MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize vesicular transport of transferrin receptor within dendrites

• 成果类型: Article
• 署名作者: Han, Yue; Li, Min; Zhao, Bingqing; Wang, Huichao; Liu, Yan; Liu, Zhijun; Xu, Jiaxi; Yang, Rui
• 署名单位: Xi'an Jiaotong University; Xi'an Jiaotong University; Xi'an Jiaotong University; Oregon Health & Science University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12365
• DOI: 10.1073/pnas.2316266121
• 发表日期: 2024-05-14
• 关键词: axon initial segment; cargo transport; proteins; motor; dynein; mechanisms; kinesins; reveals

摘要

Neurons regulate the microtubule - based transport of certain vesicles selectively into axons or dendrites to ensure proper polarization of function. The mechanism of this polarized vesicle transport is still not fully elucidated, though it is known to involve kinesins, which drive anterograde transport on microtubules. Here, we explore how the kinesin - 3 family member KIF13A is regulated such that vesicles containing transferrin receptor (TfR) travel only to dendrites. In experiments involving live - cell imaging, knockout of KIF13A, BioID assay, we found that the kinase MARK2 phosphorylates KIF13A at a 14 - 3 - 3 binding motif, strengthening interaction of KIF13A with 14 - 3 - 3 such that it dissociates from TfR - containing vesicles, which therefore cannot enter axons. Overexpression of KIF13A or knockout of MARK2 leads to axonal transport of TfR - containing vesicles. These results suggest a unique kinesin - based mechanism for polarized transport of vesicles to dendrites.