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作者：Wu, Luyao; Wang, Jing; Xiao, Jun; Meng, Xinyao; Mao, Handan; You, Jingyi; Li, Zejian; Chen, Ke; Wang, Qiong; Zhou, Bingyan; Chen, Yingjian; Xiang, Lei; Yu, Xiaosi; Yang, Shimin; Zhang, Jiaxin; Zhuansun, Didi; Jiao, Chunlei; Wang, Di; Yang, Jixin; Chen, Xuyong; Feng, Jiexiong
作者单位：Huazhong University of Science & Technology
摘要：The formation of the enteric nervous system (ENS) primarily involves the migration of enteric neural crest-derived cells (ENCCs) and the subsequent maturation of enteric neurons. The developmental dysfunction of ENCCs and enteric neurons can result in ENS disorders, such as hypoganglionosis (HG). Although neurite outgrowth is fundamental to neuronal maturation, the mechanisms by which neurite outgrowth influences neuronal maturation remain poorly defined. Here, we identified EMB as a critical ...
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作者：Carlisle, Jolie A.; McCormick, Benjamin K.; Verbakel, Lina; von Philipsborn, Anne C.; Wolfner, Mariana F.; Clark, Andrew G.
作者单位：Cornell University; University of Fribourg
摘要：In Drosophila melanogaster, the male seminal fluid protein Sex Peptide regulates persistent postmating changes in female physiology and behavior. The persistence of long-term postmating responses in females requires Sex Peptide binding to sperm, which is mediated by a network of other seminal fluid proteins. A recent study documented substantial copy number variation of Sex Peptide genes in Drosophila species. We hypothesized that, due to shared selective pressures and compensatory changes to ...
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作者：Casper, W. Riley
作者单位：California State University System; California State University Fullerton
摘要：The (N+1)& times;(N+1) symmetric Pascal matrix TN is a generalized discrete time and band-limiting operator for the binomial transform and its eigenvectors are generalized discrete prolate spheroidal wave functions which we call binomial prolates. Their generating functions are also generalized prolate spheroidal functions in the sense that they are simultaneously eigenfunctions of a third-order differential operator and an integral operator over the line {z is an element of C: Re(z)=1/2}. For...
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作者：Ezomo, Ojeiru Felix; Suzuki, Rena; Narahashi, Maria; Matsuo, Satoshi; Inoue, Takeshi
作者单位：Tottori University
摘要：Behavioral decisions are elicited by environmental stimuli, but they are critically modulated by internal states. Persistent state-dependent biases are typically attributed to accumulated sensory history, high-intensity stimulation, or sustained environmental conditions. However, organisms also encounter isolated, innocuous perturbations whose long-term impacts remain poorly understood. Here, we show that a single brief mechanical cue induces changes in spontaneous locomotor activity in the pl...
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作者：Faulkner, Rebecca A.; Jo, Youngah; Garland-Brasher, Kristina; DeBose-Boyd, Russell A.
作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center
摘要：Two key enzymes, HMG CoA reductase (HMGCR) and squalene monooxygenase (SM), are subjected to distinct endoplasmic reticulum-associated degradation (ERAD) pathways to maintain cholesterol homeostasis. HMGCR catalyzes conversion of HMG CoA to mevalonate, the first rate-limiting step in cholesterol synthesis. Sterols accelerate ERAD of HMGCR by promoting its binding to Insig proteins, which recruit E3 ubiquitin ligases for ubiquitination and degradation. Downstream, SM catalyzes oxygenation of sq...
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作者：Parast, Saeid; Das, Madhurima; He, Yue; Wang, Simai; Gold, Sarah; Howard, Benjamin; Zeidner, Jacob; Philips, Cassandra; Ben-Sahra, Issam; Ramani, Vijay; Iwanaszko, Marta; Shilatifard, Ali
作者单位：Northwestern University; Feinberg School of Medicine; University of California System; University of California San Francisco; The J David Gladstone Institutes; University of California System; University of California San Francisco
摘要：Transcription by RNA polymerase II (RNAPII), which is essential for protein-coding gene expression and cellular function, is increasingly understood to become dysregulated with aging. Here, we use a multimodal approach to comprehensively characterize age-dependent changes in RNAPII-mediated transcription in both mouse and human tissues. Short-read total RNA sequencing (RNA-seq) to profile nascent transcription reveals a global reduction in overall transcriptional activity/frequency in aged tis...
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作者：Virgen, Gerardo A.; Skoko, Sulejman; Stingel, Ashley M.; Goswami, Tanmay; Cole, Richard L.; Crans, Debbie C.; Petersen, Poul B.; Verde, Ana Vila; Levinger, Nancy E.
作者单位：Colorado State University System; Colorado State University Fort Collins; University of Duisburg Essen; Ruhr University Bochum; Colorado State University System; Colorado State University Fort Collins; Colorado State University System; Colorado State University Fort Collins
摘要：The photoacid 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) is one of the most widely used fluorescent probes for studying proton transfer and local pH in systems from advanced materials to plants, environmental sensors to medicine. HPTS exists as two different species: the acid and its conjugate base, which lead to unique protonation-state-dependent translocation of the molecule when it is nanoconfined within anionic AOT reverse micelles. Using steady-state and time-resolved optical spectroscopy,...
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作者：Zhai, Guijin; Zhao, Fei; Wang, Yuhan; Jiang, Zixin; Li, Yanan; Fan, Zilong; Zheng, Wei; Han, Yanpu; Zhang, Jianji; Zang, Yong; Zhang, Kai
作者单位：Tianjin Medical University; Shandong Laboratory of Yantai Drug Discovery; Tianjin Medical University; Tianjin Medical University
摘要：Histone deacetylases (HDACs) regulate transcription and catalyze deacetylation predominantly within canonical transcriptional complexes. Nevertheless, the mechanistic role of other HDAC-associated proteins in orchestrating this process remains incompletely understood. To systematically decode endogenous HDAC interactomes in living cells, we developed BimPL, a heterobifunctional molecule-enabled proximity labeling strategy. Leveraging BimPL and quantitative proteomics, we robustly captured esta...
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作者：Albright, Ashley R.; Yan, Connie; Angeles-Albores, David; Hudnall, Yina; Makushok, Tatyana; Allen-Henderson, Jamarc; Marshall, Wallace F.
作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco
摘要：The molecular mechanisms that produce cell structure are poorly understood. The giant ciliate Stentor coeruleus is a unicellular model organism whose large size, reproducible structure, and ability to heal wounds and regenerate allow the formation of structure in a single cell to be addressed using methods of experimental embryology. Such studies have shown that specific cellular structures always form in particular regions of the cell, which raises the question: what is the source of position...
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作者：Bieschke, Jan
作者单位：University of London; UK Research & Innovation (UKRI); University College London; Medical Research Council UK (MRC)
