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作者：MacKinnon, Roderick; Haselwandter, Christoph A.
作者单位：Rockefeller University; University of Southern California; University of Southern California
摘要：Recent data suggest that many membrane proteins spontaneously organize into spatial patterns through weak noncovalent interactions. These weak interactions are protein type-specific and underlie the formation of higher-order transient structures (HOTS), which can function as 10 to 100 nanometer-sized, transient hubs of membrane signaling. We describe the necessary conditions for HOTS assembly to occur, its thermodynamic relationship to biomolecular condensate formation, and potential roles of ...
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作者：Wang, Yibo
作者单位：Beihang University; Hong Kong University of Science & Technology; Hong Kong University of Science & Technology
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作者：Wu, Man; Wu, Hao
作者单位：Harvard University; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Boston Children's Hospital; Program in Cellular & Molecular Medicine (PCMM)
摘要：The NLRP3 inflammasome is central to host defense and sterile inflammation and forms condensates at the microtubule-organizing center (also known as the centrosome), although the mechanisms regulating this process remain unclear. Here we define a functional relationship among microtubule transport, the centrosomal kinase NEK7, priming, and NLRP3 abundance. We show that microtubule-dependent transport is required for NEK7-dependent NLRP3 activation and promotes NEK7 to the pericentriolar materi...
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作者：Zentner, Aidan; Halingstad, Ethan V.; Chalk, Cameron; Brenner, Michael P.; Murugan, Arvind; Winfree, Erik; Shrinivas, Krishna
作者单位：Harvard University; Northwestern University; Northwestern University; California Institute of Technology; Harvard University; University of Chicago
摘要：Living organisms rely on molecular networks, such as gene circuits and signaling pathways, for information processing and robust decision-making in crowded, noisy environments. Recent advances show that interacting biomolecules self-organize by phase transitions into coexisting spatial compartments called condensates, often on cellular surfaces such as chromatin and membranes. In this paper, we demonstrate that multicomponent fluids can be designed to recruit distinct condensates to surfaces w...
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作者：Nihat, Akin; Arora, Parineeta; Schmidt, Christian; Rayner, Melissa L. D.; Linehan, Jacqueline; Brandner, Sebastian; Mead, Simon; Collinge, John; Jat, Parmjit S.
作者单位：University of London; UK Research & Innovation (UKRI); University College London; Medical Research Council UK (MRC); University College London Hospitals NHS Foundation Trust; University of London; National Hospital for Neurology & Neurosurgery; University College London; UCL Medical School; University of London; University College London; University of London School of Pharmacy; University College London Hospitals NHS Foundation Trust; University of London; National Hospital for Neurology & Neurosurgery; University College London; UCL Medical School; University of London; University College London
摘要：Prion diseases represent a unique biological paradigm with mechanistic parallels to other neurodegenerative conditions like Alzheimer's and Parkinson's diseases. However, the study of human prion pathobiology and the development of effective therapeutics has been severely constrained by the inability to propagate human prions in dividing cells-forcing reliance on costly and slow animal bioassays. Here, we report the generation of EKV cells-a humanized cell model which supports the robust, inde...
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作者：Sawers, R. Gary
作者单位：Martin Luther University Halle Wittenberg
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作者：Wang, Xuechun; Qin, Yujing; Duong, Loan D.; Liang, Minzhi; Chao, Adrian; Parrotta, Rossella; Li, Yaoyin; Purohit, Paresh Kumar; Fang, Yihao; Liu, Guoqiang; He, Jianping; Wen, Jiling; Liu, Yan; Zhang, Yuting; Zhao, Junling; Barupala, Nipuni; Schafer, Zachary T.; Lu, Xuemin; Szegezdi, Eva; Lu, Xin
作者单位：University of Notre Dame; University of Notre Dame; Ollscoil na Gaillimhe-University of Galway; University of Notre Dame; Indiana University System; Indiana University Indianapolis
摘要：Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive subtype of kidney cancer. Loss of von Hippel-Lindau (VHL) and the consequent activation of hypoxia-inducible factor-alpha (HIF alpha, especially HIF2 alpha) plays an essential role in ccRCC initiation and progression. The VHL-HIF2 alpha axis as the main driver for ccRCC may present specific opportunities to control the disease by cotargeting HIF2 alpha with belzutifan and another vulnerability. This study elucidates the ...
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作者：Cheng, Sizhe; Das, Devadyouti; Barchuk, Mykhaylo; Armstrong, Raveen; Klingbeil, Michele M.; Thomases, Becca; Zhou, Shuang
作者单位：University of Massachusetts System; University of Massachusetts Amherst; University of Massachusetts System; University of Massachusetts Amherst; Smith College
摘要：In the pathogenic parasite Trypanosoma brucei, a laterally attached flagellum drives rapid deformation of the complex cell body, producing puzzling dynamics. High-speed defocusing imaging reveals that surface points trace flower-like patterns in transverse planes. The petals arise from clockwise flagellar beating, which generates a right-handed helical wave propagating from the anterior tip along the body, advancing the cell like a twisted corkscrew. The central lobes result from slower counte...
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作者：Hartman, Kensington S.; Lopez, Bianca Y.; Gonzalez, Juan H.; Goetz, Madison E.; Cleveland, Aviel; Muroyama, Andrew
作者单位：University of California System; University of California San Diego
摘要：Stomata, microscopic pores that regulate gas exchange in plants, are patterned according to conserved pathways that regulate their physiology. Here, we identify a mode of stomatal patterning that depends on previously unrecognized regulation of the final symmetric cell division (SCD) that creates paired guard cells. SCDs are aligned by tensile stress at both the subcellular and supracellular scales, creating a globally polarized stomatal field that tracks the major axes of tissue growth. By id...
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作者：Hu, Xi; Liu, Xujiao; Wang, Yali; Liu, Ke; Gao, Hong; Zhu, Geqian; Wang, Shuai; Fang, Xiantao; Sun, Hong; Zhou, Fang; Ji, Shuiling; Zhou, Congzhao; Zhang, Yu; Xu, Xudong
作者单位：Central China Normal University; Chinese Academy of Sciences; Institute of Hydrobiology, CAS; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS
摘要：Cell differentiation and Turing-like patterning are tightly associated in a group of filamentous cyanobacteria that differentiate specialized N2-fixing cells, called heterocysts. Based on systematic genetic analyses, in particular genome-wide identification of recognized promoters and assays with a reconstituted Anabaena transcription system in Escherichia coli, we established HetZ as the central activator of the gene regulatory network of heterocyst differentiation. Biochemical and cryo-EM an...
