Proceedings of the National Academy of Sciences of the United States of America

最新文章

• Surface delivery quantification reveals distinct trafficking efficiencies among clustered protocadherin isoforms AI翻译
  作者：May, Elizabeth J.; Gaudet, Rachelle
  作者单位：Harvard University; University of California System; University of California Berkeley
  摘要：Proteins that transmit molecules and signals across the plasma membrane are crucial in cell biology because they enable cells to sense and respond to their surroundings. A major challenge for studying cell surface proteins is that often they do not fold or traffic properly to the plasma membrane when produced in heterologous cells. We developed a strategy for quantifying surface localization from fluorescence microscopy images of surface-stained cells. Using clustered protocadherins, a protein...
• Structural insights into a citrate transporter that mediates aluminum tolerance in barley AI翻译
  作者：Thao, Tran Nguyen; Mitani-Ueno, Namiki; Urano, Ryo; Saitoh, Yasunori; Wang, Peitong; Yamaji, Naoki; Shen, Jian-Ren; Shinoda, Wataru; Ma, Jian Feng; Suga, Michihiro
  作者单位：Okayama University; Okayama University; Okayama University; Okayama University; Nanjing Agricultural University
  摘要：HvAACT1 is a major aluminum (Al)-tolerance gene in barley, encoding a citrate transporter that belongs to the multidrug and toxic compound extrusion (MATE) family. This transporter facilitates citrate secretion from the roots, thereby detoxifying external Al ions-a major constraint of crop production on acidic soils. In this study, we present the outward-facing crystal structure of HvAACT1, providing insights into a citrate transport mechanism. The putative citrate binding site consists of thr...
• Thermal mismatch models derived from occurrence data predict pathogen prevalence in frogs AI翻译
  作者：Duncan, Richard P.; Scheele, Ben C.; Clulow, Simon
  作者单位：University of Canberra; Australian National University
  摘要：Emerging infectious diseases increasingly threaten many wildlife populations, yet the impacts of pathogens vary considerably both within and among host species. The environmental tolerance mismatch hypothesis (ETMH) suggests that this variability stems in part from differences in the relative performance of hosts and pathogens under varying environmental conditions. According to the ETMH, pathogen impacts should be more severe in environments where pathogen performance is high and host perform...
• Cell type-specific purifying selection of synonymous mitochondrial DNA variation AI翻译
  作者：Lareau, Caleb A.; Maschmeyer, Patrick; Yin, Yajie; Gutierrez, Jacob C.; Dhindsa, Ryan S.; Burrer, Anne - Sophie Gribling -; Zielinski, Sebastian; Hsieh, Yu - Hsin; Nitsch, Lena; Dimitrova, Veronika; Nalbant, Benan; Buquicchio, Frank A.; Abay, Tsion; Stickels, Robert R.; Ulirsch, Jacob C.; Yan, Patrick; Wang, Fangyi; Miao, Zhuang; Sandor, Katalin; Daniel, Bence; Liu, Vincent; Mendez, Paul L.; Knaus, Petra; Meyer, Manpreet; Greenleaf, William J.; Kundaje, Anshul; Smyth, Redmond P.; Munschauer, Mathias; Ludwig, Leif S.; Satpathy, Ansuman T.
  作者单位：Memorial Sloan Kettering Cancer Center; Stanford University; Humboldt University of Berlin; Free University of Berlin; Charite Universitatsmedizin Berlin; Berlin Institute of Health; Helmholtz Association; Max Delbruck Center for Molecular Medicine; Baylor College of Medicine; Baylor College of Medicine; Baylor College Medical Hospital; Baylor College of Medicine; Helmholtz Association; Helmholtz-Center for Infection Research; Free University of Berlin; Harvard University; Harvard Medical School; Stanford University; Harvard University; Harvard University Medical Affiliates; Boston Children's Hospital; Harvard University; Harvard Medical School; Stanford University; Ruprecht Karls University Heidelberg
  摘要：While somatic variants are well-characterized drivers of tumor evolution, their influence on cellular fitness in nonmalignant contexts remains understudied. We identified a mosaic synonymous variant (m.7076A > G) in the mitochondrial DNA (mtDNA)-encoded cytochrome c-oxidase subunit 1 (MT-CO1, p.Gly391=), present at homoplasmy in 47% of immune cells from a healthy donor. Single-cell multiomics revealed strong, lineage-specific selection against the m.7076G allele in CD8+ effector memory T cells...
• Human land use promotes range expansion of soil protists from temperate to subtropical regions in China AI翻译
  作者：Li, Zhi-Peng; Sun, Xin; Yao, Haifeng; Shangguan, Hua-Yuan; Hu, Hang-Wei; Tang, Zhiyao; Li, Gang; Zhang, Weixin; Delgado-Baquerizo, Manuel; Scheu, Stefan; Zhu, Yong-Guan
  作者单位：Chinese Academy of Sciences; Institute of Urban Environment, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; University of Melbourne; Peking University; Peking University; Henan University; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto de Recursos Naturales y Agrobiologia de Sevilla (IRNAS); University of Gottingen; University of Gottingen
  摘要：Land- use changes are reshaping the distribution of aboveground species worldwide. However, the impact of land- use changes on the distribution of soil organisms remains poorly understood. In particular, we lack a mechanistic understanding of the environmental factors reshaping the distribution of soil microbiota in response to global biological homogenization. Here, we used metabarcoding to investigate the biogeography of protists and their relationships with prey and hosts in three human- do...
• Inter- and transdisciplinary knowledge is critical for nature- based solutions to contribute to just urban transformations AI翻译
  作者：Frantzeskaki, Niki; Wijsman, Katinka; Kabisch, Nadja; McPhearson, Timon
  作者单位：Utrecht University; Leibniz University Hannover; The New School; Cary Institute of Ecosystem Studies; Stockholm University; Royal Swedish Academy of Sciences; Beijer Institute of Ecological Economics
  摘要：Nature- based solutions (NBS) are used to transform existing unsustainable and undesirable path dependencies in cities. For NBS to contribute to just urban transformations, a stronger inter- and transdisciplinary knowledge base is needed. This knowledge base is essential to engage with six complex yet crucial questions about NBS, including for what?, which nature?, where?, how?, when, and for whom?. To address these questions, we identify two critical opportunities to advance the knowledge of ...
• De novo design of a fusion protein tool for GPCR research AI翻译
  作者：Gao, Kaixuan; Zhang, Xin; Nie, Jia; Meng, Hengyu; Zhang, Weishe; Tian, Boxue; Liu, Xiangyu
  作者单位：Tsinghua University; Tsinghua University; Central South University
  摘要：G protein-coupled receptors (GPCRs) play pivotal roles in cellular signaling and represent prominent drug targets. Structural elucidation of GPCRs is crucial for drug discovery efforts. However, structural studies of GPCRs remain challenging, particularly for inactive-state structures, which often require extensive protein engineering. Here, we present a de novo design strategy termed click fusion for generating fusion proteins to facilitate GPCR structural studies. Our method involves the rat...
• Paleobiome dynamics shaped a large Gondwanan plant radiation AI翻译
  作者：Skeels, Alexander; Sauquet, Herve; Mast, Austin; Weston, Peter H.; Olde, Peter M.; Jordan, Gregory J.; Carpenter, Raymond J.; Fenker, Jessica; Reynolds, Zoe K. M.; Lemmon, Alan R.; Lemmon, Emily Moriarty; Pichardo-Marcano, Fritz J.; Cardillo, Marcel
  作者单位：Australian National University; University of New South Wales Sydney; State University System of Florida; Florida State University; University of Tasmania; University of Adelaide; State University System of Florida; Florida State University
  摘要：Theories of adaptive radiation propose predictable trajectories in which diversity accumulates rapidly in newly formed or colonized environments with underexploited niche space and few competing species, before slowing down as competition intensifies, and speciation and extinction rates approach equilibrium. This historical perspective on diversity may be more important than current environmental variation for explaining today's biodiversity, but this has been difficult to determine because of...
• Ancient forests help document the long-term impacts of climate change AI翻译
  作者：Anderson, David G.
  作者单位：University of Tennessee System; University of Tennessee Knoxville
• Decoding and engineering temperature-sensitive lethality in Ceratitis capitata for pest control AI翻译
  作者：Aumann, Roswitha A.; Gouvi, Georgia; Gregoriou, Maria - Eleni; Rehling, Tanja; Sollazzo, Germano; Bourtzis, Kostas; Schetelig, Marc F.
  作者单位：Imperial College London; Justus Liebig University Giessen; Justus Liebig University Giessen; International Atomic Energy Agency; University of Patras
  摘要：The Sterile Insect Technique (SIT) is a species-specific and environmentally friendly method for effectively controlling pest insect populations based on releasing reared, sterile insects into infested areas. Sex sorting in rearing facilities, enabling male-only releases, is necessary to ensure SIT programs are efficient, cost-effective and, in case of mosquito control, also safe. This can be greatly facilitated by genetic sexing strains (GSS), exhibiting sex-specific phenotypic markers. Howev...