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作者：Fan, Lijuan; Liu, Bingnan; Wang, Youxia; Tang, Bin; Xu, Tianqi; Fu, Jian; Wang, Chuanlong; Liu, Yuan; Ge, Liangpeng; Wei, Hong; Ren, Wenkai
作者单位：South China Agricultural University; Chongqing University; Yangzhou University; Chongqing Academy of Animal Sciences; Huazhong Agricultural University; Huazhong Agricultural University
摘要：Gut microbiota plays a vital role in host metabolism; however, the influence of gut microbes on polyamine metabolism is unknown. Here, we found germ- free models possess elevated polyamine levels in the colon. Mechanistically, intestinal Lactobacillus murinus- derived small RNAs in extracellular vesicles down- regulate host polyamine metabolism by targeting the expression of enzymes in polyamine metabolism. In addition, Lactobacillus murinus delays recovery of dextran sodium sulfate- induced c...
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作者：Wang, Yujue; Xu, Youwei; Wang, Yue; Zhang, Jie; Chen, Lan; He, Xinheng; Fan, Wenjia; Wu, Kai; Hu, Wen; Cheng, Xi; Yang, Guizhu; Xu, H. Eric; Zhuang, Youwen; Sun, Shuyang
作者单位：Shanghai Jiao Tong University; Shanghai Jiao Tong University; Chinese Academy of Sciences; Shanghai Institute of Materia Medica, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Nanjing University of Chinese Medicine; Chinese Academy of Sciences; Shanghai Institute of Materia Medica, CAS; Shanghai Jiao Tong University
摘要：Somatostatin receptors (SSTRs) exert critical biological functions such as negatively regulating hormone release and cell proliferation, making them popular targets for developing therapeutics to treat endocrine disorders, especially neuroendocrine tumors. Although several panagonists mimicking the endogenous ligand somatostatin are available, the development of more effective and safer somatostatinergic therapies is limited due to a lack of molecular understanding of the ligand recognition an...
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作者：Han, Jian; Song, Yangyang; Xie, Jinghe; Tano, Vincent; Shen, Haoqing; Gan, Wei Liang; Ng, Larry; Ng, Bryan Yik Loong; Ng, Vanessa Hui En; Sui, Xiaohui; Tang, Sze Jing; Chen, Leilei
作者单位：National University of Singapore; National University of Singapore
摘要：N6- methyladenosine (m6A) RNA methylation is a prevalent RNA modification that significantly impacts RNA metabolism and cancer development. Maintaining the global m6A levels in cancer cells relies on RNA accessibility to methyltransferases and the availability of the methyl donor S- adenosylmethionine (SAM). Here, we reveal that death associated protein 3 (DAP3) plays a crucial role in preserving m6A levels through two distinct mechanisms. First, although DAP3 is not a component of the m6A wri...
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作者：Mendieta, John Pablo; Tu, Xiaoyu; Jiang, Daiquan; Yan, Haidong; Zhang, Xuan; Marand, Alexandre P.; Zhong, Silin; Schmitz, Robert J.
作者单位：University of Michigan System; University of Michigan; University System of Georgia; University of Georgia; Shanghai Jiao Tong University; Chinese University of Hong Kong
摘要：While considerable knowledge exists about the enzymes pivotal for C4 photosynthesis, much less is known about the cis- regulation important for specifying their expression in distinct cell types. Here, we use single- cell- indexed ATAC-seq to identify cell- type- specific accessible chromatin regions (ACRs) associated with C4 enzymes for five different grass species. This study spans four C4 species, covering three distinct photosynthetic subtypes: Zea mays and Sorghum bicolor (NADP- dependent...
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作者：Wang, Xiying; Geng, Ji; Rimal, Suman; Sui, Yuxiu; Pan, Jie; Qin, Zhenghong; Lu, Bingwei
作者单位：Nanjing Medical University; Stanford University; Soochow University - China; Soochow University - China
摘要：It is well established that DNA Damage Regulated Autophagy Modulator 1 (DRAM1), a lysosomal protein and a target of p53, participates in autophagy.The cellular functions of DRAM1 beyond autophagy remain elusive. Here, we show p53- dependent upregulation of DRAM1 in mitochondrial damage-induced Parkinson's disease (PD) models and exacerbation of disease phenotypes by DRAM1. We find that the lysosomal location of DRAM1 relies on its intact structure including the cytosol- facing C- terminal doma...
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作者：Sanchez, Juan; Dammann, Lars; Gallardo, Laura; Li, Zhuoqing; Froeba, Michael; Meissner, Robert H.; Stone, Howard A.; Huber, Patrick
作者单位：Hamburg University of Technology; Helmholtz Association; Deutsches Elektronen-Synchrotron (DESY); Hamburg University of Technology; University of Hamburg; University of Hamburg; Helmholtz Association; Helmholtz-Zentrum Hereon; Princeton University
摘要：Capillarity-driven transport in nanoporous solids is widespread in nature and crucial for modern liquid-infused engineering materials. During imbibition, curved menisci driven by high negative Laplace pressures exert an enormous contractile load on porous matrix. Due to the challenge of simultaneously monitoring imbibition and deformation with high spatial resolution, the resulting coupling of solid elasticity liquid capillarity has remained largely unexplored. Here, we study water imbibition ...
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作者：Hardcastle, Matthew; Chen, Zhijian (James)
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作者：Coates, Ian A.; Pan, William; Saccone, Max A.; Lipkowitz, Gabriel; Ilyin, Dan; Driskill, Madison M.; Dulay, Maria T.; Frank, Curtis W.; Shaqfeh, Eric S. G.; Desimone, Joseph M.
作者单位：Stanford University; Stanford University; Stanford University
摘要：Stereolithography enables the fabrication of three- dimensional (3D) freeform structures via light- induced polymerization. However, the accumulation of ultraviolet dose within resin trapped in negative spaces, such as microfluidic channels or voids, can result in the unintended closing, referred to as overcuring, of these negative spaces. We report the use of injection continuous liquid interface production to continuously displace resin at risk of overcuring in negative spaces created in pre...
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作者：Iida, Shiori; Ide, Satoru; Tamura, Sachiko; Sasai, Masaki; Tani, Tomomi; Goto, Tatsuhiko; Shribak, Michael; Maeshima, Kazuhiro
作者单位：Research Organization of Information & Systems (ROIS); National Institute of Genetics (NIG) - Japan; Graduate University for Advanced Studies - Japan; Kyoto University; Nagoya University; National Institute of Advanced Industrial Science & Technology (AIST); Obihiro University of Agriculture & Veterinary Medicine; Obihiro University of Agriculture & Veterinary Medicine; Marine Biological Laboratory - Woods Hole
摘要：Genomic information must be faithfully transmitted into two daughter cells during mitosis. To ensure the transmission process, interphase chromatin is further condensed into mitotic chromosomes. Although protein factors like condensins and topoisomerase II alpha are involved in the assembly of mitotic chromosomes, the physical bases of the condensation process remain unclear. Depletion attraction/macromolecular crowding, an effective attractive force that arises between large structures in cro...
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作者：Ioannou, Ion A.; Monck, Carolina; Ceroni, Francesca; Brooks, Nicholas J.; Kuimova, Marina K.; Elani, Yuval
作者单位：Imperial College London; Imperial College London; Imperial College London; Imperial College London
摘要：Eukaryotic cells are characterized by multiple chemically distinct compartments, one of the most notable being the nucleus. Within these compartments, there is a continuous exchange of information, chemicals, and signaling molecules, essential for coordinating and regulating cellular activities. One of the main goals of bottom-up synthetic biology is to enhance the complexity of synthetic cells by establishing functional compartmentalization. There is a need to mimic autonomous signaling betwe...
