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作者：Ying, Nicole Ng Shu; Lim, Qin Ying; Yamada, Gen; Hirashima, Tsuyoshi
作者单位：National University of Singapore; National University of Singapore; Kyoto University
摘要：Biological ducts must transport fluids while preserving structural integrity, yet how mechano-signaling coordinates wall deformation with luminal flow in vivo remains unclear. Here, we combine intravital two-photon excitation microscopy, light-sheet imaging, and F & ouml;rster resonance energy transfer-based kinase biosensors to resolve ejaculation-like events in the mouse vas deferens. Acute phenylephrine stimulation elicits a sequence of luminal dynamics: an initial retrograde pressure-redis...
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作者：Ashraf, Md Arsalan; Singha, Tapas; Pradhan, Susav; David, Serene Rose; Sens, Pierre; Pullarkat, Pramod
作者单位：Department of Science & Technology (India); Raman Research Institute (RRI); Centre National de la Recherche Scientifique (CNRS); Sorbonne Universite; UNICANCER; Universite PSL; CNRS - Institute of Chemistry (INC); Institut Curie
摘要：The ability to generate active stresses within filamentous actin matrices is a fundamental and evolutionarily conserved process driving locomotion and morphogenetic changes in cells. The generation of pushing forces by actin polymerization is reasonably well understood, and is known to drive lamellipodia based motility and filopodial extension. Actin filaments decorated with myosin motors can also generate contractile stresses as in the cell cortex or in cytokinetic rings. In this article we u...
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作者：Chen, Yang; Wen, Junyan; He, Ze-Xu; Fan, Jing-Wei; Pan, Xin-Yu; Ji, Cheng; Gou, Huiyang; Yu, Xiaohui; Chen, Liucheng; Liu, Gang-Qin
作者单位：Chinese Academy of Sciences; Nanjing Institute of Geology & Paleontology, CAS; Institute of Physics, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Hefei University of Technology
摘要：Lanthanum hydride has attracted significant attention in recent years due to its signatures of superconductivity at around 250 K. However, the required megabar pressures present extraordinary challenges in verifying its Meissner effect. Although nitrogen-vacancy (NV) centers in diamond offer a solution, their application has been limited by insufficient working pressures. In this work, using a gaseous pressure-transmitting medium, the working pressure of NV centers is extended to nearly 180 GP...
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作者：Klein, Samuel A. W.; Charlesworth, Tessa E. S.; Heck, Daniel W.; Banaji, Mahzarin R.; Sherman, Jeffrey W.
作者单位：Columbia University; Northwestern University; Philipps University Marburg; Harvard University; University of California System; University of California Davis
摘要：One of psychology's most ubiquitous findings is that of robust ingroup preference, an explicitly stated human preference for the groups to which we belong. The advent of implicit measures of social cognition introduced a paradoxical result: Compared to members of higher-status groups, members of lower-status groups often show no implicit ingroup preference, sometimes even showing outgroup preference, a result rarely observed on explicit measures. Prevailing theories have provided macro-level a...
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作者：Rath, Sneha P.; Li, Zhu; Guha, Arkajit; Dong, Fangcong; Banerjee, Ruma; Mootha, Vamsi K.
作者单位：Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School; University of Michigan System; University of Michigan
摘要：Of the similar to 1,100 mitochondrial proteins, only a handful like PINK1 and ATFS-1 are known to stabilize and relocalize upon collapse of the proton motive force (PMF) to execute signaling roles. To systematically identify genes that increase exclusively at the protein level upon PMF collapse, we performed a joint proteomic and RNA-seq screen. The screen revealed 10 candidates (six mitochondrial), including two genes in vitamin B-12 metabolism -the B-12 chaperone MMADHC and cytosolic B-12-de...
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作者：Swanson, Larry W.; Hahn, Joel D.; Sporns, Olaf
作者单位：Indiana University System; Pennsylvania State System of Higher Education (PASSHE); Indiana University Bloomington; Indiana University of Pennsylvania
摘要：A consensus description of global vertebrate nervous system organization-its basic plan or wiring diagram-has not emerged after centuries of research. Our approach to the problem is based on published experimental neuroanatomical connection data that describe the adult female and male rat nervous system's intrinsic neuronal network organization at the interregional level of scale that is inherited and species specific. This bilateral network has 924 region nodes with a projected 81,582 directe...
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作者：Echeverria-Alar, Sebastian; Narasimhan, Badri Narayanan; Fraley, Stephanie I.; Rappel, Wouter-Jan
作者单位：University of California System; University of California San Diego; University of California System; University of California San Diego
摘要：Single cells confined by the extracellular matrix can exhibit rotational motion, yet the physical mechanisms underlying its onset and persistence remain unclear. Here, we address this gap with a cellular phase field model that couples cell deformation, cell polarization governed by stochastic excitable dynamics, and confinement. We identify the confinement strength as a bifurcation parameter determining three regimes: Strong confinement prevents rotation through spatial constraints, intermedia...
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作者：Kumar Varadwaj, Pritish
作者单位：Indian Institute of Information Technology Allahabad
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作者：Santos, Renato E. R. S.; Basu, Pallabi; O'Hara, Brendan J.; Gibson, Jacob A.; Gebhardt, Michael J.; Akut, Chris; Robins, William P.; Mekalanos, John J.; Dove, Simon L.; Watnick, Paula I.
作者单位：Harvard University; Harvard Medical School; Harvard University; Harvard Medical School; University of Iowa; Harvard University; Harvard Medical School
摘要：The cyclic AMP receptor protein or CRP is a global regulator of bacterial metabolism. CRP activates transcription of genes required for utilization of alternative carbon sources in response to the second messenger cAMP, which is synthesized in the setting of glucose scarcity. Transcription is activated through contact with RNA polymerase at three sites termed activating regions (ARs) 1-3. AR3 was previously reported to be functional only when CRP K52 was mutated to a neutral residue and to be ...
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作者：Zhang, Jia Yi; Dregni, Aurelio J.; Petkowski, Janusz J.; Seager, Sara; Hong, Mei
作者单位：Massachusetts Institute of Technology (MIT); Wroclaw University of Science & Technology; Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT)
摘要：The search for life beyond Earth has focused on planets and moons with liquid water, reflecting the assumption that complex biomolecules require aqueous environments to remain stable and functional. Such a view excludes a wide class of planetary settings, including the concentrated sulfuric acid clouds of Venus, where extreme acidity and minimal water are thought to preclude molecular structure despite suitable temperatures. A growing body of evidence shows that a wide range of organic molecul...
