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作者：Johnston, Kadina E.; Almhjell, Patrick J.; Watkins-Dulaney, Ella J.; Liu, Grace; Porter, Nicholas J.; Yang, Jason; Arnold, Frances H.
作者单位：Merck & Company; Merck & Company USA; Stanford University; Harvard University; Harvard Medical School; California Institute of Technology; California Institute of Technology
摘要：Protein engineering often targets binding pockets or active sites which are enriched in epistasis-nonadditive interactions between amino acid substitutions-and where the combined effects of multiple single substitutions are difficult to predict. Few existing sequence- fitness datasets capture epistasis at large scale, especially for enzyme catalysis, limiting the development and assessment of model- guided enzyme engineering approaches. We present here a combinatorially complete, 160,000- vari...
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作者：Teng, Xiao-Yu; Hu, Ping; Zhang, Cai-Ming; Zhang, Qin-Xin; Yang, Guolin; Zang, Yan-Yu; Liu, Zhi-Xiong; Chen, Guiquan; Shi, Yun Stone
作者单位：Nanjing University; Nanjing Medical University; Southern Medical University - China
摘要：a critical role in myelination; dysfunction of this protein leads to hypomyelination and white matter abnormalities (WMAs). Here, we hypothesized that LGI1 may regulate myelination through binding to an unidentified receptor on the membrane of oligodendrocytes (OLs). To search for this hypothetic receptor, we analyzed LGI1 binding followed by mass spectrometry. An OL- specific membrane protein, the oligodendrocytic myelin paranodal and inner loop protein (OPALIN), was identified. Conditional k...
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作者：Toubdji, Sabrine; Thullier, Quentin; Kilz, Lea - Marie; Marchand, Virginie; Yuan, Yifeng; Sudol, Claudia; Goyenvalle, Catherine; Jean, Olivier Jean-; Rose, Simon; Douthwaite, Stephen; Hardy, Leo; Baharoglu, Zeynep; de Crecy-Lagard, Valerie; Helm, Mark; Motorin, Yuri; Hamdane, Djemel; Bregeon, Damien
作者单位：Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); Universite PSL; College de France; Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); Universite de Lorraine; Universite de Lorraine; Centre National de la Recherche Scientifique (CNRS); Johannes Gutenberg University of Mainz; State University System of Florida; University of Florida; University of Southern Denmark; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; State University System of Florida; University of Florida
摘要：Dihydrouridine (D), a prevalent and evolutionarily conserved base in the transcriptome, primarily resides in tRNAs and, to a lesser extent, in mRNAs. Notably, this modification is found at position 2449 in the Escherichia coli 23S rRNA, strategically positioned near the ribosome's peptidyl transferase site. Despite the prior identification, in E. coli genome, of three dihydrouridine synthases (DUS), a set of NADPH and FMN- dependent enzymes known for introducing D in tRNAs and mRNAs, character...
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作者：Zhang, Wei; Shi, Ke; Hsueh, Fu - Chun; Mendoza, Alise; Ye, Gang; Huang, Linfen; Perlman, Stanley; Aihara, Hideki; Li, Fang
作者单位：University of Minnesota System; University of Minnesota Twin Cities; University of Minnesota System; University of Minnesota Twin Cities; University of Minnesota System; University of Minnesota Twin Cities; University of Iowa
摘要：The animal origin of SARS-CoV-2 remains elusive, lacking a plausible evolutionary narrative that may account for its emergence. Its spike protein resembles certain segments of BANAL- 236 and RaTG13, two bat coronaviruses considered possible progenitors of SARS- CoV-2. Additionally, its spike contains a furin motif, a common feature of rodent coronaviruses. To explore the possible involvement of rodents in the emergence of SARS-CoV-2 spike, we examined the crystal structures of the spike recept...
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作者：Greenberg, Miriam; Angelo, Hillary; Losada, Elena; Wilmers, Christopher C.
作者单位：University of California System; University of California Santa Cruz; University of California System; University of California Santa Cruz
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作者：Kern, Vanessa R.; Carlson, Andreas
作者单位：University of Oslo
摘要：Drop-fiber interactions are fundamental to the operation of technologies such as atmospheric fog capture, oil filtration, refrigeration, and dehumidification. We demonstrate that by twisting together two fibers, a sliding drop's flow path can be controlled by tuning the ratio between its size and the twist wavelength. We find both experimentally and numerically that twisted fiber systems are able to asymmetrically stabilize drops, both enhancing drop transport speeds and creating a rich array ...
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作者：Kudruk, Sergej; Forsyth, Connor M.; Dion, Michelle Z.; Orbeck, Jenny K. Hedlund; Luo, Jingqin; Klein, Robyn S.; Kim, Albert H.; Heimberger, Amy B.; Mirkin, Chad A.; Stegh, Alexander H.; Artzi, Natalie
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作者：Lim, Justin J.; Vining, Kyle H.; Mooney, David J.; Blencowe, Benjamin J.
作者单位：University of Toronto; University of Toronto; University of Pennsylvania; University of Pennsylvania; Harvard University; Harvard University
摘要：Cell-matrix interactions in 3D environments significantly differ from those in 2D cultures. As such, mechanisms of mechanotransduction in 2D cultures are not necessarily applicable to cell- encapsulating hydrogels that resemble features of tissue architecture. Accordingly, the characterization of molecular pathways in 3D matrices is expected to uncover insights into how cells respond to their mechanical environment in physiological contexts, and potentially also inform hydrogel-based strategie...
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作者：Plascencia-Villa, German; Castellani, Rudolph J.; Perry, George
作者单位：University of Texas System; University of Texas at San Antonio; Northwestern University; Feinberg School of Medicine
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作者：Costa, Antonio C.; Ahamed, Tosif; Jordan, David; Stephens, Greg J.
作者单位：Vrije Universiteit Amsterdam; Howard Hughes Medical Institute; University of Cambridge; Okinawa Institute of Science & Technology Graduate University; Universite Paris Cite; Universite PSL; Ecole Normale Superieure (ENS); Centre National de la Recherche Scientifique (CNRS); Sorbonne Universite
摘要：How do we capture the breadth of behavior in animal movement, from rapid body twitches to aging? Using high-resolution videos of the nematode worm Caenorhabditis elegans, we show that a single dynamics connects posture-scale fluctuations with trajectory diffusion and longer-lived behavioral states. We take short posture sequences as an instantaneous behavioral measure, fixing the sequence length for maximal prediction. Within the space of posture sequences, we construct a fine-scale, maximum e...
