-
作者：Meng, Jun; Gilder, Stuart A.; Tan, Xiaodong; Li, Xin; Li, Yalin; Luo, Hui; Suzuki, Noritoshi; Wang, Zihao; Chi, Yuchen; Zhang, Chunyang; Wang, Chengshan
作者单位：China University of Geosciences; University of Munich; Chinese Academy of Sciences; South China Sea Institute of Oceanology, CAS; Chinese Academy of Sciences; Tohoku University
-
作者：Hernandez-Garcia, Jorge; Serrano-Mislata, Antonio; Lozano-Quiles, Maria; Urbez, Cristina; Nohales, Maria A.; Blanco-Tourinan, Noel; Peng, Huadong; Ledesma-Amaro, Rodrigo; Blazquez, Miguel A.
作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); Universitat Politecnica de Valencia; CSIC-UPV - Instituto de Biologia Molecular y Celular de Plantas (IBMCP); Wageningen University & Research; Imperial College London; Imperial College London
摘要：DELLA proteins are negative regulators of the gibberellin response pathway in angiosperms, acting as central hubs that interact with hundreds of transcription factors (TFs) and regulators to modulate their activities. While the mechanism of TF sequestration by DELLAs to prevent DNA binding to downstream targets has been extensively documented, the mechanism that allows them to act as coactivators remains to be understood. Here, we demonstrate that DELLAs directly recruit the Mediator complex t...
-
作者：Lavanchy, Eleonore; Weir, Bruce S.; Goudet, Jerome
作者单位：University of Lausanne; Swiss Institute of Bioinformatics; University of Lausanne; University of Washington; University of Washington Seattle
摘要：Measuring inbreeding and its consequences on fitness is central for many areas in biology including human genetics and the conservation of endangered species. However, there is no consensus on the best method, neither for quantification of inbreeding itself nor for the model to estimate its effect on specific traits. We simulated traits based on simulated genomes from a large pedigree and empirical whole-genome sequences of human data from populations with various sizes and structures (from th...
-
作者：Pollack, Dan; Nozoe, Takashi; Kussell, Edo
作者单位：New York University; University of Tokyo; University of Tokyo; University of Tokyo; New York University
摘要：Proteins that are kinetically stable are thought to be less prone to both aggregation and proteolysis. We demonstrate that the classical lac system of Escherichia coli can be leveraged as a model system to study this relation. beta- galactosidase (LacZ) plays a critical role in lactose metabolism and is an extremely stable protein that can persist in growing cells for multiple generations after expression has stopped. By attaching degradation tags to the LacZ protein, we find that LacZ can be ...
-
作者：Schnell, Eric; Muthukrishna, Michael
作者单位：University of London; London School Economics & Political Science
摘要：Previous models suggest that indirect reciprocity (reputation) can stabilize large - scale human cooperation [K. Panchanathan, R. Boyd, Nature 432 , 499-502 (2004)]. The logic behind these models and experiments [J. Gross et al. , Sci. Adv. 9, eadd8289 (2023) and O. P. Hauser, A. Hendriks, D. G. Rand, M. A. Nowak, Sci. Rep. 6, 36079 (2016)] is that a strategy in which individuals conditionally aid others based on their reputation for engaging in costly cooperative behavior serves as a punishme...
-
作者：Dong, Zaizai; Wang, Yusen; Xu, Gaolian; Liu, Bing; Wang, Yang; Reboud, Julien; Jajesniak, Pawel; Yan, Shi; Ma, Pingchuan; Liu, Feng; Zhou, Yuhao; Jin, Zhiyuan; Yang, Kuan; Huang, Zhaocun; Zhuo, Minglei; Jia, Bo; Fang, Jian; Zhang, Panpan; Wu, Nan; Yang, Mingzhu; Cooper, Jonathan M.; Chang, Lingqian
作者单位：Beihang University; Beihang University; University of Glasgow; Anhui Medical University
摘要：Accurate prediction of the efficacy of immunotherapy for cancer patients through the characterization of both genetic and phenotypic heterogeneity in individual patient cells holds great promise in informing targeted treatments, and ultimately in improving care pathways and clinical outcomes. Here, we describe the nanoplatform for interrogating living cell host - gene and (micro - )environment (NICHE) relationships, that integrates micro - and nanofluidics to enable highly efficient capture of...
-
作者：Sabet, Cameron John; Bajaj, Simar S.; Stanford, Fatima Cody
作者单位：Georgetown University; Harvard University; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University
-
作者：Zhou, Jie; Le, Chau Q.; Zhang, Yun; Wells, James A.
作者单位：University of Chicago; University of Chicago; University of California System; University of California San Francisco; Chan Zuckerberg Initiative (CZI); University of California System; University of California San Francisco
摘要：Directing antibodies to a particular epitope among many possible on a target protein is a significant challenge. Here, we present a simple and general method for epitope - directed selection (EDS) using a differential phage selection strategy. This involves engineering the protein of interest (POI) with the epitope of interest (EOI) mutated using a systematic bioinformatics algorithm to guide the local design of an EOI decoy variant. Using several alternating rounds of negative selection with ...
-
作者：Gilmore, Natalie; Tseng, Chieh- En J.; Maffei, Chiara; Tromly, Samantha L.; Deary, Katryna B.; Mckinney, Isabella R.; Kelemen, Jessica N.; Healy, Brian C.; Hu, Collin G.; Llorden, Gabriel Ramos -; Masood, Maryam; Cali, Ryan J.; Guo, Jennifer; Belanger, Heather G.; Yao, Eveline F.; Baxter, Timothy; Fischl, Bruce; Foulkes, Andrea S.; Polimeni, Jonathan R.; Rosen, Bruce R.; Perl, Daniel P.; Hooker, Jacob M.; Zuercher, Nicole R.; Huang, Susie Y.; Kimberly, W. Taylor; Greve, Douglas N.; Donald, Christine L. Mac; O'Connor, Kristen Dams -; Bodien, Yelena G.; Abc, Brian L. Edlow
作者单位：Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; State University System of Florida; University of South Florida; Harvard University; Harvard T.H. Chan School of Public Health; Uniformed Services University of the Health Sciences - USA; State University System of Florida; University of South Florida; Uniformed Services University of the Health Sciences - USA; University of Washington; University of Washington Seattle; Icahn School of Medicine at Mount Sinai; Icahn School of Medicine at Mount Sinai; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Spaulding Rehabilitation Hospital
摘要：United States (US) Special Operations Forces (SOF) are frequently exposed to explosive brain health are incompletely understood. Furthermore, there is no diagnostic test to detect brain injury from RBE. As a result, SOF personnel may experience cognitive, physical, and psychological symptoms for which the cause is never identified, and they may return to training or combat during a period of brain vulnerability. In 30 active- duty US SOF, we assessed the relationship between cumulative blast e...
-
作者：Lee, Tae Hoon; Elliott, Stephen
作者单位：Kyungpook National University (KNU); University of Oxford
