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作者：Niu, Junhua; Yu, Haoze; Liu, Yongqiang; Nie, Lanheng; Nan, Bei; Zhang, Wenxin; Song, Ni; Rong, Shaoqin; Zhou, Dan; Gao, Shan
作者单位：Ocean University of China; Ocean University of China; Yunnan University; Ocean University of China; Chinese Academy of Sciences; Center for Excellence in Molecular Cell Science, CAS; Fudan University
摘要：DNA N6-methyladenine (6mA) is a newly recognized transcription-associated epigenetic mark in eukaryotes. While its methylation pathway has been well established, the identity of eukaryotic 6mA demethylase(s) responsible for its removal and dynamic regulation has remained elusive. Here, we identify and characterize DMT3 (TtALKBH5), an AlkB family dioxygenase in Tetrahymena thermophila, as a 6mA demethylase in ciliates and potentially several other unicellular eukaryotes with abundant 6mA and a ...
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作者：Yanaka, Saeko; Sakae, Yoshitake; Miyanoiri, Yohei; Yamaguchi, Takumi; Isono, Yukiko; Kondo, Sachiko; Iwasaki, Miyuki; Onitsuka, Masayoshi; Yagi, Hirokazu; Kato, Koichi
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作者：Zhang, Xiujuan; Richart, Donovan; McFarlin, Shane; Cheng, Fang; Park, Soo Yeun; Zhang-Chen, Anwen; McFarlane, Richenda; Xiao, Chuan; Yan, Ziying; Qiu, Jianming
作者单位：University of Kansas; University of Kansas Medical Center; University of Alabama System; University of Alabama Birmingham; University of Texas System; University of Texas El Paso
摘要：Recombinant adeno-associated viruses (rAAVs) are widely used for in vivo gene delivery. While KIAA0319L, known as AAV receptor (AAVR), is essential for the transduction of multiserotype AAVs, it is dispensable for AAV4-related (Clade G) AAVs. We conducted a genome-wide CRISPR/Cas9 screen and identified CD164, a type I transmembrane sialomucin, as an essential entry receptor for Clade G AAVs. Ablation of CD164 expression substantially impaired both entry and transduction of Clade G AAVs. CD164-...
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作者：Yi, Junxi; Xie, Fangbai; Moller, Jennifer Q.; Xu, Zhenchuang; Zhao, Shensheng; Diao, Ying; Leakey, Andrew D. B.; Moore, Jeffrey S.; Chen, Yun -Sheng
作者单位：University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign
摘要：This study presents a biocompatible, ultrasound-responsive platform for remotely activating mechanochemical reactions within live plant tissue. Fluorogenic Mechanophore-embedded silica NanoParticles (FMNPs) that are thermally stable were engineered to emit blue fluorescence at 440 nm upon mechanical activation. In Solanum lycopersicum (tomato) leaves, activation was achieved through the synergistic combination of gas vesicles (GVs) and high-frequency focused ultrasound (FUS, 550 kHz), enabling...
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作者：Berger, Bonnie; Eisenberg, David S.
作者单位：Massachusetts Institute of Technology (MIT); University of California System; United States Department of Energy (DOE); University of California Los Angeles; David Geffen School of Medicine at UCLA
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作者：Dai, Dadong; Zhang, Shurong; Hu, Boyan; Zhou, Yayi; Cui, Simeng; Sun, Jia; Zhang, Yali; Wang, Xueyu; Siddique, Shahid; Bo, Dexin; Zhang, Min; Williamson, Valerie M.; Yao, Haozhe; Duan, Xinya; Wu, Wentao; Peng, Donghai; Zheng, Jinshui; Sun, Ming
作者单位：Huazhong Agricultural University; Hubei Hongshan Laboratory; University of California System; University of California Davis; University of California System; University of California Davis; Yunnan Agricultural University
摘要：Understanding the global regulatory mechanisms that control pathogen virulence gene expression is essential for elucidating the molecular basis of pathogenicity. N6-methyladenine (6 mA) plays a crucial role in regulating gene expression in response to various environmental stresses; however, its role in pathogen virulence remains largely unexplored. Here, we report the widespread occurrence of 6 mA across 17 nematode isolates and map its genomic landscape in six notorious agriculturally import...
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作者：Gao, Lin; Cho, Yongjoon; Wang, Rui; Yao, Yao; Zhang, Dayong; Wang, Yifan; Cheng, Yongfa; Wang, Yuyang; Ma, Qing; Duplessis, Isaiah; Chung, Sein; Wilson, Evan; Cho, Kilwon; Wang, Binghao; Li, Lu; Rivnay, Jonathan; Wang, Hong; Yu, Junsheng; Marks, Tobin J.; Facchetti, Antonio
作者单位：Chongqing University of Arts & Sciences; Northwestern University; Northwestern University; Daegu Gyeongbuk Institute of Science & Technology (DGIST); Xidian University; Southeast University - China; Pohang University of Science & Technology (POSTECH); University System of Georgia; Georgia Institute of Technology; Northwestern University
摘要：Organic semiconductors offering efficient mixed ionic-electronic charge transport are key components of organic electrochemical transistors (OECTs) needed for future bioelectronics and other technologies. However, hydrophobic semiconductors typically have limited ion mobility and are unstable in aqueous environments, restricting OECT applications. To address these issues, we report a broadly applicable strategy for high-performance OECTs by blending polymeric semiconductors with a photocrossli...
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作者：Jeltema, Devon; Yang, Kun; Baty, Joshua J.; Araszkiewicz, Antonina; Xing, Cong; Knox, Kennady; Tang, Zhen; Dobbs, Nicole; Yan, Nan
作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center
摘要：Poly-ADP-ribose polymerase (PARP) family proteins are involved in a wide range of cellular processes. Several PARPs are targeted by inhibitors as treatments for cancer based on their biochemical functions; however, the physiological functions of most PARPs and the potential adverse effects of PARP inhibition are unknown. Here, we show that PARP7 is important for lung physiology. Loss of PARP7 in mice increases susceptibility to chemically induced diffuse alveolar hemorrhaging (DAH) and pristan...
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作者：Linnell, John D. C.
作者单位：Norwegian Institute Nature Research
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作者：Van De Velde, Sam; Yu, Jungting; Evensen, K. Garrett; Pakhlevanyan, Edmund; Williams, April E.; Shaw, Reuben J.; Montminy, Marc
作者单位：Salk Institute; Salk Institute; Salk Institute
摘要：Binding of GLP-1 to its receptor in pancreatic beta cells triggers activation of the cAMP pathway and phosphorylation of CREB, leading to induction of cellular target genes containing CREB binding sites. By contrast with their acute effects on beta cell gene expression, chronic exposure of beta cells to stable GLP-1 analogs like Exendin-4 stimulates sustained expression of beta cell-specific genes, leading to increases in beta cell viability and insulin secretion. In a proteomic screen for tra...
