2025

最新文章

• Highly spatial-temporal electrochemical profiling of molecules trafficking at a single mitochondrion in one living cell AI翻译
  作者：Liu, Kang; Wu, Lina; Ma, Yuanyuan; Chen, Desheng; Liu, Rujia; Zhang, Xiaobo; Jiang, Dechen; Pan, Rongrong
  作者单位：Nanjing University; Nanjing Normal University; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS
  摘要：Simultaneous profiling of multiple molecules trafficking at a single organelle and the surrounding cytosol within a living cell is crucial for elucidating their functions, necessitating advanced techniques that provide high spatial-temporal resolution and molecule specificity. In this study, we present an electrochemical nanodevice based on a 0-nanopipette designed to coanalyze calcium ions (Ca2+) and reactive oxygen species (ROS) at a single mitochondrion and its surrounding cytosol, thereby ...
• Characterization of diverse Cas9 orthologs for genome and epigenome editing AI翻译
  作者：Butterfield, Gabriel L.; Rohm, Dahlia; Roberts, Avery; Nethery, Matthew A.; Rizzo, Anthony J.; Morone, Daniel J.; Garnier, Lisa; Iglesias, Nahid; Barrangou, Rodolphe; Gersbach, Charles A.
  作者单位：Duke University; Duke University; North Carolina State University
  摘要：CRISPR-Cas9 systems have revolutionized biotechnology, creating diverse new opportunities for biomedical research and therapeutic genome and epigenome editing. Despite the abundance of bacterial CRISPR-Cas9 systems, relatively few are effective in human cells, limiting the overall potential of CRISPR technology. To expand the CRISPR-Cas toolbox, we characterized a set of type II CRISPR-Cas9 systems from select bacterial genera and species encoding diverse Cas9s. Four systems demonstrated robus...
• The Arabidopsis BUB1/MAD3 family protein BMF3 requires BUB3.3 to recruit CDC20 to kinetochores in spindle assembly checkpoint signaling (vol 121, e2322677121, 2024) AI翻译
  作者：Deng, Xingguang; Peng, Felicia Lei; Tang, Xiaoya; Lee, Yuh-Ru Julie; Lin, Hong-Hui; Liu, Bo
• Bacterial motility depends on a critical flagellum length and energy-optimized assembly AI翻译
  作者：Halte, Manuel; Popp, Philipp F.; Hathcock, David; Severn, John; Fischer, Svenja; Goosmann, Christian; Ducret, Adrien; Charpentier, Emmanuelle; Tu, Yuhai; Lauga, Eric; Erhardt, Marc; Renault, Thibaud T.
  作者单位：Humboldt University of Berlin; International Business Machines (IBM); IBM USA; University of Cambridge; Max Planck Society; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Universite Claude Bernard Lyon 1; Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Universite de Bordeaux
  摘要：The flagellum is the most complex macromolecular structure known in bacteria and is composed of around two dozen distinct proteins. The main building block of the long, external flagellar filament, flagellin, is secreted through the flagellar type-III secretion system at a remarkable rate of several tens of thousands of amino acids per second, significantly surpassing the rates achieved by other pore-based protein secretion systems. The evolutionary implications and potential benefits of this ...
• N6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins AI翻译
  作者：Wang, Qian; Xu, Shuai; Shen, Wentao; Wei, Yanli; Han, Lu; Wang, Zhengxiang; Yu, Yingying; Liu, Minxuan; Liu, Junwen; Deng, Guohua; Chen, Hualan; Zhu, Qiyun
  作者单位：Chinese Academy of Agricultural Sciences; Lanzhou Veterinary Research Institute, CAAS; Lanzhou University; Chinese Academy of Agricultural Sciences; Harbin Veterinary Research Institute, CAAS
  摘要：N6-methyladnosine (m6A) modification is present in both positive-and negative-strand RNA of influenza A virus (IAV) and affects the replication and pathogenicity of IAV. However, little is known about the regulatory mechanism of m6A in IAV RNA. In the present study, we identified the m6A methylation of the viral RNA of different IAV subtypes and confirmed that m6A modification promotes the polymerase activity and replication of IAV. By mutating m6A motifs on the multiple viral RNAs (vRNAs) of ...
• Brain- wide dynamic coactivation states code for hand movements in the resting state AI翻译
  作者：Zhang, Lu; Pini, Lorenzo; Shulman, Gordon L.; Corbetta, Maurizio
  作者单位：Ningbo University; University of Padua; Washington University (WUSTL); Washington University (WUSTL); University of Padua; Veneto Institute Molecular Medicine
  摘要：Resting brain activity, in the absence of explicit tasks, appears as distributed spatiotemporal patterns that reflect structural connectivity and correlate with behavioral traits. However, its role in shaping behavior remains unclear. Recent evidence shows that resting- state spatial patterns not only align with task- evoked topographies but also features, suggesting mechanisms for long- term storage and predictive coding. While prior research focused on static, time- averaged task activations...
• Rippled metamaterials with scale-dependent tailorable elasticity AI翻译
  作者：Zhou, Jian; Huang, Richard; Moldovan, Nicolaie; Stan, Liliana; Wen, Jianguo; Jin, Dafei; Nelson, David R.; Kosmrlj, Andrej; Czaplewski, David A.; Lopez, Daniel
  作者单位：United States Department of Energy (DOE); Argonne National Laboratory; State University of New York (SUNY) System; Binghamton University, SUNY; Harvard University; University of Notre Dame; Princeton University; Princeton University; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park
  摘要：Thermally induced ripples are intrinsic features of nanometer-thick films, atomically thin materials, and cell membranes, significantly affecting their elastic properties. Despite decades of theoretical studies on the mechanics of suspended thermalized sheets, controversy still exists over the impact of these ripples, with conflicting predictions about whether elasticity is scale-dependent or scale-independent. Experimental progress has been hindered so far by the inability to have a platform ...
• Record of paleo water divide locations reveals intermittent divide migration and links to paleoclimate proxies AI翻译
  作者：Harel, Elhanan; Goren, Liran; Crouvi, Onn; Porat, Naomi; Qu, Tianyue; Ginat, Hanan; Shelef, Eitan
  作者单位：Ben-Gurion University of the Negev; Geological Survey Israel; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh
  摘要：Drainage divide migration alters the geometry of drainage basins, influencing the distribution of water, erosion, sediments, and ecosystems across Earth's surface. The rate of divide migration is governed by differences in erosion rates across the divide and is thus sensitive to spatiotemporal variations in tectonics and climate. However, established approaches for quantifying divide migration rates offer only indirect evidence for the motion of the divide and provide only migration rate avera...
• G- quadruplexes and their unexpected ability to fold proteins AI翻译
  作者：Schwalbe, Harald; Burkhart, Ines
  作者单位：Goethe University Frankfurt
• Bipartite binding of the intrinsically disordered scaffold protein JIP1 to the kinase JNK1 AI翻译
  作者：Orand, Thibault; Delaforge, Elise; Lee, Alexandra; Kragelj, Jaka; Maud, Tengo; Tengo, Laura; Blackledge, Martin; Erba, Elisabetta Boeri; Davis, Roger JD.; Palencia, Andres; Jensen, Malene Ringkjobing
  作者单位：National Institute of Chemistry - Slovenia; European Molecular Biology Laboratory (EMBL); Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA); CEA; Centre National de la Recherche Scientifique (CNRS); University of Massachusetts System; University of Massachusetts Worcester; Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA)
  摘要：Scaffold proteins are key players in many signaling pathways where they ensure spatial and temporal control of molecular interactions by simultaneous tethering of multiple signaling components. The protein JIP1 acts as a scaffold within the c- Jun N- terminal kinase (JNK) signaling pathway by assembling three kinases, MLK3, MKK7, and JNK, into a macromolecular complex that enables their specific activation. The recruitment of these kinases depends on the 450- amino acid intrinsically disordere...