29期

最新文章

• Memory effects of prior subculture may impact the quality of multiomic perturbation profiles AI翻译
  作者：Bortel, Patricia; Hagn, Gerhard; Skos, Lukas; Bileck, Andrea; Paulitschke, Verena; Paulitschke, Philipp; Gleiter, Lion; Mohr, Thomas; Gerner, Christopher; Meier-Menches, Samuel M.
  作者单位：University of Vienna; University of Vienna; University of Vienna; Medical University of Vienna; Medical University of Vienna; University of Munich; Medical University of Vienna; University of Vienna
  摘要：Mass spectrometry- based omics technologies are increasingly used in perturbation studies to map drug effects to biological pathways by identifying significant molecular events. Significance is influenced by fold change and variation of each molecular parameter, but also by multiple testing corrections. While the fold change is largely determined by the biological system, the variation is determined by experimental workflows. Here, it is shown that memory effects of prior subculture can influe...
• Selective adsorption of high ionization potential value organic pollutants in wastewater AI翻译
  作者：Liang, Lihong; Cao, Jiazhen; Zhang, Yayun; Liu, Xinyue; Li, Jun; Yang, Bo; Lv, Weiyang; Yang, Qiang; Xing, Mingyang
  作者单位：East China University of Science & Technology; East China University of Science & Technology; East China University of Science & Technology; East China University of Science & Technology; Zhejiang Sci-Tech University
  摘要：It is imperative to devise effective removal strategies for high ionization potential (IP) organic pollutants in wastewater as their reduced electron-- donating capacity challenges the efficiency of advanced oxidation systems in degradation. Against this backdrop, leveraging the metal- based carbon material structure meticulously, we employed metal-- pyridine- N (M- N- C, M=Fe, Co, and Ni) as the electron transfer bridge. This distinctive design facilitated the ordered transfer of electrons fr...
• Point mutations at specific sites of the nsp12-nsp8 interface dramatically affect the RNA polymerization activity of SARS- CoV-2 AI翻译
  作者：Ferrer-Orta, Cristina; Vazquez-Monteagudo, Sergi; Ferrero, Diego S.; Martinez-Gonzalez, Brenda; Perales, Celia; Domingo, Esteban; Verdaguer, Nuria
  作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto de Biologia Molecular de Barcelona (IBMB); Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro Nacional de Biotecnologia (CNB); Autonomous University of Madrid; Autonomous University of Madrid; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro de Biologia Molecular Severo Ochoa (CBM)
  摘要：In a recent characterization of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-- CoV- 2)- 2) variability present in 30 diagnostic samples from patients of the first COVID- 19- 19 pandemic wave, 41 amino acid substitutions were documented in the RNA-- dependent RNA polymerase (RdRp) nsp12. Eight substitutions were selected in this work to determine whether they had an impact on the RdRp activity of the SARS- CoV- 2- CoV- 2 nsp12-nsp8-nsp7 replication complex. Three of these substitut...
• Palmitoylation at a conserved cysteine residue facilitates gasdermin D- mediated pyroptosis and cytokine release AI翻译
  作者：Liu, Zhonghua; Li, Sai; Wang, Chuanping; Vidmar, Kaylynn J.; Bracey, Syrena; Li, Ling; Willard, Belinda; Miyagi, Masaru; Lan, Tong; Dickinson, Bryan C.; Osme, Abdullah; Pizarro, Theresa T.; Xiao, Tsan Sam
  作者单位：University System of Ohio; Case Western Reserve University; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Cleveland Clinic Foundation; University System of Ohio; Case Western Reserve University; University of Chicago; University of Alabama System; University of Alabama Birmingham
  摘要：Gasdermin D (GSDMD)-- mediated pyroptotic cell death drives inflammatory cytokine release and downstream immune responses upon inflammasome activation, which play important roles in host defense and inflammatory disorders. Upon activation by pro- teases, the GSDMD N-- terminal domain (NTD) undergoes oligomerization and membrane translocation in the presence of lipids to assemble pores. Despite intensive studies, the molecular events underlying the transition of GSDMD from an autoinhibited solu...
• Control of G protein-coupled receptor function via membrane- interacting intrinsically disordered C- terminal domains AI翻译
  作者：Mancinelli, Chiara D.; Marx, Dagan C.; Gonzalez -Hernandez, Alberto J.; Huynh, Kevin; Mancinelli, Lucia; Arefin, Anisul; Khelashvilli, George; Levitz, Joshua; Eliezer, David
  作者单位：Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine
  摘要：G protein-coupled receptors (GPCRs) control intracellular signaling cascades via agonist-- dependent coupling to intracellular transducers including heterotrimeric G proteins, GPCR kinases (GRKs), and arrestins. In addition to their critical interactions with the transmembrane core of active GPCRs, all three classes of transducers have also been reported to interact with receptor C-- terminal domains (CTDs). An underexplored aspect of GPCR CTDs is their possible role as lipid sensors given the...
• The gender agency gap in fiction writing (1850 to 2010) AI翻译
  作者：Stuhler, Oscar
  作者单位：Northwestern University; Max Planck Society
  摘要：Works of fiction play a crucial role in the production of cultural stereotypes. Concerning gender, a widely held presumption is that many such works ascribe agency to men and passivity to women. However, large-- scale diachronic analyses of this notion have been lacking. This paper provides an assessment of agency attributions in 87,531 fiction works written between 1850 and 2010. It introduces a syntax- based approach for extracting networks of character interactions. Agency is then formalize...
• Electrifying HCOOH synthesis from CO2 building blocks over Cu-Bi nanorod arrays AI翻译
  作者：Zhang, Guiru; Tan, Bing; Mok, Dong Hyeon; Liu, Huiya; Ni, Baoxin; Zhao, Gui; Ye, Ke; Huo, Shengjuan; Miao, Xiaohe; Liang, Zheng; Liu, Xi; Chen, Liwei; Zhang, Zemin; Cai, Wen Bin; Back, Seoin; Jiang, Kun
  作者单位：Shanghai Jiao Tong University; Lanzhou University; Sogang University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai University; Westlake University; Shanghai Jiao Tong University; Ningxia University; Fudan University
  摘要：Precise electrochemical synthesis of commodity chemicals and fuels from CO2 2 building blocks provides a promising route to close the anthropogenic carbon cycle, in which renewable but intermittent electricity could be stored within the greenhouse gas molecules. Here, we report state- of- the- art CO2- 2- to- HCOOH- HCOOH valorization performance over a multiscale optimized Cu-Bi cathodic architecture, delivering a formate Faradaic efficiency exceeding 95% within an aqueous electrolyzer, a C- ...
• The integral role of de novo lipogenesis in the preparation for seasonal dormancy AI翻译
  作者：Cedden, Doga; Guney, Gozde; Toprak, Umut
  作者单位：Ankara University; University of Gottingen; University of Gottingen
  摘要：Animals can alter their body compositions in anticipation of dormancy to endure seasons with limited food availability. Accumulation of lipid reserves, mostly in the form of triglycerides (TAGs), is observed during the preparation for dormancy in diverse animals, including insects (diapause) and mammals (hibernation). However, the mechanisms involved in the regulation of lipid accumulation and the ecological consequences of failure to accumulate adequate lipid stores in preparation for animal ...
• A wound- induced differentiation trajectory for neurons AI翻译
  作者：Hulett, Ryan E.; Lopez, Carlos Rivera-; Gehrke, Andrew R.; Gompers, Annika; Srivastava, Mansi
  作者单位：Harvard University; Harvard University
  摘要：Animals capable of whole- body regeneration can replace any missing cell type and regenerate fully functional new organs, including new brains, de novo. The regeneration of a new brain requires the formation of diverse neural cell types and their assembly into an organized structure with correctly wired circuits. Recent work in various regenerative animals has revealed transcriptional programs required for the differentiation of distinct neural subpopulations, however, how these transcriptiona...
• Recombinant OC43 SARS-CoV-2 spike replacement virus: An improved BSL-2 proxy virus for SARS-CoV-2 neutralization assays AI翻译
  作者：Hu, Zhe; Lopez-Munoz, Alberto Domingo; Kosik, Ivan; Li, Tiansheng; Callahan, Victoria; Brooks, Kelsie; Yee, Debra S.; Holly, Jaroslav; Santos, Jefferson J. S.; Brant, Ayslan Castro; Johnson, Reed F.; Takeda, Kazuyo; Zheng, Zhi - Ming; Brenchley, Jason M.; Yewdell, Jonathan W.; Fox, Julie M.
  作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID); National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID); National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID); National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID); US Food & Drug Administration (FDA); Center for Biologics Evaluation & Research (CBER)
  摘要：We generated a replication-- competent OC43 human seasonal coronavirus (CoV) expressing the severe acute respiratory syndrome coronavirus 2 (SARS-- CoV-2)- 2) spike in place of the native spike (rOC43-CoV2- CoV2 S). This virus is highly attenuated relative to OC43 and SARS-CoV-2- CoV- 2 in cultured cells and animals and is classified as a biosafety level 2 (BSL-2)- 2) agent by the NIH biosafety committee. Neutralization of rOC43-CoV2- CoV2 S and SARS-CoV-2- CoV- 2 by S-- specific monoclonal an...