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作者：Ye, Shiwei; Gao, Yufeng; Deng, Mengying; Sheng, Yaozhang; Xu, Xingyun; Cao, Xueming; Liu, Lianjian; Xiao, Na; Zou, Junjie; Xie, Minghan; Zeng, Long; Xiang, Huachuang; Yu, Jia; Wu, Ting; He, Yuezhi; Yao, Jing; Li, Hui; Guo, Yanwu; Chu, Jun; Zheng, Hairong; Liu, Chengbo; Zheng, Wei
作者单位：Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS; Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS; Southern Medical University - China; Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS
摘要：Large-scale imaging of multiple dynamic behaviors and quantitative neurochemical concentrations with high spatiotemporal resolution is essential for understanding complex brain functions. Two-photon microscopy (TPM) is ideally suited for in vivo brain function imaging because of its high resolution and deep tissue penetration. However, conventional TPM is limited by a restricted field-of-view (FOV), an inherent trade-off between the imaging area and temporal resolution, and an insufficient amo...
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作者：He, Liqun; Johansson, Henrik J.; Vazquez-Liebanas, Elisa; Liu, Jianping; Muhl, Lars; Nahar, Khayrun; Vanlandewijck, Michael; Lehtio, Janne; Betsholtz, Christer; Andaloussi Mae, Maarja
作者单位：Uppsala University; Karolinska Institutet; Karolinska Institutet; University of Bergen
摘要：Current methods for assessing low-abundance proteins in individual cells are limited. As a result, cell functions are often inferred from single-cell RNA sequencing (scRNA-seq) data, which can be misleading due to the poor cross-gene correlation (different genes in the same cells) between messenger RNA (mRNA) and protein levels. To address this issue, we used isolated brain vascular fragments to study the blood-brain barrier. We applied a combination of deep bulk proteomic analysis, a proteomi...
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作者：Brooks, Jeremy P.; Jones, Andrew G.; Marcott, Shaun A.; Zoet, Lucas K.; Lifton, Nathaniel A.; Helanow, Christian; Caffee, Marc W.
作者单位：University of Wisconsin System; University of Wisconsin Madison; Purdue University System; Purdue University; Stockholm University; Purdue University System; Purdue University
摘要：Glaciers play an important role in shaping landscapes because of efficient bedrock erosion at their base. Understanding the contribution of glacial erosion to landscape evolution and the associated feedbacks between climate, erosion, and tectonics requires identifying the factors controlling glacial erosion. However, measuring glacial erosion rates is difficult due to the multifaceted combination of erosional processes occurring in glacial environments. Empirical validation of proposed control...
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作者：Yanai, Guy; Axel, Gabriel; Longo, Liam M.; Ben-Tal, Nir; Kolodny, Rachel
作者单位：University of Haifa; Tel Aviv University; Institute of Science Tokyo
摘要：Establishing a coherent mapping of the relationships among all known proteins is crucial for elucidating processes of protein emergence and evolution. Yet the capacity to fully capture relationships of protein similarity is complicated by the nonstraightforward interplay between sequence and structure; indeed, proteins with unrelated sequences can adopt similar structures, and, conversely, proteins with similar or identical sequences can manifest radically different structures. Here, we introd...
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作者：Zhu, Chen; Zhang, Weilong
作者单位：China Agricultural University; China Agricultural University; China Agricultural University; University of Cambridge; University of Cambridge
摘要：Excess sugar consumption is implicated in multiple cancers through insulin resistance, chronic inflammation, and metabolic reprogramming, yet whether early-life sugar exposure causally shapes long-term cancer risk remains an open question. We exploit the abrupt end of United Kingdom sugar rationing in September 1953 as a natural experiment, analyzing 64,761 UK Biobank participants born 1951-1956. Cohorts whose first 1,000 d of life fell under rationing exhibit dose-dependent reductions in adul...
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作者：Liu, Xiyuan; Tang, Dongmei; Zhang, Jing; Li, Tong; Huang, Jiaxiang; Qu, Jia; Ruan, Yijun; Chai, Haoxi; Chi, Zai-Long
作者单位：Wenzhou Medical University; Zhejiang University; Zhejiang University
摘要：The retinal pigment epithelium (RPE) is crucial for visual function, and its dysfunction contributes to retinal diseases such as age-related macular degeneration. Despite the translational potential of iPSC-derived RPE (iPSC-RPE) in cell replacement therapy, the functional visual gains achieved to date are modest. A key challenge is that the molecular and epigenomic signatures underlying functional RPE are yet to be fully elucidated. By integrating multiomics data, we systematically benchmarke...
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作者：Lohmar, Stephan; Rombach, Julius; Okwaro, Louis Allan; Zhou, Wei; Nehring, Volker; Korb, Judith
作者单位：University of Freiburg; University of Zurich
摘要：The evolution of life is characterized by major transitions, i.e., the evolution of new targets of selection (e.g., prokaryotes, eukaryotes, multicellular organisms, social insect colonies). They arose when preexisting lower-level units cooperated and gave up the capability to reproduce so that reproductive success is now achieved at the new, joint, higher unit. Currently, increasing confusion exists about major transition in evolution (MTE) that hampers progress in this fundamental research f...
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作者：Thangavel, Mohankumar; Masurkar, Arjun V.
作者单位：New York University; New York University
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作者：AbuSalim, Jenna E.; MacArthur, Michael M.; Gupta, Meera; Roichman, Asael; Hunter, Craig J.; Keber, Felix C.; Chatterjee, Seema; Moussavi, Mahta; Barouei, Javad; Wuhr, Martin; Sulakhe, Dinanath; Lehmann, Christopher J.; Donia, Mohamed S.; Rabinowitz, Joshua D.
作者单位：Princeton University; Princeton University; Princeton University; Ludwig Institute for Cancer Research; Princeton University; Bar Ilan University; University of Chicago; University of Chicago; University of Chicago Medical Center
摘要：Plant-based diets are associated with both positive health outcomes and a diverse gut microbiome. Such diets alter the microbiome's metabolic outputs, including increasing phenylalanine-derived phenols associated with beneficial health outcomes (hippuric acid and 3-phenylpropionate), while decreasing tyrosine-derived phenols considered uremic toxins (phenol sulfate and p-cresol sulfate). The mechanisms linking plant eating to these phenol metabolites are not known. Plant-based foods are fiber ...
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作者：Rosa-Oliveira, Lais; Ferreira Pires, Gabrielle; Gomes Tomaz, Ricardo; Pontes-Lopes, Aline; Maria Brumatti, Livia; Heil Costa, Marcos; Fonseca, Humberto Paiva; de Aragao, Luiz Eduardo Oliveira e Cruz
作者单位：Universidade Federal de Vicosa; Universidade Federal de Vicosa
摘要：Primary (PF) and secondary forests (SF) differ in structure, composition, and biophysical functioning. However, how these differences influence climate regulation across Amazonian landscapes remains unclear. Using annual climate and land cover data and a spatial mixed-effects framework, we quantified PF-SF contrasts in precipitation, evapotranspiration (ET), and land surface temperature (LST) across fractional forest cover gradients and SF successional stages in three Amazonian regions: centra...
