31期

最新文章

• GABAA receptor subunit composition regulates circadian rhythms in rest-wake and synchrony among cells in the suprachiasmatic nucleus AI翻译
  作者：Granados-Fuentes, Daniel; Lambert, Peter; Simon, Tatiana; Mennerick, Steven; Herzog, Erik D.
  作者单位：Washington University (WUSTL); Washington University (WUSTL)
  摘要：The mammalian circadian clock located in the suprachiasmatic nucleus (SCN) produces robust daily rhythms including rest-wake. SCN neurons synthesize and respond to gamma- aminobutyric acid (GABA), but its role remains unresolved. We tested the hypothesis that gamma 2- and delta- subunits of the GABAA receptor in the SCN differ in their regulation of synchrony among circadian cells. We used two approaches: 1) shRNA to knock- down (KD) the expression of either gamma 2 or delta subunits in the SC...
• An ankyrin G-binding motif mediates TRAAK periodic localization at axon initial segments of hippocampal pyramidal neurons AI翻译
  作者：Luque-Fernandez, Virginia; Vanspauwen, Sam K.; Landra-Willm, Arnaud; Arvedsen, Emil; Besquent, Mailys; Sandoz, Guillaume; Rasmussen, Hanne B.
  作者单位：University of Copenhagen; Universite Cote d'Azur; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); CHU Nice; Universite Cote d'Azur
  摘要：The axon initial segment (AIS) is a critical compartment in neurons. It converts post- synaptic input into action potentials that subsequently trigger information transfer to target neurons. This process relies on the presence of several voltage- gated sodium (NaV) and potassium (KV) channels that accumulate in high densities at the AIS. TRAAK is a mechanosensitive leak potassium channel that was recently localized to the nodes of Ranvier. Here, we uncover that TRAAK is also present in AISs of...
• Ammonia transporter RhBG initiates downstream signaling and functional responses by activating NFκB AI翻译
  作者：Mishra, Saurabh; Welch, Nicole; Singh, Shashi Shekhar; Singh, Khuraijam Dhanachandra; Bellar, Annette; Kumar, Avinash; Deutz, Lars N.; Hanlon, Maxmillian D.; Kant, Sashi; Dastidar, Sumitava; Patel, Hailee; Agrawal, Vandana; Attaway, Amy H.; Musich, Ryan; Stark, George R.; Tedesco, Francesco Saverio; Truskey, George A.; Weiner, I. David; Karnik, Sadashiva S.; Dasarathy, Srinivasan
  作者单位：Cleveland Clinic Foundation; Cleveland Clinic Foundation; Cleveland Clinic Foundation; University of London; University College London; Francis Crick Institute; Duke University; Cleveland Clinic Foundation; Cleveland Clinic Foundation; State University System of Florida; University of Florida
  摘要：Transceptors, solute transporters that facilitate intracellular entry of molecules and also initiate intracellular signaling events, have been primarily studied in lower- order species. Ammonia, a cytotoxic endogenous metabolite, is converted to urea in hepatocytes for urinary excretion in mammals. During hyperammonemia, when hepatic metabolism is impaired, nonureagenic ammonia disposal occurs primarily in skeletal muscle. Increased ammonia uptake in skeletal muscle is mediated by a membrane- ...
• Disruption of the ZFP574-THAP12 complex suppresses B cell malignancies in mice AI翻译
  作者：Zhong, Xue; Moresco, James J.; Sorelle, Jeffrey A.; Song, Ran; Jiang, Yiao; Nguyen, Mylinh T.; Wang, Jianhui; Bu, Chun Hui; Moresco, Eva Marie Y.; Beutler, Bruce; Choi, Jin Huk
  作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center
  摘要：Despite the availability of life- extending treatments for B cell leukemias and lymphomas, many of these cancers remain incurable. Thus, the development of new molecular targets and therapeutics is needed to expand treatment options. To identify new molecular targets, we used a forward genetic screen in mice to identify genes required for development or survival of lymphocytes. Here, we describe Zfp574, essential gene encoding a zinc finger protein necessary for normal and malignant lymphocyte...
• Unified understanding of the impact of semiflexibility, concentration, and molecular weight on macromolecular-scale diffusion AI翻译
  作者：Mei, Baicheng; Grest, Gary S.; Liu, Songyue; O'Connor, Thomas C.; Schweizer, Kenneth S.
  作者单位：University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; United States Department of Energy (DOE); Sandia National Laboratories; Carnegie Mellon University; University of Illinois System; University of Illinois Urbana-Champaign
  摘要：Conformationally fluctuating, globally compact macromolecules such as polymeric rings, single- chain nanoparticles, microgels, and many- arm stars display complex dynamic behaviors due to their rich topological structure and intermolecular organization. Synthetic rings are hybrid objects with conformations that display both ideal random walk and compact globular features, which can serve as models of genomic DNA. To date, emphasis has been placed on the effect of ring molecular weight on their...
• Ethics and responsibility in biohybrid robotics research AI翻译
  作者：Mestre, Rafael; Astobiza, Anibal M.; Webster-Wood, Victoria A.; Ryan, Matt; Saif, M. Taher A.
  作者单位：University of Southampton; University of Southampton; University of Southampton; University of Southampton; University of Southampton; University of Basque Country; Carnegie Mellon University; Carnegie Mellon University; Carnegie Mellon University; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Goethe University Frankfurt; University of Illinois System; University of Illinois Urbana-Champaign
  摘要：The industrial revolution of the 19th century marked the onset of an era of machines and robots that transformed societies. Since the beginning of the 21st century, a new generation of robots envisions similar societal transformation. These robots are biohybrid: part living and part engineered. They may self-assemble and emerge from complex interactions between living cells. While this new era of living robots presents unprecedented opportunities for positive societal impact, it also poses a h...
• Reversing anxiety by targeting a stress- responsive signaling pathway AI翻译
  作者：Pandey, Saurabh; Han, Wenyan; Li, Jun; Shepard, Ryan; Wu, Kunwei; Castellano, David; Tian, Qingjun; Dong, Lijin; Li, Yan; Lu, Wei
  作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS); National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI); National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
  摘要：Current treatments of anxiety and depressive disorders are plagued by considerable side effects and limited efficacies, underscoring the need for additional molecular targets that can be leveraged to improve medications. Here, we have identified a molecular cascade triggered by chronic stress that exacerbates anxiety- and depressive- like behaviors. Specifically, chronic stress enhances Src kinase activity and tyrosine phosphorylation of resulting in decreased inhibitory transmission and heigh...
• Elucidating the roles of electrolytes and hydrogen bonding in the dewetting dynamics of the tear film AI翻译
  作者：Borkar, Suraj; Baumli, Philipp; Vance, Travis; Sharma, Ekta; Shi, Xinfeng; Wu, James Y.; Yao, George; Myung, David; Fuller, Gerald G.
  作者单位：Stanford University; Stanford University
  摘要：This study explores the impact of electrostatic interactions and hydrogen bonding on tear film stability, a crucial factor for ocular surface health. While mucosal and meibomian layers have been extensively studied, the role of electrolytes in the aqueous phase remains unclear. Dry eye syndrome, characterized by insufficient tear quantity or quality, is associated with hyperosmolality, making electrolyte composition an important factor that might impact tear stability. Using a model buffer sol...
• The CH1 domain influences the expression and antigen sensing of the HIV- specific CH31 IgM- BCR and IgG- BCR AI翻译
  作者：Ortiz, Yaneth; Anasti, Kara; Kane, Advaiti Pai; Cronin, Kenneth; Alam, S. Munir; Reth, Michael
  作者单位：University of Freiburg; University of Freiburg; University of Freiburg; Duke University; Duke University
  摘要：How different classes of the B cell antigen receptor (BCR) sense viral antigens used in vaccination protocols is poorly understood. Here, we study antigen binding and sensing of human Ramos B cells expressing a BCR of either the IgM or IgG1 class with specificity for the CD4- binding- site of the envelope (Env) protein of the HIV- 1. Both BCRs carry an identical antigen binding site derived from the broad neutralizing antibody (bnAb) CH31. We find a five times higher expression of the IgG1-BCR...
• Climate impacts of alternative beef production systems depend on the functional unit used: Weight or monetary value AI翻译
  作者：Wang, Tong; Kreuter, Urs; Davis, Christopher; Cheye, Stephen
  作者单位：South Dakota State University; Texas A&M University System; Texas A&M University College Station; United States Department of Agriculture (USDA)
  摘要：Beef production has been identified as a significant source of anthropogenic greenhouse gas (GHG) emissions in the agricultural sector. United States and Canada account for about a quarter of the world's beef supply. To compare the GHG emission contributions of alternative beef production systems, we conducted a meta- analysis of 32 studies that were conducted between 2001 and 2023. Results indicated that GHG emissions from beef production in North America varied almost fourfold from 10.2 to 3...