31期

最新文章

• Depletion-induced interactions modulate nanoscale protein diffusion in polymeric crowder solutions AI翻译
  作者：Dargasz, Michelle; Das Anthuparambil, Nimmi; Retzbach, Sebastian; Girelli, Anita; Timmermann, Sonja; Moller, Johannes; Jo, Wonhyuk; Leonau, Aliaksandr; Raza Agha, Mohammad; Bin, Maddalena; Savelkouls, Jaqueline; Andronis, Iason; Unger, Frederik; Brausse, Felix; Hallmann, Jorg; Boesenberg, Ulrike; Pudell, Jan-Etienne; Rodriguez-Fernandez, Angel; Wrigley, James; Shayduk, Roman; Youssef, Mohamed; Zozulya, Alexey; Madsen, Anders; Lehmkuhler, Felix; Perakis, Fivos; Zhang, Fajun; Schreiber, Frank; Paulus, Michael; Gutt, Christian
  作者单位：Universitat Siegen; Helmholtz Association; Deutsches Elektronen-Synchrotron (DESY); Eberhard Karls University of Tubingen; Stockholm University; European XFEL; Dortmund University of Technology
  摘要：Macromolecular crowding plays a crucial role in modulating protein dynamics in cellular and in vitro environments. Polymeric crowders such as dextran and Ficoll are known to induce entropic forces, including depletion interactions, that promote structural organization, yet their nanoscale consequences for protein dynamics remain poorly understood. Here, we employ megahertz X-ray photon correlation spectroscopy (MHz-XPCS) at the European X-ray Free Electron Laser (XFEL) to probe the dynamics of...
• Spatiotemporal control of Atg2 association with the ER during autophagosome formation AI翻译
  作者：Kotani, Tetsuya; Tanabe, Haruki; Kitta, Shinri; Higashi, Momoko; Niwa, Tatsuya; Kaminishi, Tatsuya; Kakuta, Chika; Shimasaki, Junko; Kosako, Hidetaka; Yoshimori, Tamotsu; Kuma, Akiko; Nakatogawa, Hitoshi
  作者单位：University of Osaka; University of Osaka; Tokushima University
  摘要：Autophagy degrades various intracellular components by sequestering them within membrane vesicles called autophagosomes and delivering them to lysosomes or vacuoles. Previous studies have revealed that the conserved, bridge-like lipid transfer protein Atg2 tethers autophagosome precursors to the endoplasmic reticulum (ER) and mediates lipid supply from the ER to drive their expansion into autophagosomes. However, how Atg2 docks onto the ER has remained unclear. Here, we show in Saccharomyces c...
• Dual-inhibitory mechanism of the bacterial retron Ec78 antiphage defense system AI翻译
  作者：Lin, Zhiying; Guo, Minghui; Lu, Zebin; Zhu, Yuwei; Zhou, Fengxia; Zhang, Anqi; Guo, Changyou; Huang, Zhiwei
  作者单位：Harbin Institute of Technology; Westlake University; Westlake Laboratory; Harbin Institute of Technology
  摘要：Retrons are prokaryotic defense modules that protect bacteria from phage infection through abortive infection. The retron Ec78 system employs a two-component effector complex PtuAB to execute this defense. Despite recent advances in structural research, the molecular mechanism by which PtuAB effector is regulated remains unknown. Here, we reveal that PtuAB is subject to a dual-inhibitory mechanism mediated by ATP/ADP and the RT-msDNA antitoxin. ATP/ADP binds nucleotide-binding domain (NBD) of ...
• Methionine oxidation alters both helical assembly and disordered contacts in human TDP-43 C-terminal domain phase separation AI翻译
  作者：Ozguney, Busra; Puterbaugh, Ryan Z.; Viswanathan, Renjith; Shenoy, Jayakrishna; Mohanty, Priyesh; Mittal, Jeetain; Fawzi, Nicolas L.
  作者单位：Texas A&M University System; Texas A&M University College Station; Brown University; Brown University; Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station
  摘要：TAR DNA binding protein 43 (TDP-43), a key protein linked to ALS pathology, undergoes phase separation and forms functional assemblies via condensation within cells. The conserved region (CR) within its C-terminal domain (CTD) mediates self-assembly through helix-helix interactions, while the flanking intrinsically disordered regions (IDRs) contribute to phase separation through transient interactions involving aromatic and hydrophobic residues. The CTD contains ten methionine residues distrib...
• Ancestral gene content estimates under gain-loss-duplication depend on the chosen observation threshold AI翻译
  作者：Szollosi, Gergely J.; Williams, Tom A.
  作者单位：Okinawa Institute of Science & Technology Graduate University; HUN-REN; HUN-REN Centre for Ecological Research; University of Bath
• An expanded apolipoprotein D family provides spider mites with dual-layer protection against dietary oxidative stress AI翻译
  作者：Wei, Si-Yu; An, Xin; Sun, Qin-Zhe; Yang, Qun; Han, Xi; Shi, Yu-Fei; Wang, Jin-Jun; Niu, Jinzhi
  作者单位：Southwest University - China; Southwest University - China; Gansu Agricultural University; Swedish University of Agricultural Sciences; Swedish University of Agricultural Sciences
  摘要：Plant defense has driven the evolution of species-dependent adaptive strategies in many herbivores, but the molecular mechanisms of adaptation in most arthropods remain largely unknown. The two-spotted spider mite (Tetranychus urticae) is a generalist herbivore that feeds by sucking the contents of mesophyll cells, a feeding strategy distinct from that of phloem-feeding insects. Here, we show the unexpected differential expression of apolipoprotein D (ApoD) genes during feeding when mites are ...
• Adaptive molecular convergence is pervasive across deep time and largely decoupled from phenotypic convergence AI翻译
  作者：Berger, Cory A.; Stoilova, Marina I.; Varney, Rebecca M.; Abrams, Sam C.; Miglietta, Maria Pia; Cartwright, Paulyn; Oakley, Todd H.
  作者单位：University of California System; University of California Santa Barbara; University of Kansas; University of Nebraska System; University of Nebraska Lincoln; University of California System; University of California Santa Barbara; Texas A&M University System; Texas A&M University College Station
  摘要：Reuse of homologous genes during the evolution of similar traits or ecological transitions is often taken as evidence that evolution is repeatable at the molecular level. To study gene reuse, biologists frequently select specific convergent phenotypes and search for signatures of natural selection in genomes associated with those phenotypes. However, the causes and frequency of genome-scale molecular convergence remain unresolved, especially over deep timescales. We use phylotranscriptomics an...
• Dynamic principles of concentration buffering through liquid-liquid phase separation AI翻译
  作者：de Monchaux-Irons, Logan; Tang, T. -Y. Dora; Weber, Christoph A.; Michaels, Thomas C. T.
  作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich; Swiss Federal Institutes of Technology Domain; ETH Zurich; Saarland University; University of Augsburg
  摘要：Living systems must maintain robust biochemical function despite fluctuations that span a wide range of timescales. Biomolecular condensates formed by liquid-liquid phase separation (LLPS) have been shown to buffer concentration fluctuations, but the principles governing their dynamic regulation remain unclear. We address this by probing the response of LLPS to oscillatory perturbations that mimic fluctuations across different timescales, establishing the first systematic frequency-domain anal...
• ICE arrests, 2015-2026: Variation in targeting, method, and geography AI翻译
  作者：East, Chloe N.; Patler, Caitlin; Cox, Elizabeth
  作者单位：University of Colorado System; University of Colorado Boulder; University of California System; University of California Berkeley
  摘要：We analyze and decompose administrative data on all 1.6 million US Immigration and Customs Enforcement (ICE) arrests from October 2015 to March 2026. Our results reveal that the reality of immigration enforcement diverges sharply from the public narrative that ICE arrests are necessary to protect public safety by removing people who commit crimes: Although arrests spiked at the outset of both Trump presidencies, the share of arrested individuals with criminal convictions fell significantly, wi...
• Parenthood decisions in uncertain times: Experimental evidence from four countries AI翻译
  作者：Aassve, Arnstein; Mencarini, Letizia; Peng, Chen; Plach, Samuel; Amarales, Alonso Roman; Tanturri, Maria Letizia
  作者单位：Bocconi University; Bocconi University; University of Padua
  摘要：Fertility rates are declining across much of the globe, yet the forces driving this decline have become increasingly complex, and classic theories have become less adequate to explain recent trends. While economic and gender-related explanations have long dominated fertility research, emerging global uncertainties, such as climate change, democratic backsliding, economic crises, and regional conflicts, may also shape how individuals evaluate childbearing. In addition to the more classical fact...