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作者：Riehl, Dennis R.; Sharma, Arjun; Roewe, Julian; Reinhardt, Christoph; Schaefer, Katrin; Bosmann, Markus
作者单位：Johannes Gutenberg University of Mainz; Boston University; Johannes Gutenberg University of Mainz; German Centre for Cardiovascular Research; Johannes Gutenberg University of Mainz; Johannes Gutenberg University of Mainz
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作者：Qu, Zhiyu; Xu, Lianjie; Jiang, Fengyi; Liu, Yuan; Zhang, Wen - Bin
作者单位：Peking University; Chinese Academy of Sciences; Peking University; Peking University Shenzhen Graduate School (PKU Shenzhen); Peking University
摘要：Expanding the protein fold space beyond linear chains is of fundamental significance, yet remains largely unexplored. Herein, we report the creation of seven topological isoforms (i.e., linear, cyclic, knot, lasso, pseudorotaxane, and catenane) from a single protein fold precursor by rewiring the connectivity of secondary structure elements of the SpyTag- SpyCatcher complex and mutating the reactive residue on SpyTag to abolish the isopeptide bonding. These topological isoforms can be directly...
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作者：Baldassarri, Ilaria; Tavakol, Daniel Naveed; Graney, Pamela L.; Chramiec, Alan G.; Hibshoosh, Hanina; Vunjak-Novakovic, Gordana
作者单位：Columbia University; Columbia University; Columbia University; Columbia University; Columbia University
摘要：Incomplete understanding of metastatic disease mechanisms continues to hinder effective treatment of cancer. Despite remarkable advancements toward the identification of druggable targets, treatment options for patients in remission following primary tumor resection remain limited. Bioengineered human tissue models of metastatic capable of recreating the physiologically relevant milieu of metastatic colonization strengthen our grasp of cancer progression and contribute to the development of ef...
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作者：Muto, Yoshiharu; Yoshimura, Yasuhiro; Wu, Haojia; Panesso, Monica Chang -; Ledru, Nicolas; Woodward, Owen M.; Outeda, Patricia; Cheng, Tao; Mahjoub, Moe R.; Watnick, Terry J.; Humphreys, Benjamin D.
作者单位：Washington University (WUSTL); University System of Maryland; University of Maryland Baltimore; University System of Maryland; University of Maryland Baltimore; Washington University (WUSTL); Washington University (WUSTL)
摘要：Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary kidney disease and causes significant morbidity, ultimately leading to kidney failure. PKD pathogenesis is characterized by complex and dynamic alterations in multiple cell types during disease progression, hampering a deeper understanding of disease mechanism and the development of therapeutic approaches. Here, we generate a single- nucleus multimodal atlas of an orthologous mouse PKD model at early, mid, and l...
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作者：Harold, Rachel L.; Tulsian, Nikhil K.; Narasimamurthy, Rajesh; Yaitanes, Noelle; Hernandez, Maria G. Ayala; Lee, Hsiau-Wei; Crosby, Priya; Tripathi, Sarvind M.; Virshup, David M.; Partch, Carrie L.
作者单位：University of California System; University of California Santa Cruz; National University of Singapore; National University of Singapore; Duke University; University of California System; University of California San Diego; Howard Hughes Medical Institute; University of California System; University of California Santa Cruz
摘要：Casein kinase 1 delta (CK1 delta) controls essential biological processes including circadian rhythms and wingless- related integration site (Wnt) signaling, but how its activity is regulated is not well understood. CK1 delta is inhibited by autophosphorylation of its intrinsically disordered C- terminal tail. Two CK1 splice variants, delta 1 and delta 2, are known to have very different effects on circadian rhythms. These variants differ only in the last 16 residues of the tail, referred to a...
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作者：Koster, Anna K.; Yarishkin, Oleg; Dubin, Adrienne E.; Kefauver, Jennifer M.; Pak, Ryan A.; Cravatt, Benjamin F.; Patapoutian, Ardem
作者单位：Howard Hughes Medical Institute; Scripps Research Institute; Scripps Research Institute; Scripps Research Institute
摘要：The propeller- shaped blades of the PIEZO1 and PIEZO2 ion channels partition into the plasma membrane and respond to indentation or stretching of the lipid bilayer, thus converting mechanical forces into signals that can be interpreted by cells, in the form of calcium flux and changes in membrane potential. While PIEZO channels participate in diverse physiological processes, from sensing the shear stress of blood flow in the vasculature to detecting touch through mechanoreceptors in the skin, ...
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作者：Shahriar, Imrul; Kamra, Mohini; Kanduluru, Ananda Kumar; Campbell, Charity Lynn; Nguyen, Thanh Hiep; Srinivasarao, Madduri; Low, Philip S.
作者单位：Purdue University System; Purdue University; Purdue University System; Purdue University; University of Texas System; University of Texas Austin
摘要：Despite much research, considerable data suggest that influenza virus remains a serious health problem because i) the effectiveness of current vaccines ranges only from 19% to 60%, ii) available therapies remain ineffective in advanced stages of disease, iii) death rates vary between 25,000 and 72,000/year in the United States, and iv) avian influenza strains are now being transmitted to dairy cattle that in turn are infecting humans. To address these concerns, we have developed zanDR, a bispe...
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作者：Jiang, Yong; Wu, Gaoying; Pu, Ying; Wang, Yue; Chu, Na; Zeng, Raymond Jianxiong; Zhang, Xudong; Zhu, Xiangdong; Liang, Peng
作者单位：Fujian Agriculture & Forestry University; Chinese Academy of Sciences; Chengdu Institute of Biology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Vanderbilt University; Chinese Academy of Sciences; Nanjing Institute of Soil Science, CAS; Tsinghua University
摘要：Acidic CO2 electrolysis, enhanced by the introduction of alkali cations, presents a strategic approach for improving carbon efficiency compared to processes conducted in neutral and alkaline environments. However, a significant challenge arises from the dissolution of both organic acids and alkali cations in a strongly acidic feed stream, resulting in a considerable energy penalty for downstream separation. In this study, we investigate the feasibility of using flow-electrode capacitive deioni...
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作者：Busoms, Silvia; da Silva, Ana C.; Escola, Gloria; Abdilzadeh, Raziyeh; Curran, Emma; Bollmann-Giolai, Anita; Bray, Sian; Wilson, Michael; Poschenrieder, Charlotte; Yant, Levi
作者单位：Autonomous University of Barcelona; University of Nottingham; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); John Innes Center; University of Nottingham; Charles University Prague; University of Cambridge; Max Planck Society
摘要：It is normally supposed that populations of the same species should evolve shared mechanisms of adaptation to common stressors due to evolutionary constraint. Here, we describe a system of within- species local adaptation to coastal habitats, Brassica fruticulosa, and detail surprising strategic variability in adaptive responses to high salinity. These different adaptive responses in neighboring populations are evidenced by transcriptomes, diverse physiological outputs, and distinct genomic se...
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作者：Chai, Liraz; Zaburdaev, Vasily; Kolter, Roberto
作者单位：Hebrew University of Jerusalem; Hebrew University of Jerusalem; Queensland University of Technology (QUT); University of Erlangen Nuremberg; Harvard University; Harvard Medical School
摘要：Modern molecular microbiology elucidates the organizational principles of bacterial biofilms via detailed examination of the interplay between signaling and gene regulation. A complementary biophysical approach studies the mesoscopic dependencies at the cellular and multicellular levels with a distinct focus on intercellular forces and mechanical properties of whole biofilms. Here, motivated by recent advances in biofilm research and in other, seemingly unrelated fields of biology and physics,...
