Proceedings of the National Academy of Sciences of the United States of America

最新文章

• Sustainable, low- cost, high- contrast electrochromic displays via host-guest interactions AI翻译
  作者：Wang, Yuyang; Lei, Chuxin; Guan, Weixin; Shi, Wen; Shen, Ruipeng; Zhang, Sean Xiao- An; Yu, Guihua
  作者单位：University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; Jilin University
  摘要：Electrochromic (EC) displays with electronically regulating the transmittance of solar radiation offer the opportunity to increase the energy efficiency of the building and electronic products and improve the comfort and lifestyle of people. Despite the unique merit and vast application potential of EC technologies, long - awaited EC windows and related visual content displays have not been fully commercialized due to unsatisfactory production cost, durability, color, and complex fabrication p...
• Structure and dynamics of a pentameric KCTD5/CUL3/Gβγ E3 ubiquitin ligase complex AI翻译
  作者：Nguyen, Duc Minh; Rath, Deanna H.; Devost, Dominic; Petrin, Darlaine; Rizk, Robert; Ji, Alan X.; Narayanan, Naveen; Yong, Darren; Zhai, Andrew; Kuntz, Douglas A.; Mian, Maha U. Q.; Pomroy, Neil C.; Keszei, Alexander F. A.; Benlekbir, Samir; Mazhab-Jafari, Mohammad T.; Rubinstein, John L.; Hebert, Terence E.; Prive, Gilbert G.
  作者单位：University of Toronto; University Health Network Toronto; Princess Margaret Cancer Centre; University of Toronto; McGill University; University of Toronto; Hospital for Sick Children (SickKids); University of Toronto
  摘要：Heterotrimeric G proteins can be regulated by posttranslational modifications, including ubiquitylation. KCTD5, a pentameric substrate receptor protein consisting of an N-terminal BTB domain and a C-terminal domain, engages CUL3 to form the central scaffold of a cullin-RING E3 ligase complex (CRL3(KCTD5)) that ubiquitylates G beta gamma and reduces G beta gamma protein levels in cells. The cryo-EM structure of a 5:5:5 KCTD5/CUL3(NTD)/G beta(1)gamma(2) assembly reveals a highly dynamic complex ...
• Engineering a nanoantibiotic system displaying dual mechanism of action AI翻译
  作者：Xing, Huihua; Pereira, Aramis Jose; Fiora, Maria Mercedes; Aguiar-Alves, Fabio; Tagliazucchi, Mario; Conda-Sheridan, Martin
  作者单位：University of Nebraska System; University of Nebraska Medical Center; University of Buenos Aires; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); University of Buenos Aires; Palm Beach Atlantic University West Palm Beach
  摘要：In recent decades, peptide amphiphiles (PAs) have established themselves as promising efficacy. We studied two strategies: PA-LJC nanostructures (Encapsulation) and PA nanostructures + free LJC (Combination). Computational modeling using a molecular theory for amphiphile self- assembly captures and explains the morphology of PA-LJC nanostructures and the location of encapsulated LJC in agreement with transmission electron microscopy and two- dimensional (2D) NMR observations. The morphology an...
• Polyphosphate affects cytoplasmic and chromosomal dynamics in nitrogen-starved Pseudomonas aeruginosa AI翻译
  作者：Magkiriadou, Sofia; Stepp, Willi L.; Newman, Dianne K.; Manley, Suliana; Racki, Lisa R.
  作者单位：Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; California Institute of Technology; California Institute of Technology; Scripps Research Institute
  摘要：Polyphosphate (polyP) synthesis is a ubiquitous stress and starvation response in bacteria. In diverse species, mutants unable to make polyP have a wide variety of physiological defects, but the mechanisms by which this simple polyanion exerts its effects remain unclear. One possibility is that polyP's many functions stem from global effects on the biophysical properties of the cell. We characterize the effect of polyphosphate on cytoplasmic mobility under nitrogen-starvation conditions in the...
• Unexpected omega-3 activities in intracellular lipolysis and macrophage foaming revealed by fluorescence lifetime imaging AI翻译
  作者：Tang, Hesen; Liu, Zhengjie; Han, Guangmei; Geng, Junlong; Liu, Bianhua; Zhang, Ruilong; Zhang, Zhongping
  作者单位：Anhui University; Chinese Academy of Sciences; Hefei Institutes of Physical Science, CAS
  摘要：Omega - 3 polyunsaturated fatty acids (PUFA) found primarily in fish oil have been a popular supplement for cardiovascular health because they can substantially reduce circulating triglyceride levels in the bloodstream to prevent atherosclerosis. Beyond this established extracellular activity, here, we report a mode of action of PUFA, regulating intracellular triglyceride metabolism and lipid droplet (LD) dynamics. Real - time imaging of the subtle and highly dynamic changes of intracellular l...
• The Titanic question in TB control: Should we worry about the bummock? AI翻译
  作者：Fortune, Sarah M.
  作者单位：Harvard University; Harvard T.H. Chan School of Public Health
• Defining T cell receptor repertoires using nanovial-based binding and functional screening AI翻译
  作者：Koo, Doyeon; Mao, Zhiyuan; Dimatteo, Robert; Noguchi, Miyako; Tsubamoto, Natalie; McLaughlin, Jami; Tran, Wendy; Lee, Sohyung; Cheng, Donghui; de Rutte, Joseph; Sojo, Giselle Burton; Witte, Owen N.; Di Carlo, Dino
  作者单位：University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; UCLA Jonsson Comprehensive Cancer Center; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; University of California Los Angeles Medical Center; David Geffen School of Medicine at UCLA; University of California System; University of California Los Angeles
  摘要：The ability to selectively bind to antigenic peptides and secrete effector molecules can define rare and low- affinity populations of cells with therapeutic potential in emerging T cell receptor (TCR) immunotherapies. We leverage cavity- containing hydrogel microparticles, called nanovials, each coated with peptide - major histocompatibility complex (pMHC) monomers to isolate antigen- reactive T cells. T cells are captured and activated by pMHCs inducing the secretion of effector molecules inc...
• Ligand- induced protein transition state stabilization switches the binding pathway from conformational selection to induced fit AI翻译
  作者：Stenstroem, Olof; Diehl, Carl; Modig, Kristofer; Akke, Mikael
  作者单位：Lund University
  摘要：Protein-ligand complex formation is fundamental to biological function. A central question is whether proteins spontaneously adopt binding- competent conformations to which ligands bind conformational selection (CS) or whether ligands induce the binding- competent conformation induced fit (IF). Here, we resolve the CS and IF binding pathways by characterizing protein conformational dynamics over a wide range of ligand concentrations using NMR relaxation dispersion. We determined the relative f...
• Costs of being a diet generalist for the protist predator Dictyostelium discoideum AI翻译
  作者：Shreenidhia, P. M.; Brocka, Debra A.; Mccabea, Rachel I.; Strassmanna, Joan E.; Quellera, David C.
  作者单位：Washington University (WUSTL)
  摘要：Consumers range from specialists that feed on few resources to generalists that feed on many. Generalism has the clear advantage of having more resources to exploit, but the costs that limit generalism are less clear. We explore two understudied costs of gener alism in a generalist amoeba predator, Dictyostelium discoideum, feeding on naturally co- occurring bacterial prey. Both involve costs of combining prey that are suitable on their own. First, amoebas exhibit a reduction in growth rate wh...
• Multiple retinal isomerizations during the early phase of the bestrhodopsin photoreaction AI翻译
  作者：Kaziannis, Spyridon; Broser, Matthias; Stokkum, Ivo H. M. van; Dostal, Jakub; Busse, Wayne; Munhoven, Arno; Bernardo, Cesar; Kloz, Miroslav; Hegemann, Peter; Kennis, John T. M.
  作者单位：University of Ioannina; Humboldt University of Berlin; Vrije Universiteit Amsterdam
  摘要：Bestrhodopsins constitute a class of light- regulated pentameric ion channels that consist of one or two rhodopsins in tandem fused with bestrophin ion channel domains. Here, we report on the isomerization dynamics in the rhodopsin tandem domains of Phaeocystis antarctica bestrhodopsin, which binds all - trans retinal Schiff - base (RSB) absorbing at 661 nm and, upon illumination, converts to the meta- stable P540 state with an unusual 11 - cis RSB. The primary photoproduct P682 corresponds to...