen; Alzheimer, Christian; Zheng, Fang
作者单位：University of Erlangen Nuremberg; University of Erlangen Nuremberg; Technical University of Munich; University of Erlangen Nuremberg; University of Erlangen Nuremberg; University of Erlangen Nuremberg
摘要：Cryopreserving the adult brain is challenging due to damage from ice formation, and traditional freezing methods fail to maintain neural architecture and function. Vitrification offers a promising alternative but has not been surveyed in the brain. Here, we demonstrate short-term recovery of the adult murine hippocampus after vitrification of brain slices and of the whole brain in situ. Key features of the hippocampus are preserved, including structural integrity, metabolic responsiveness, neu...
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作者：Janovicova, Lubica; Janikova, Monika; Pastorek, Michal; Csizmadia, Eva; Yoder, Cherith; Lesayova, Alexandra; Geyer, Liam; Kyttaris, Vasileios C.; Tsokos, George C.; Celec, Peter; Wegiel, Barbara
作者单位：Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Beth Israel Deaconess Medical Center; Comenius University Bratislava; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Beth Israel Deaconess Medical Center
摘要：Hemolysis is associated with the release of damage-associated molecular patterns, including free heme and extracellular DNA (ecDNA). Using several mouse models of bleeding anemia and hemolysis, we demonstrate a significant increase in plasma ecDNA, independent of neutrophil extracellular trap formation. This ecDNA forms G-quadruplex (G4) structures, which we detected in both mice and patients with systemic lupus erythematosus. Catalytic complexes (DNAzymes) formed by G4 ecDNA and heme drive ox...
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作者：Sanchez-Morales, Lara M.; Beach, Timothy P.; Luzzadder-Beach, Sheryl; Krause, Samantha; Cook, Duncan; Smith, Byron; Lentz, David; Quiroz, Carlos; Pratt, William; Phillips, Lori; Guderjan, Thomas; Hanratty, Colleen; Valdez Jr, Fred
作者单位：New York University; University of Texas System; University of Texas Austin; Texas State University System; Texas State University San Marcos; Australian Catholic University; Australian Catholic University - Brisbane Campus; California State University System; California State Polytechnic University, Humboldt; University System of Ohio; University of Cincinnati; University of Texas System; University of Texas at Tyler; University of Texas System; University of Texas Austin
摘要：Rapid decay of organic artifacts obscures our view of ancient tropical settlements. We present a study of architecture and artifact assemblages from an ancient settlement excavated at the Birds of Paradise (BOP) fields, the largest lidar and field-validated ancient Maya wetland field complex. The site's unique setting preserved architectural Postclassic (CE 1000 to 1500) persistence within tropical wetlands through periods of large-scale societal and climate change. A new Bayesian chronologica...
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作者：Zhang, Zhenqian; Yu, Zhenghao; Xie, Dixiang; Wang, Ju; Li, Jingrong; Lai, Duoduo; Gao, Yaqi; Li, Jiaxin; Ju, Chuanfeng; Zhang, Yuzhou; Liu, Kun - Hsiang; Wang, Cun
作者单位：Northwest A&F University - China; Northwest A&F University - China; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School
摘要：Manganese (Mn) toxicity in acidic or waterlogged soils severely impacts crop productivity. Although high-Mn stress triggers Ca2+ signals that regulate Mn homeostasis, the mechanism generating these signals remains unclear. Here, we show that the cyclic nucleotide-gated channel CNGC11/12 are essential for Mn tolerance, as cngc11/12 mutants exhibited hypersensitivity to Mn and cngc12 mutant showed reduced Ca2+ elevations. The brassinosteroid (BR) receptor BRI1 physically interacted with CNGC12 a...
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作者：Eames, Taryn; Brunskill, Emma; Yamkovenko, Bogdan; Weatherholtz, Kodi; Oreopoulos, Philip
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作者：Qin, Wei; Tagliabue, Alessandro; Hou, Lei; Xu, Min; Bian, Xiaopeng; Moran, Dawn M.; Zhao, Duo; Li, Qian; McIlvin, Matthew R.; Zheng, Yue; Kao, Shuh-Ji; Zhang, Yao; Saito, Mak A.; John, Seth G.; Fu, Fei-Xue; Hutchins, David A.
作者单位：University of Illinois System; University of Illinois Urbana-Champaign; University of Liverpool; University of Oklahoma System; University of Oklahoma - Norman; Xiamen University; Hainan University; University of Southern California; Woods Hole Oceanographic Institution; Xiamen University; University of Southern California
摘要：Ammonia- oxidizing archaea (AOA) are among the most abundant microorganisms in the ocean, playing a fundamental role in the marine nitrogen cycle. Although temperature and trace metal availability each individually influence the growth and activity of marine AOA, there is only a very limited understanding of the interactive effects of these two major factors on AOA in the rapidly changing ocean. Here, we show that the iron requirements of the model marine AOA species Nitrosopumilus maritimus S...
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作者：Zhu, Xinyu; Nakano, Satoko; Rao, Vishwas N.; Miranda, Hector A.; Spurrier, M. Ariel; Skavicus, Samantha; Luo, Zhaochen; Heaton, Nicholas S.; Sastre, Adolfo Garcia -
作者单位：Duke University; Duke University; Duke University
摘要：Sialic acid is an abundant terminal glycan found on the surface of almost all mammalian cells and is utilized by many viruses as an attachment factor and/or bona fide receptor. Altering canonical cellular glycosylation can affect the ability of viruses to initiate infections; however, no systematic analysis of the effects of altering expression of glycan capping enzymes on viral infection rates has been previously performed. We therefore designed an arrayed CRISPR activation screen that target...
