作者：Brodel, Andreas K.; Charpenay, Loic H.; Galtier, Matthieu; Fuche, Fabien J.; Terrasse, Remi; Poquet, Chloe; Havranek, Jan; Pignotti, Simone; Krawczyk, Antonina; Arraou, Marion; Prevot, Gautier; Spadoni, Dalila; Yarnall, Matthew T. N.; Hessel, Edith M.; Fernandez-Rodriguez, Jesus; Duportet, Xavier; Bikard, David
作者单位：Pasteur Network; Universite Paris Cite; Institut Pasteur Paris
摘要：Microbiome research is now demonstrating a growing number of bacterial strains and genes that affect our health1. Although CRISPR-derived tools have shown great success in editing disease-driving genes in human cells2, we currently lack the tools to achieve comparable success for bacterial targets in situ. Here we engineer a phage-derived particle to deliver a base editor and modify Escherichia coli colonizing the mouse gut. Editing of a beta-lactamase gene in a model E. coli strain resulted i...
作者：Wand, A. Joshua
作者单位：Texas A&M University System; Texas A&M University College Station
摘要：Proteins have been designed that assemble in different ways depending on whether an 'effector' molecule is present - a demonstration of allostery, the phenomenon that enables switch-like control of protein functions in nature. Designer proteins that exhibit the phenomenon of allostery.
