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作者：Listgarten, Jennifer; Jiang, Hanlun
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley
摘要：Over the past decades, protein engineering has matured into a field of its own, driven by computational modeling and high-throughput wet lab experiments, with broad application in therapeutics, diagnostics, agriculture, and manufacturing. In recent years, artificial intelligence (AI) has further propelled protein engineering by enabling more efficient search through high-dimensional sequence space for proteins with desired properties. Notable AI-based advances encompass generative modeling of ...
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作者：Widmann, Simon; Dam, Siddhartha; Dureth, Johannes; Mayer, Christian G.; Daviet, Romain; Zelle, Carl Philipp; Laibacher, David; Emmerling, Monika; Kamp, Martin; Diehl, Sebastian; Betzold, Simon; Klembt, Sebastian; Hofling, Sven
作者单位：University of Wurzburg; University of Cologne; Harvard University; University of Wurzburg
摘要：Equilibrium and nonequilibrium states of matter can exhibit fundamentally different behavior. A key example is the Kardar-Parisi-Zhang universality class in two spatial dimensions (2D KPZ), where microscopic deviations from equilibrium give rise to macroscopic scaling laws without equilibrium counterparts. Although extensively studied theoretically, experimental evidence of 2D KPZ scaling has remained limited to interface growth. Here, we report the observation of KPZ universal scaling in 2D e...
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作者：Alba, David M.; Arias-Martorell, Julia
作者单位：University of Barcelona; Institut Catala de Paleontologia Miquel Crusafont (ICP)
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作者：Blot, Guillaume; Sapieha, Przemyslaw
作者单位：Universite de Montreal; Universite de Montreal
摘要：Inflammation is transient, but its consequences can be lifelong. Even brief episodes of inflammation can lead to long-lasting changes in chromatin that control gene expression. These changes can poise cells to respond differently to subsequent inflammation episodes. For example, an initial encounter with environmental stressors, such as bacteria, viruses, or Western diets, durably tunes innate immune responses to later (related or unrelated) challenges (1, 2). These mechanisms also exist in no...
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作者：Voosen, Paul
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作者：Singla, Mamta; Wang, Yu Xin; Monti, Elena; Bedi, Yudhishtar; Agarwal, Pranay; Su, Shiqi; Ancel, Sara; Hermsmeier, Maiko; Devisetti, Nitya; Pandey, Akshay; Bakooshli, Mohsen Afshar; Palla, Adelaida R.; Goodman, Stuart; Blau, Helen M.; Bhutani, Nidhi
作者单位：Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; Sanford Burnham Prebys Medical Discovery Institute; Sanford Burnham Prebys Medical Discovery Institute
摘要：Aging or injury to the joints can lead to cartilage degeneration and osteoarthritis (OA), for which there are limited effective treatments. We found that expression of 15-hydroxy prostaglandin dehydrogenase (15-PGDH) is increased in the articular cartilage of aged or injured mice. Both systemic and local inhibition of 15-PGDH with a small-molecule inhibitor (PGDHi) led to regeneration of articular cartilage and reduction in OA-associated pain. Using single-cell RNA-sequencing and multiplexed i...
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作者：Barnett, Sam N.; Noseda, Michela
作者单位：Imperial College London; Imperial College London
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作者：Anthony, Colin J.; Lock, Colin; Johnson, Steven Manaoakamai; Maruyama, Shinichiro; Raymundo, Laurie J.
作者单位：University of Guam; University of Tokyo; University of Technology Sydney; Cornell University
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作者：Grimm, David
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作者：Schilling, Philipp E.; Steiner, Samuel; Bode, Jeffrey W.
作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich; Stanford University; Stanford Medicine
摘要：Chemical protein synthesis enables the construction of specific protein architectures but is limited to millimolar reaction concentrations, restricting access to poorly soluble proteins. Potassium acyltrifluoroboronates (KATs) offer a promising alternative through fast and chemoselective amide bond formation, but their application to protein synthesis has been precluded by the lack of a masking strategy. We report chiral, zwitterionic organoboron complexes that mask amino acid-derived KATs. Th...
