Expanding chemistry through in vitro and in vivo biocatalysis

• 成果类型: Review
• 署名作者: Kissman, Elijah N.; Sosa, Max B.; Millar, Douglas C.; Koleski, Edward J.; Thevasundaram, Kershanthen; Chang, Michelle C. Y.
• 署名单位: University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; Princeton University
• 刊物名称: Nature
• ISSN/ISSBN: 0028-3812
• DOI: 10.1038/s41586-024-07506-w
• 发表日期: 2024-07-04
• 页码: 37-48
• 关键词: artificial metalloenzyme; directed evolution; chemoenzymatic synthesis; synthetic biology; enzyme; bond; cytochrome-p450; biosynthesis; systems; PATHWAY

摘要

Living systems contain a vast network of metabolic reactions, providing a wealth of enzymes and cells as potential biocatalysts for chemical processes. The properties of protein and cell biocatalysts-high selectivity, the ability to control reaction sequence and operation in environmentally benign conditions-offer approaches to produce molecules at high efficiency while lowering the cost and environmental impact of industrial chemistry. Furthermore, biocatalysis offers the opportunity to generate chemical structures and functions that may be inaccessible to chemical synthesis. Here we consider developments in enzymes, biosynthetic pathways and cellular engineering that enable their use in catalysis for new chemistry and beyond. This Review considers developments in enzymes, biosynthetic pathways and cellular engineering that enable their use in catalysis for new chemistry and beyond.