46期

最新文章

• Spatial variation in the mutation rate within the plant shoot apical meristem AI翻译
  作者：Amundson, Kirk R.; Marimuthu, Mohan P. A.; Nguyen, Oanh; Sarika, Konsam; DeMarco, Isabelle J.; Phan, Angelina; Henry, Isabelle M.; Comai, Luca
  作者单位：University of California System; University of California Davis; University of California System; University of California Davis; University of Massachusetts System; University of Massachusetts Amherst; Indian Council of Agricultural Research (ICAR)
  摘要：The potential impact of new mutations on organismal function and evolution depends on the developmental fate of the affected cells. Stem cells in the shoot apical meristem form much of the plant body, but only those in the L2 can form gametes. This raises the question of whether the mutation rate is optimized for this developmental plan. To test this, we compare stem cell mutations accumulated over decades of clonal propagation in two potato varieties with those arising during leaf-cell regene...
• Physiologically based pharmacokinetic model for CAR-T cell delivery and efficacy in solid tumors AI翻译
  作者：Hadjigeorgiou, Andreas G.; Munn, Lance L.; Stylianopoulos, Triantafyllos; Jain, Rakesh K.
  作者单位：University of Cyprus; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School
  摘要：Abnormal blood vessels limit the delivery and function of endogenous T cells as well as adoptively transferred Chimeric Antigen Receptor (CAR)-T cells in tumor microenvironment (TME). We recently showed that vascular normalization using anti-VEGF therapy can overcome these challenges and improve the outcome of CAR-T therapy in glioblastoma models in mice. Here, we developed a physiologically based pharmacokinetic model to simulate the dynamics of both adoptively transferred CAR-T cells and end...
• miR444b.2-HsfA1-AOC1 module mediates heat priming-enhanced blast resistance in rice (vol. 122, e2505764122, 2025) AI翻译
  作者：Qiu, Jiehua; Cao, Xiuxiu; Shi, Huanbin; Chen, Zhengting; Zhang, Xiaoyu; Cao, Zijian; Huang, Dongmei; Wen, Hui; Chen, Ya; Kou, Yanjun
• Wildfires have created instability within risk transfer markets. Here's a path forward AI翻译
  作者：Thompson, Matthew P.; Calkin, David E.; MacGregor, Donald M.; Young, Bryce A.; Barrett, Kimiko; Fischer, Erica C.; Johnson, Jesse, V
  作者单位：Oregon State University; University of Montana System; University of Montana
• A unified framework for identification of cell-type-specific spatially variable genes in spatial transcriptomic studies AI翻译
  作者：Wang, Zhiwei; Zeng, Yeqin; Tan, Ziyue; Chen, Yuheng; Huang, Xinrui; Zhao, Hongyu; Lin, Zhixiang; Yang, Can
  作者单位：Hong Kong University of Science & Technology; Yale University; Chinese University of Hong Kong; CUHK Shenzhen Research Institute; The Chinese University of Hong Kong, Shenzhen; Hong Kong University of Science & Technology; Hong Kong University of Science & Technology; Hong Kong University of Science & Technology
  摘要：Characterizing cell-type-specific spatially variable genes (SVGs) within tissue context is essential for exploring the landscape of complex biological systems in spatial transcriptomic (ST) studies. In this paper, we present a unified framework, the Mixture of Mixed Models (MMM), designed to directly model RNA count data and identify cell-type-specific SVGs while accounting for cell type composition and correcting for platform effects. Through a comprehensive simulation study and the analyses ...
• Auxin transport positions stem cells in the vascular cambium during normal development and regeneration AI翻译
  作者：Zhang, Xixi; Wang, Xiaoyu; Smetana, Ondrej; Makela, Meeri; Makila, Riikka; Scarpella, Enrico; Zhang, Jing; Mahonen, Ari Pekka
  作者单位：University of Helsinki; University of Helsinki; Nankai University; University of Alberta
  摘要：The vascular cambium contains bifacial stem cells producing secondary phloem in one and secondary xylem to the opposite direction. In Arabidopsis roots, these stem cells are first established adjacent to primary xylem cells suggesting that undifferentiated primary xylem cells function as stem-cell organizers. Here, we show that auxin transport-guided auxin accumulation in the cells with primary xylem identity (PXI cells) within the root apical meristem defines the vascular cambial stem-cell or...
• Early female germline development in Xenopus laevis: Stem cells, nurse cells, and germline cysts AI翻译
  作者：Davidian, Asya; Spradling, Allan C.
  作者单位：Howard Hughes Medical Institute; Carnegie Institution for Science
  摘要：In Drosophila, germline cysts arise through synchronous mitotic divisions and acquire a polarized architecture organized by the fusome, which guides oocyte specification and supports meiotic progression. Similar cyst structures exist in nonmammalian vertebrate ovaries, but their polarity and function have remained uncertain. Using single-cell RNA sequencing and high-resolution imaging, we reconstructed the germ cell differentiation trajectory in Xenopus laevis and uncovered striking parallels ...
• Fluorescein- based dyes are not valid reporters of oxidative stress in bacteria, and conclusions based on their use must be reconsidered AI翻译
  作者：Korshunov, Sergey; Imlay, James A.
  作者单位：University of Illinois System; University of Illinois Urbana-Champaign
  摘要：Dihydrodichlorofluorescein derivatives have been employed as reporters of intracellular reactive oxygen species (ROS) in innumerable studies. Their use in bacteria has led investigators to conclude that a wide array of cellular stresses owe their toxicity to the formation of ROS. However, in the present study we find that this dye reacts too poorly with physiological concentrations of superoxide or hydrogen peroxide to signal their presence, and that lethal doses of hydroxyl radicals are dispe...
• Diffuse pacemaker mechanism with distinctive organization drives pulsation in the octocoral Xenia umbellata AI翻译
  作者：Nadir, Elinor; Benayahu, Yehuda; Lalzar, Maya Ofek-; Gabrieli, Tslil; Lotan, Tamar
  作者单位：Tel Aviv University; University of Haifa; University of Haifa
  摘要：The unique, hypnotic pulsation behavior of certain soft corals of the Xeniidae family, the result of rhythmic opening and closing of their tentacles, has fascinated scientists since the 18th century. Repetitive motion is regulated by autonomous neural circuits known as pacemakers or central pattern generators. However, little is known about such circuits in nonbilaterian organisms like corals. In this report, Xenia umbellata, a fast-growing octocoral, served as a model organism to study the mu...
• Structural dynamics and neural representation of wing deformation AI翻译
  作者：Yarger, Alexandra M.; Maeda, Masateru; Siwanowicz, Igor; Kajiyama, Haruhiro; Walker, Simon M.; Bomphrey, Richard J.; Lin, Huai-Ti
  作者单位：Imperial College London; Marine Biological Laboratory - Woods Hole; University of London; University of London Royal Veterinary College; University of Leeds
  摘要：Locomotor control is facilitated by mechanosensory inputs that report how the body interacts with a physical medium. Effective representation of compliant wing deformations is particularly challenging due to the many degrees of freedom. Structural configurations can constrain the stimulus space, and strategic placement of sensors can simplify computation. Here, we measured and modeled wing displacement fields and characterized spatiotemporal encoding of the wing mechanosensors. Our data show h...