2025

最新文章

• Indigenous fire stewardship shaped North American Great Lakes forests AI翻译
  作者：Larson, Evan R.; Montano, Nisogaabokwe Melonee; Lockling, Emily; Ojibway, Ashla; Reynolds, Mocha B.; Zhaawendaagozikwe, Valerie Ross; Johnson, Lane B.; Kipfmueller, Kurt F.; Dockry, Michael J.; Northrup, Vern; Savage, Jeff; Kimmerer, Robin Wall
  作者单位：University of Minnesota System; University of Minnesota Twin Cities; University of Minnesota System; University of Minnesota System; University of Minnesota Twin Cities; State University of New York (SUNY) System; State University of New York (SUNY) College of Environmental Science & Forestry
  摘要：Interest in bringing Traditional Ecological Knowledge (TEK) and Western Science together to enhance climate and landscape resilience is growing rapidly, particularly for engagement with pyrogenic communities around the world. For these systems, Indigenous Knowledge offers unique insights that reflect millennia of intimate engagement with local landscapes, while Western Science can offer additional understanding of the emergence of climates and ecological conditions that are novel at evolutiona...
• Structural basis and affinity improvement for an ATP-binding AI翻译
  作者：Jiang, Yan; Zhang, Yuchao; Wan, Liqi; Cui, Cheng; Guo, Pei; Han, Da; Tan, Weihong; Patel, Dinshaw
  作者单位：Hunan University; Chinese Academy of Sciences; Hangzhou Institute of Medicine, CAS; Shanghai Jiao Tong University
  摘要：DNA aptamers that bind small molecules with high affinity have revolutionized the fields of biosensing and bioimaging. Recently, a DNA aptamer named 1301b has been identified as the most potent DNA aptamer for the binding of adenosine triphosphate (ATP) with a dissociation constant (K-D) of similar to 2.7 mu M. However, the structural basis and recognition mechanism remain unclear, hindering further development of this DNA aptamer. In this study, we first design a shortened DNA aptamer namely ...
• Logarithmic kinetics and bundling in random packings of elongated 3D physical links AI翻译
  作者：Bonamassa, Ivan; Rath, Balazs; Posfai, Marton; Abert, Miklos; Keliger, Daniel; Szegedy, Balazs; Kertesz, Janos; Lovasz, Laszlo; Barabasi, Albert-Laszlo
  作者单位：Budapest University of Technology & Economics; Northeastern University
  摘要：We explore the impact of excluded volume interactions on the local assembly of linear physical networks, where nodes are spheres and links are rigid cylinders with varying length. To focus on the effect of elongated links, we introduce a minimal 3D model that helps us zoom into confined regions of these networks whose distant parts are sequentially connected by the random deposition of physical links with a very large aspect ratio. We show that the nonequilibrium kinetics at which these elonga...
• Role of wettability, adhesion, and instabilities in transitions during lubricated sliding friction AI翻译
  作者：Dong, Hao; Siddiquie, Reshma Y.; Xiao, Xuemei; Andrews, Michael; Bergman, Brian; Hui, Chung-Yuen; Jagota, Anand
  作者单位：Lehigh University; Lehigh University; Cornell University; Michelin
  摘要：Lubricated contacts in soft materials are important in various engineering systems and natural settings. Three major lubrication regimes are boundary (BL), mixed (ML), and elastohydrodynamic (EHL) lubrication, where the contact region is dry, partially wetted, or fully wetted, respectively. The transition between these regimes is insufficiently understood, especially for soft contacts, which impedes desired control of lubricated sliding friction. Here, we report on the role of solid wettabilit...
• Spatiotemporal distribution of the glycoprotein pherophorin II reveals stochastic geometry of the growing ECM of Volvox carteri AI翻译
  作者：von der Heyde, Benjamin; Srinivasan, Anand; Birwa, Sumit Kumar; von der Heyde, Eva Laura; Hoehn, Steph S. M. H.; Coldstein, Raymond E.; Hallmann, Armin
  作者单位：University of Bielefeld; University of Cambridge
  摘要：The evolution of multicellularity involved the transformation of a simple cell wall of unicellular ancestors into a complex, multifunctional extracellular matrix (ECM). A suitable model organism to study the formation and expansion of an ECM during ontogenesis is the multicellular green alga Volvox carteri, which, along with the related volvocine algae, produces a complex, self-organized ECM composed of multiple substructures. These self-assembled structures primarily consist of hydroxyproline...
• Targeted deletions of large syntenic regions in Arabidopsis thaliana AI翻译
  作者：Papikian, Ashot; Rattner, Rachel J.; Kao, Jenni; Hauser, Neil; Allsing, Nicholas; Mamerto, Allen; Hartwick, Nolan T.; Colt, Kelly; Michael, Todd P.
  作者单位：Salk Institute; University of California System; University of California San Diego; University of California System; University of California San Diego
  摘要：Plant genomes have undergone multiple rounds of whole-genome duplication (WGD) throughout their evolutionary history. As a result, many species, including Arabidopsis thaliana, retain duplicated genomic segments, or syntenic regions, which harbor large numbers of paralogous genes preserved from these ancient WGD events. We deleted four large, duplicated blocks, ranging from similar to 115 kb to similar to 684 kb using Staphylococcus aureus Cas9 to explore the effects of knocking out these bloc...
• Deficiency in transmitter release triggers homeostatic transcriptional changes that increase presynaptic excitability AI翻译
  作者：Cypranowska, Caroline A.; Feldthouse, Maya; Choi, Yoon Gi; Bakshinska, Dariya; Li, Rachel; Newman, Zachary L.; Isacoff, Ehud Y.
  作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; Francis Crick Institute; University of California System; University of California San Diego; University of Minnesota System; University of Minnesota Twin Cities
  摘要：Weakening of synaptic transmission at the Drosophila larval neuromuscular junction triggers two forms of homeostatic compensation, one that increases the probability of glutamate release per action potential (Pr) and another that increases motoneuron (MN) activity. We investigated the molecular changes in MNs that underlie the increase in MN activity. RNA sequencing (RNA-seq) analysis on MNs whose glutamate release is weakened by knockdown of components of the MN transmitter release machinery ...
• Synthesis of large single- transcript pathways from oligonucleotide pools: Design of STARBURST, an autobioluminescent reporter AI翻译
  作者：Dvir, Gony; Xing, Zenan; Beldman, Irina; Rivera, Andres; Wheeldon, Ian; Cutler, Sean R.
  作者单位：University of California System; University of California Riverside; University of California System; University of California Riverside; University of California System; University of California Riverside; Universidad Nacional Autonoma de Mexico; University of California System; University of California Riverside; University of California System; University of California Riverside
  摘要：Methods for fast and inexpensive gene synthesis from oligonucleotide pools enable rapid iteration of genetic designs. Here, we describe iggypop (indexed Golden Gate gene assembly from PCR- amplified oligonucleotide pools), a simple computational-experimental pipeline that allows for low- cost design and synthesis of hundreds of genes from oligonucleotide pools using Golden Gate assembly methods. We used iggypop to synthesize a series of single- transcript autonomously bioluminescent reporters ...
• Casimir self-assembly: A platform for measuring nanoscale surface interactions in liquids AI翻译
  作者：Hoskova, Michaela; Kotov, Oleg, V; Kucukoz, Betul; Murphyc, Catherine J.; Shegaia, Timur O.
  作者单位：Chalmers University of Technology; Brno University of Technology; University of Illinois System; University of Illinois Urbana-Champaign
  摘要：Self-assembly (SA) plays a pivotal role in nanotechnology, offering cost-effective methods for bottom-up fabrication and providing versatile model systems for investigating fundamental interactions in various bioinspired systems. However, current methods for investigating and quantifying the dynamics of SA systems are limited in their applicability to planar interfaces, particularly in liquid environments. These methods typically rely on analyzing the collective behavior of particle suspension...
• Serum metabolic patterns reveal the diagnostic and prognostic role of alanine abnormality in ocular adnexal lymphoma AI翻译
  作者：Huang, Yida; Lin, Huimin; Shi, Jiahao; Chen, Yifan; Sang, Qi; Wang, Ruimin; Liu, Wanshan; Wu, Jiao; Li, Yanyan; Xu, Xiaoyu; Ding, Chunmeng; Yang, Shouzhi; Zhang, Juxiang; Jia, Renbing; Fan, Xianqun; Qian, Kun; Zhou, Yixiong
  作者单位：Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University
  摘要：Ocular adnexal lymphoma (OAL) is the most common orbital malignancy in adults. Advanced tools for precise diagnosis and prognosis of OAL are in demand. Here, the nanoparticle-enhanced laser desorption/ionization mass spectrometry was applied for the construction of OAL-associated serum metabolic patterns (SMPs) from 239 participants (104 OAL and 135 non-OAL). Through machine learning, the diagnostic performance with an area-under-the-curve (AUC) of 0.901 was achieved for OAL based on SMPs. Fur...