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作者：Pant, Vinod; Moyer, Sydney M.; Mitheera, V; Wasylishen, Amanda R.; Mirani, Akshita; Fowlkes, Natalie; Lozano, Guillermina
作者单位：University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center
摘要：The TP53 tumor suppressor encodes a transcription factor that regulates the expression of hundreds of target genes. Previous mouse studies have identified a conserved p53-dependent transcriptional signature that includes Cdkn1A (p21), Gtse1 (G2 and S-phase expressed 1), and Eda2r. In this study, we investigated the physiological roles of these three genes, along with Bbc3 (Puma), a p53 target involved in apoptosis, as effectors of p53 activity. We generated alleles of Gtse1 and Eda2r. In contr...
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作者：Villarreal, Elizabeth Sue; Marinho, Ygor; Loya, Omar; Aboagye, Samuel Yaw; Costa, Gabriela; Oliveira, Fernanda; Gupta, Muskan; Oliveira, Katia Costa; Silva, Claudia Lucia Martins; Sun, Jun; Erzurum, Serpil Cem; Lutz, Sarah Elizabeth; Williams, David Lee; Oliveira, Rudolf Feitoza Krawczenko; de Jesus Perez, Vinicio; Oliveira, Suellen Darc
作者单位：University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; Rush University; Universidade Federal de Sao Paulo (UNIFESP); University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; Universidade Federal de Sao Paulo (UNIFESP); University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital; Cleveland Clinic Foundation; Stanford University; University of Illinois System; University of Illinois Chicago; University of Illinois Chicago Hospital
摘要：Schistosomiasis-associated pulmonary hypertension (Sch-PH) is the most common form of group I PH worldwide. Recently, data revealed that the preclinical Sch-PH animal model exhibited gut and lung microbiome dysbiosis, associated with significant lung endothelial cell (EC) dysfunction and microvascular apoptosis. However, the role of pro-/antiapoptosis sensors, such as the inhibitor of apoptosis protein 2 (c-IAP2) and the purinergic receptor P2X7 (P2X7R), remained unclear. Using Cdh5cre-ERT2;cI...
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作者：Xu, Nan
作者单位：Ministry of Natural Resources of the People's Republic of China; Shenzhen University; Shenzhen University; Shenzhen University; Shenzhen University
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作者：Zheng, Juanjuan; Yang, Hang; Strandberg, Adam; Arunachalam, Easun; Ireland, William; Needleman, Daniel J.; Vlassak, Joost J.
作者单位：Harvard University; Harvard University; Simons Foundation; Flatiron Institute
摘要：While methods exist to indirectly quantify the metabolism of biological systems, directly measuring metabolic rates in living samples remains challenging. Here, we describe a calorimetric sensor with a sensitivity of similar to 100 pW at 23.1 mHz, suitable for measurements on living organisms, surpassing previously reported sensitivities. The sensor measures minute temperature differences between a capillary that contains the sample and two reference capillaries, directly relating this tempera...
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作者：Dhar, Matasha; Vazquez-Rosa, Edwin; Chaubey, Kalyani; Miller, Emiko; Corella, Sofia G.; Chakraborty, Suwarna; Tripathi, Sunil Jamuna; Das, Tapatee; Liao, Xudong; Alosman, Mohamed Alkassem; Fang, Hua; Koh, Yeojung; Sridharan, Preethy S.; Franke, Kathryn; Cintron-Perez, Coral J.; Cintron-Perez, Adrian A.; Tomco, Taylor; Indrakumar, Vidya; Rubin, Phoebe J.; Pieper, Justin G.; Ashiku, Luke A.; Shin, Min-Kyoo; Tang, Xinmiao; Padmanabhan, Roshan; Haragopal, Hariprakash; Flanagan, Margaret E.; Jain, Rajan; Winters, Bradley D.; Wilson, Brigid M.; Paul, Bindu D.; Jain, Mukesh K.; Pieper, Andrew A.
作者单位：University System of Ohio; Case Western Reserve University; University Hospitals of Cleveland; University System of Ohio; US Department of Veterans Affairs; Case Western Reserve University; Veterans Health Administration (VHA); Louis Stokes Cleveland Veterans Affairs Medical Center; University System of Ohio; Case Western Reserve University; University System of Ohio; Case Western Reserve University; Johns Hopkins University; Brown University; Massachusetts Institute of Technology (MIT); University System of Ohio; Case Western Reserve University; University of California System; University of California Santa Barbara; Northeastern University; Seoul National University (SNU); Seoul National University (SNU); Shanghai University of Traditional Chinese Medicine; University System of Ohio; Northeast Ohio Medical University (NEOMED); University of Texas System; University of Texas at San Antonio; University of Texas System; University of Texas at San Antonio; Northwestern University; Feinberg School of Medicine; University of Pennsylvania; University System of Ohio; Case Western Reserve University; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Brown University
摘要：Deterioration of the blood-brain barrier (BBB), including impaired neurovascular uncoupling, contributes to cognitive decline in aging. The BBB is formed principally by brain microvascular endothelial cells (ECs), and ECs throughout the body are enriched for the transcription factor Kr & uuml;ppel-like factor 4 (KLF4). Because KLF4 levels in ECs decrease with age, we tested whether that decline contributes to aging-related BBB deterioration, neurovascular dysfunction, and cognitive impairment....
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作者：Zulkifli, Mohammad; Farid, Ifrah; Oldfather, Laura E.; Shanbhag, Vinit C.; Leary, Scot C.; Petris, Michael J.; Cobine, Paul A.; Gohil, Vishal M.
作者单位：Texas A&M University System; Texas A&M University College Station; Auburn University System; Auburn University; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Saskatchewan
摘要：Copper (Cu) is an essential cofactor for cytochrome c oxidase (CcO), a mitochondrial respiratory chain enzyme that is metalated in the intermembrane space (IMS) primarily using Cu derived from the mitochondrial matrix pool. While Cu import into the matrix depends on the inner membrane carrier SLC25A3, the route by which matrix Cu is exported to the IMS for insertion into CcO has remained a major, unresolved step in intramitochondrial Cu trafficking. Here, we leveraged our recent discovery that...
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作者：Carr, Samuel C.; Wu, Song; Gase, Klaus; Nakamura, Yoko; Kamileen, Mohamed O.; Kunert, Maritta; Heinicke, Sarah; Kang, Moonyoung; Grabe, Veit; Caputi, Lorenzo; O'Connor, Sarah E.
作者单位：Max Planck Society; Max Planck Society
摘要：Monoterpene indole alkaloids encompass a diverse class of plant natural products. Many of these valuable compounds, including the anticancer medicines vinblastine and vincristine (Catharanthus roseus) and the antiaddiction treatment ibogaine (Tabernanthe iboga) utilize a common biosynthetic intermediate, 19E-geissoschizine, which is produced by the medium-chain dehydrogenase/reductase geissoschizine synthase (GS). Herein, we report facilitator of geissoschizine synthase (FoGS), a protein that ...
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作者：Ewart, Kyle M.; Adams, Maxim W. D.; Zhang, Zhuzhi; Baker, Louise; Fujiwara, Kokuto; Hayashi, Yoshinobu; Featherstone, Leo A.; Lu, Oscar Lo; Helbling, Jil E. S.; Moral, Maya; Maekawa, Kiyoto; Rose, Harley; Jex, Aaron; Ho, Simon Y. W.; Lo, Nathan
作者单位：University of Sydney; University of Edinburgh; UK Research & Innovation (UKRI); University of Edinburgh; Biotechnology and Biological Sciences Research Council (BBSRC); Roslin Institute; Walter & Eliza Hall Institute; National Institute of Advanced Industrial Science & Technology (AIST); Keio University; University of New South Wales Sydney; Kirby Institute; University of New South Wales Sydney; University of Toyama; University of Melbourne
摘要：Horizontal gene transfer (HGT) between organisms can be a valuable source of genetic variation and innovation. Research on HGT in eukaryotes has hitherto focused on transfers of coding sequences; insertions of noncoding DNA remain poorly understood. Here, we investigated HGT in cockroaches, which have a long-standing evolutionary relationship with the transovarially transmitted endosymbiont Blattabacterium cuenoti, making them a valuable system for assessing the potential scale of HGT. We alig...
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作者：Liu, Nan; Cheng, Mingrui; Tao, Yuqi; Sun, Xingchen; Wu, Boyi; Ma, Wenjing; Zhou, Xujiao; Hong, Jiaxu; Hu, Jingjing; Cheng, Yiyun
作者单位：East China Normal University; Fudan University; Southern Medical University - China
摘要：Fungal infections pose a growing global health challenge, exacerbated by a scarcity of effective treatments and rising drug resistance. Although cationic polymers emerge as promising antifungal candidates owing to structural tunability, design flexibility, and resistance to proteolytic degradation, their clinical utility has been hampered by nonselective membrane-disruption mechanisms. Herein, we develop a class of polycatechols- termed fungal iron predators (FIPs), exhibit exceptional fungici...
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作者：Mattingly, Jacob M.; Liponska, Anna; Tanquary, Julia; Yap, Mee-Ngan F.; Dunham, Christine M.
作者单位：Emory University; Emory University; Northwestern University; Feinberg School of Medicine; Emory University
摘要：Bacterial ribonucleases regulate gene expression in response to environmental stress and host interactions. In Staphylococcus aureus, the hibernation-promoting factor (Hpf) induces the formation of RNase R-resistant 100S ribosomes. We previously showed that the 3'-5' exoribonuclease YhaM cleaves the hpf transcript, reducing Hpf synthesis and leading to ribosome degradation. No structure of any YhaM homolog bound to RNA is available, and biological investigations of YhaM remain limited. Here, w...
