-
作者：Stevens, Danielle M.; Moreno-Perez, Alba; Weisberg, Alexandra J.; Ramsing, Charis; Fliegmann, Judith; Zhang, Ning; Madrigal, Melanie; Martin, Gregory; Steinbrenner, Adam; Felix, Georg; Coaker, Gitta
作者单位：University of California System; University of California Davis; University of California System; University of California Davis; Oregon State University; Eberhard Karls University of Tubingen; Cornell University; Boyce Thompson Institute for Plant Research; Cornell University; University of Washington; University of Washington Seattle
摘要：Plants and animals detect biomolecules termed microbe - associated molecular patterns (MAMPs) and induce immunity. Agricultural production is severely impacted by pathogens which can be controlled by transferring immune receptors. However, most studies use a single MAMP epitope and the impact of diverse multicopy MAMPs on immune induction is unknown. Here, we characterized the epitope landscape from five proteinaceous MAMPs across 4,228 plant - associated bacterial genomes. Despite the diversi...
-
作者：Castanha, Priscila M. S.; McEnaney, Patrick J.; Park, Yongseok; Bouwer, Anthea; Chaves, Elton J. F.; Lins, Roberto D.; Paciaroni, Nicholas G.; Dickson, Paige; Carlson, Graham; Cordeiro, Marli T.; Magalhaes, Tereza; Craigo, Jodi; Marques, Ernesto T. A.; Kodadek, Thomas; Burke, Donald S.
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Universidade Federal de Pernambuco; Fundacao Oswaldo Cruz; Texas A&M University System; Texas A&M University College Station; Universidade Federal da Bahia; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh
摘要：Antigenic similarities between Zika virus (ZIKV) and other flaviviruses pose challenges to the development of virus-specific diagnostic tools and effective vaccines. Starting with a DNA-encoded one-bead-one-compound combinatorial library of 508,032 synthetic, non-natural oligomers, we selected and characterized small molecules that mimic ZIKV epitopes. High-throughput fluorescence-activated cell sorter-based bead screening was used to select molecules that bound IgG from ZIKV-immune but not fr...
-
作者：Chai, Mengzhu; Li, Lei; Li, Yong; Yang, Yingshuai; Wang, Yuting; Jiang, Xue; Luan, Yameng; Li, Fangfang; Cui, Hongguang; Wang, Aiming; Xiang, Wensheng; Wu, Xiaoyun; Cheng, Xiaofei
作者单位：Northeast Agricultural University - China; Northeast Agricultural University - China; Chinese Academy of Agricultural Sciences; Institute of Plant Protection, CAAS; Hainan University; Agriculture & Agri Food Canada
摘要：Potyviridae, the largest family of plant RNA viruses, includes many important pathogens that significantly reduce the yields of many crops worldwide. In this study, we report that the 6-kilodalton peptide 1 (6K1), one of the least characterized potyviral proteins, is an endoplasmic reticulum-localized protein. AI-assisted structure modeling and biochemical assays suggest that 6K1 forms pentamers with a central hydrophobic tunnel, can increase the cell membrane permeability of Escherichia coli ...
-
作者：Geisberg, Joseph, V; Moqtaderi, Zarmik; Struhl, Kevin
作者单位：Harvard University; Harvard Medical School
摘要：The RNA polymerase II (Pol II) elongation rate influences poly(A) site selection, with slow and fast Pol II derivatives causing upstream and downstream shifts, respectively, in poly(A) site utilization. In yeast, depletion of either of the histone chaperones FACT or Spt6 causes an upstream shift of poly(A) site use that strongly resembles the poly(A) profiles of slow Pol II mutant strains. Like slow Pol II mutant strains, FACT - and Spt6 - depleted cells exhibit Pol II processivity defects, in...
-
作者：Jaafari, Hana; Bueno, Carlos; Schafer, Nicholas P.; Martin, Jonathan; Morcos, Faruck; Wolynes, Peter G.
作者单位：Rice University; Rice University; Rice University; University of Texas System; University of Texas Dallas; University of Texas System; University of Texas Dallas; University of Texas System; University of Texas Dallas; Rice University; Rice University
摘要：Protein evolution is guided by structural, functional, and dynamical constraints ensuring organismal viability. Pseudogenes are genomic sequences identified in many eukaryotes that lack translational activity due to sequence degradation and thus over time have undergone devolution. Previously pseudogenized genes sometimes regain their protein-coding function, suggesting they may still encode robust folding energy landscapes despite multiple mutations. We study both the physical folding landsca...
-
作者：Lafuente-Bartolome, Jon; Lian, Chao; Giustino, Feliciano
作者单位：University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin
摘要：Halide perovskites emerged as a revolutionary family of high -quality semiconductors for solar energy harvesting and energy -efficient lighting. There is mounting evidence that the exceptional optoelectronic properties of these materials could stem from unconventional electron-phonon couplings, and it has been suggested that the formation of polarons and self -trapped excitons could be key to understanding such properties. By performing first -principles simulations across the length scales, h...
-
作者：Sieh, Kerry; Herrin, Jason; Angel, Dayana Schonwalder
作者单位：Nanyang Technological University; Nanyang Technological University; National Taiwan University; Academia Sinica - Taiwan; Nanyang Technological University; Universidad Industrial de Santander
-
作者：Su, Qi; Xu, Bing; Chen, Xin; Rokita, Steven E.
作者单位：University of California System; University of California San Diego; Stanford University; Johns Hopkins University; Howard Hughes Medical Institute; Johns Hopkins University; Johns Hopkins University
摘要：Biological regulation often depends on reversible reactions such as phosphorylation, acylation, methylation, and glycosylation, but rarely halogenation. A notable exception is the iodination and deiodination of thyroid hormones. Here, we report detection of bromotyrosine and its subsequent debromination during Drosophila spermatogenesis. Bromotyrosine is not evident when Drosophila express a native flavin- dependent dehalogenase that is homologous to the enzyme responsible for iodide salvage f...
-
作者：Xiao, Yi; Guo, Zhichao; Cao, Jing; Song, Ping; Yang, Bo; Xu, Weilin
作者单位：Chinese Academy of Sciences; Changchun Institute of Applied Chemistry, CAS; Chinese Academy of Sciences; Changchun Institute of Applied Chemistry, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; ShanghaiTech University
摘要：Understanding the operando defect-tuning performance of catalysts is critical to establish an accurate structure-activity relationship of a catalyst. Here, with the tool of single - molecule super - resolution fluorescence microscopy, by imaging intermediate CO formation/oxidation during the methanol oxidation reaction process on individual defective Pt nanotubes, we reveal that the fresh Pt ends with more defects are more active and anti - CO poisoning than fresh center areas with less defect...
-
作者：Han, Yue; Li, Min; Zhao, Bingqing; Wang, Huichao; Liu, Yan; Liu, Zhijun; Xu, Jiaxi; Yang, Rui
作者单位：Xi'an Jiaotong University; Xi'an Jiaotong University; Xi'an Jiaotong University; Oregon Health & Science University
摘要：Neurons regulate the microtubule - based transport of certain vesicles selectively into axons or dendrites to ensure proper polarization of function. The mechanism of this polarized vesicle transport is still not fully elucidated, though it is known to involve kinesins, which drive anterograde transport on microtubules. Here, we explore how the kinesin - 3 family member KIF13A is regulated such that vesicles containing transferrin receptor (TfR) travel only to dendrites. In experiments involvi...
