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作者：Morales, Maryann; Yang, Moon Young; Goddard, William A., III; Gray, Harry B.; Winkler, Jay R.
作者单位：California Institute of Technology
摘要：The intrinsically disordered C - terminal peptide region of severe acute respiratory syndrome coronavirus 2 nonstructural protein - 1 (Nsp1 - CT) inhibits host protein synthesis by blocking messenger RNA (mRNA) access to the 40S ribosome entrance tunnel. Aqueous copper(II) ions bind to the disordered peptide with micromolar affinity, creating a possible strategy to restore protein synthesis during host infection. Electron paramagnetic resonance (EPR) and tryptophan fluorescence measurements on...
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作者：Jhaveri, Adip; Loggia, Spencer; Qian, Yian; Johnson, Margaret E.
作者单位：Johns Hopkins University
摘要：Macromolecular complexes are often composed of diverse subunits. The self - assembly of these subunits is inherently nonequilibrium and must avoid kinetic traps to achieve high yield over feasible timescales. We show how the kinetics of self - assembly benefits from diversity in subunits because it generates an expansive parameter space that naturally improves the expressivity of self - assembly, much like a deeper neural network. By using automatic differentiation algorithms commonly used in ...
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作者：Kim, Dongho; Cianfrocco, Michael A.; Verhey, Kristen J.; Smith, Gregory A.; Shenk, Thomas
作者单位：Northwestern University; Feinberg School of Medicine; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
摘要：Neurotropic alphaherpesviruses, including herpes simplex virus type 1 (HSV - 1), recruit microtubule motor proteins to invade cells. The incoming viral particle traffics to nuclei in a two - step process. First, the particle uses the dynein-dynactin motor to sustain transport to the centrosome. In neurons, this step is responsible for long - distance retrograde axonal transport and is an important component of the neuroinvasive property shared by these viruses. Second, a kinesin - dependent me...
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作者：Nasello, Cara; Poppi, Lauren A.; Wu, Junbing; Kowalski, Tess F.; Thackray, Joshua K.; Wang, Riley; Persaud, Angelina; Mahboob, Mariam; Lin, Sherry; Spaseska, Rodna; Johnson, C. K.; Gordon, Derek; Tissir, Fadel; Heiman, Gary A.; Tischfield, Jay A.; Bocarsly, Miriam; Tischfield, Max A.
作者单位：Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; Harvard University; Harvard Medical School; Qatar Foundation (QF); Hamad Bin Khalifa University-Qatar; Universite Catholique Louvain
摘要：Tourette disorder (TD) is poorly understood, despite affecting 1/160 children. A lack of animal models possessing construct, face, and predictive validity hinders progress in the field. We used CRISPR/Cas9 genome editing to generate mice with mutations orthologous to human de novo variants in two high - confidence Tourette genes, CELSR3 and WWC1 . Mice with human mutations in Celsr3 and Wwc1 exhibit cognitive and/or sensorimotor behavioral phenotypes consistent with TD. Sensorimotor gating def...
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作者：Wu, Tianze; Ge, Jingjie; Wu, Qian; Ren, Xiao; Meng, Fanxu; Wang, Jiarui; Xi, Shibo; Wang, Xin; Elouarzaki, Kamal; Fisher, Adrian; Xu, Zhichuan J.
作者单位：Nanyang Technological University; Hong Kong Polytechnic University; Peking University; Agency for Science Technology & Research (A*STAR); City University of Hong Kong; Nanyang Technological University; University of Cambridge; Nanyang Technological University
摘要：Water oxidation on magnetic catalysts has generated significant interest due to the spin - polarization effect. Recent studies have revealed that the disappearance of magnetic domain wall upon magnetization is responsible for the observed oxygen evolution reaction (OER) enhancement. However, an atomic picture of the reaction pathway remains unclear, i.e., which reaction pathway benefits most from spin - polarization, the adsorbent evolution mechanism, the intermolecular mechanism (I2M), the la...
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作者：Xiao, Chufan; Liu, Xiufang; Pan, Yuyang; Li, Yanling; Qin, Ling; Yan, Zhibo; Feng, Yunzi; Zhao, Mouming; Huang, Mingtao
作者单位：South China University of Technology
摘要：Genetic elements are foundational in synthetic biology serving as vital building blocks. They enable programming host cells for efficient production of valuable chemicals and recombinant proteins. The unfolded protein response (UPR) is a stress pathway in which the transcription factor Hac1 interacts with the upstream unfolded protein response element (UPRE) of the promoter to restore endoplasmic reticulum (ER) homeostasis. Here, we created a UPRE2 mutant (UPRE2m) library. Several rounds of sc...
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作者：Bellani, Luna; Ceolotto, Stefano; Elsner, Benjamin; Pestel, Nico
作者单位：Ulm University; IZA Institute Labor Economics; Centro Euro-Mediterraneo sui Cambiamenti Climatici (CMCC); Universita Ca Foscari Venezia; University College Dublin; University of London; University College London; Maastricht University; Leibniz Association; Ifo Institut
摘要：This paper studies the effect of air pollution on voting outcomes. We use data from 60 federal and state elections in Germany from 2000 to 2018 and exploit plausibly exogenous fluctuations in ambient air pollution within counties across election dates. Higher air pollution on election day shifts votes away from incumbent parties and toward opposition parties. An increase in the concentration of particulate matter (PM10) by 10 mu g/m 3 -around two within -county SDs-reduces the vote share of in...
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作者：Diem, Stephanie J.; Bayon, Baindu L.; Mahmoud, Hussam; Garcia-Cazarin, Mary L.; Martin, Michael J.; Rittschof, Clare C.; Silveyra, Patricia; Boland -Reeves, Alison; Najib, Dalal; Wasson, Flannery
作者单位：National Academies of Sciences, Engineering & Medicine; University of Wisconsin System; University of Wisconsin Madison; Saint Mary's College of California; Colorado State University System; Colorado State University Fort Collins; University of Kentucky; Indiana University System; Indiana University Bloomington; National Academies of Sciences, Engineering & Medicine
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作者：He, Qing; Wang, Feng; O'Donnell, Michael E.; Li, Huilin
作者单位：Van Andel Institute; Rockefeller University; Howard Hughes Medical Institute; Rockefeller University
摘要：The DNA sliding clamp PCNA is a multipurpose platform for DNA polymerases and many other proteins involved in DNA metabolism. The topologically closed PCNA ring needs to be cracked open and loaded onto DNA by a clamp loader, e.g., the well - studied pentameric ATPase complex RFC (RFC1 - 5). The CTF18RFC complex is an alternative clamp loader found recently to bind the leading strand DNA polymerase epsilon and load PCNA onto leading strand DNA, but its structure and the loading mechanism have b...
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作者：Jasti, Naga Prathibha; Levine, Igal; Feldman, Vishay (Isai); Hodes, Gary; Aharon, Sigalit; Cahen, David
作者单位：Bar Ilan University; Bar Ilan University; Weizmann Institute of Science; Helmholtz Association; Helmholtz-Zentrum fuer Materialien und Energie GmbH (HZB); Weizmann Institute of Science; Hebrew University of Jerusalem
摘要：The term defect tolerance (DT) is used often to rationalize the exceptional optoelectronic properties of halide perovskites (HaPs) and their devices. Even though DT lacked direct experimental evidence, it became a fact in the field. DT in semiconductors implies that structural defects do not translate to electrical and optical effects (e.g., due to charge trapping), associated with such defects. We present pioneering direct experimental evidence for DT in Pb- HaPs by comparing the structural q...
