8019期

最新文章

• 3D model brains with cells from several people are first of their kind AI翻译
  作者：Mullard, Asher
• Atom-swap chemistry speeds synthesis of compounds for drug discovery AI翻译
  作者：Renteria-Gomez, Angel; Gutierrez, Osvaldo
  作者单位：Texas A&M University System; Texas A&M University College Station
• Research priorities for the next government AI翻译
  作者：[Anonymous]
  摘要：Ministers must take a global view of science, address a growing crisis in university finances and put a stop to interference in research autonomy.
• An alternative broad-specificity pathway for glycan breakdown in bacteria AI翻译
  作者：Nasseri, Seyed Amirhossein; Lazarski, Aleksander C.; Lemmer, Imke L.; Zhang, Chloe Y.; Brencher, Eva; Chen, Hong-Ming; Sim, Lyann; Panwar, Deepesh; Betschart, Leo; Worrall, Liam J.; Brumer, Harry; Strynadka, Natalie C. J.; Withers, Stephen G.
  作者单位：University of British Columbia; University of British Columbia; University of British Columbia; University of British Columbia
  摘要：The vast majority of glycosidases characterized to date follow one of the variations of the 'Koshland' mechanisms(1) to hydrolyse glycosidic bonds through substitution reactions. Here we describe a large-scale screen of a human gut microbiome metagenomic library using an assay that selectively identifies non-Koshland glycosidase activities(2). Using this, we identify a cluster of enzymes with extremely broad substrate specificities and thoroughly characterize these, mechanistically and structu...
• Most nearby young star clusters formed in three massive complexes AI翻译
  作者：Swiggum, Cameren; Alves, Joao; Benjamin, Robert; Ratzenboeck, Sebastian; Miret-Roig, Nuria; Grossschedl, Josefa; Meingast, Stefan; Goodman, Alyssa; Konietzka, Ralf; Zucker, Catherine; Hunt, Emily L.; Reffert, Sabine
  作者单位：University of Vienna; University of Wisconsin System; University of Vienna; University of Cologne; Smithsonian Institution; Ruprecht Karls University Heidelberg
  摘要：Efforts to unveil the structure of the local interstellar medium and its recent star-formation history have spanned the past 70 years (refs. 1-6). Recent studies using precise data from space astrometry missions have revealed nearby, newly formed star clusters with connected origins7-12. Nonetheless, mapping young clusters across the entire sky back to their natal regions has been hindered by a lack of clusters with precise radial-velocity data. Here we show that 155 out of 272 (57%) high-qual...
• High-resolution in situ structures of mammalian respiratory supercomplexes AI翻译
  作者：Zheng, Wan; Chai, Pengxin; Zhu, Jiapeng; Zhang, Kai
  作者单位：Nanjing University of Chinese Medicine; Yale University
  摘要：Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes(1-4). Despite extensive in vitro structural studies, determining the atomic details of their molecular mechanisms in physiological states remains a major challenge, primarily because of loss of the native environment during purification. Here we directly image porcine mitochondria using an in situ cryo-electron microscopy ...
• Expanding chemistry through in vitro and in vivo biocatalysis AI翻译
  作者：Kissman, Elijah N.; Sosa, Max B.; Millar, Douglas C.; Koleski, Edward J.; Thevasundaram, Kershanthen; Chang, Michelle C. Y.
  作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; Princeton University
  摘要：Living systems contain a vast network of metabolic reactions, providing a wealth of enzymes and cells as potential biocatalysts for chemical processes. The properties of protein and cell biocatalysts-high selectivity, the ability to control reaction sequence and operation in environmentally benign conditions-offer approaches to produce molecules at high efficiency while lowering the cost and environmental impact of industrial chemistry. Furthermore, biocatalysis offers the opportunity to gener...
• GETTING UNDER THE SKIN OF ROBOTS TO MAKE THEM SMILE AI翻译
  作者：[Anonymous]
• Structural basis for pegRNA-guided reverse transcription by a prime editor AI翻译
  作者：Shuto, Yutaro; Nakagawa, Ryoya; Zhu, Shiyou; Hoki, Mizuki; Omura, Satoshi N.; Hirano, Hisato; Itoh, Yuzuru; Zhang, Feng; Nureki, Osamu
  作者单位：University of Tokyo; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Howard Hughes Medical Institute
  摘要：The prime editor system composed of Streptococcus pyogenes Cas9 nickase (nSpCas9) and engineered Moloney murine leukaemia virus reverse transcriptase (M-MLV RT) collaborates with a prime editing guide RNA (pegRNA) to facilitate a wide variety of precise genome edits in living cells(1). However, owing to a lack of structural information, the molecular mechanism of pegRNA-guided reverse transcription by the prime editor remains poorly understood. Here we present cryo-electron microscopy structur...
• Streamlined collaboration can boost CRISPR gene therapies for rare diseases AI翻译
  作者：He, Wei; Zeng, Shuxiong; Xu, Chuanliang
  作者单位：Naval Medical University; Shanghai Jiao Tong University