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作者：Lim, Donghoon; Park, Sewon; Lee, Jong Seung; Gwon, Hyo Jin; Nam, Yunwoo; Choi, Suhyuk; Kim, Jongwon; Kim, Yeonsu; Park, Geonwoo; Pyun, Woo Yang; Park, Hyun Woo; Cho, Seung- Woo; Ahn, Hyun S.
作者单位：Yonsei University; Yonsei University; Yonsei University; Institute for Basic Science - Korea (IBS); Yonsei University
摘要：Neural stem cells (NSCs) are valuable in the quest to conquer neurodegenerative diseases due to their capability to reconstruct the damaged neuronal networks. However, deep understanding of the intercellular signaling mechanism controlling the lineage and fate of the stem cells is required before potential clinical applications. Here, we applied nondestructive and label- free electrochemical methods for the longitudinal tracking of NSC respiratory metabolism. Sharp change in the oxygen utiliza...
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作者：Coste, Christophe F. D.
作者单位：Swansea University; Norwegian Institute Nature Research; Norwegian University of Science & Technology (NTNU); Universite Paris Cite; Centre National de la Recherche Scientifique (CNRS); Museum National d'Histoire Naturelle (MNHN); CNRS - Institute of Ecology & Environment (INEE)
摘要：Structured population projection models are fundamental to many fields of science. They enable abundance forecasting for populations categorized by various traits such as age (for demography), patch (for spatial ecology), genotype (for genetics), infectious stage (for epidemiology), or capital (economics). The demography of a structured population, determined by the transition rates (e.g., survival, fertility) between its various classes, also shapes its relatedness-or kinship-structure. This ...
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作者：Li, Chenwen; Zhao, Guanli; Qi, Yuantong; Sun, Menglong; Zhong, Ling; Yang, Qinghua; Hu, Kaiyao; Cheng, Juan; Dou, Yin; Hao, Yingxue; Zhang, Jianxiang
作者单位：Army Medical University; Army Medical University; Army Medical University
摘要：Cardiovascular diseases (CVDs), the primary cause of global mortality, face persistent therapeutic challenges due to poor drug targeting and systemic side effects. While innovative nanotherapeutic approaches offer improved drug delivery through targeting and stimulus-responsive release mechanisms, clinical translation is limited by poor tissue specificity and manufacturing limitations. We present a universal therapeutic hypothermia (TH, i.e., 32 to 35 degrees C)-enhanced strategy that amplifie...
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作者：Wang, Jinan; Nguyen, Anh T. N.; Adediwura, Victor A.; Lu, Cam Sinh; McNeill, Samantha M.; Jorg, Manuela; Scammells, Peter J.; Christopoulos, Arthur; May, Lauren T.; Miao, Yinglong
作者单位：University of North Carolina; University of North Carolina Chapel Hill; Monash University; Monash University; Monash University; Monash University
摘要：G protein-coupled receptors (GPCRs), the largest superfamily of human membrane proteins with >800 members, are primary targets for similar to 1/3 of all marketed drugs. Recent fluorescence experiments underscored the pivotal role of GPCR-G protein complex lifetime in their coupling efficiency and selectivity. However, these experiments are often expensive, time-consuming, and limited to a small number of GPCR-G protein systems. On the other hand, it is challenging to simulate GPCR-G protein di...
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作者：Zhang, Ge; Hu, Guoliang; Li, Jinlei; Feng, Guangxia; Cui, Yi; Chen, Zhouyi; Liu, Zaichun
作者单位：Stanford University; United States Department of Energy (DOE); Stanford University; SLAC National Accelerator Laboratory; Stanford University
摘要：Membrane-based technologies are essential for realizing sustainable ion-sieving processes such as lithium extraction. Much effort was devoted to designing new membrane materials, whereas the effect of charge distribution has been largely overlooked. Here, we filled this knowledge gap by providing a quantitative transport model for selective ion diffusion through a charge mosaic membrane (CMM). Composed of alternating regions of Li-selective ceramic and anion-selective polymeric materials, CMMs...
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作者：Avaro, Alexandre S.; Mirjalili, Shahab; Griffiths, Andrew D.; Santiago, Juan G.
作者单位：Stanford University; Universite PSL; Centre National de la Recherche Scientifique (CNRS); Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI); CNRS - Institute of Chemistry (INC); Royal Institute of Technology
摘要：The development of microfluidic technologies has enabled chemical and biological analysis systems with increased functionality, complexity, and parallelization. These functionalities often drive the creation and control of complex and dynamic fluidic architectures. Introduced here is a class of microfluidic network based on isotachophoresis (ITP), an electrokinetic process that can extract and purify samples, selectively transport, mix, and aliquot (split) samples in a system with no moving pa...
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作者：Bandini, Madison; Ghone, Dhaval; Evans III, Edward L.; Suzuki, Aussie; Sherer, Nathan M.; Smith, Janet
作者单位：University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison
摘要：The HIV type 1 (HIV-1) accessory proteins Virion Infectivity Factor (Vif) and Viral Protein R (Vpr) are essential for efficient virus replication in vivo. Vif mediates degradation of host restriction factor APOBEC3G, while Vpr modulates the host cell proteome in ways that promote virion infectivity and viral gene expression. In cycling cells including CD4+ T cells, Vif and Vpr also severely impact cell cycle progression for reasons that remain unknown. Here, we combined live-cell imaging with ...
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作者：Gilman, Morgan S. A.; Shlosman, Irina; Guerra, Daniel D. Same; Domecillo, Masy; Fivenson, Elayne M.; Bourett, Claire; Bernhardt, Thomas G.; Polizzi, Nicholas F.; Loparo, Joseph J.; Kruse, Andrew C.
作者单位：Harvard University; Harvard Medical School; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Harvard University; Harvard Medical School; Howard Hughes Medical Institute
摘要：The peptidoglycan (PG) cell wall is critical for bacterial growth and survival and is a primary antibiotic target. MreD is an essential accessory factor of the Rod complex, which carries out PG synthesis during elongation, yet little is known about how MreD facilitates this process. Here, we present the cryoelectron microscopy structure of Thermus thermophilus MreD in complex with another essential Rod complex component, MreC. The structure reveals that a periplasmic-facing pocket of MreD inte...
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作者：Jin, Ying; Egami, Naoki; Rothenhausler, Dominik
作者单位：Harvard University; Harvard University; Harvard Medical School; Columbia University; Stanford University; University of Pennsylvania; Massachusetts Institute of Technology (MIT)
摘要：Many existing approaches to generalizing statistical inference amid distribution shift operate under the covariate shift assumption, which posits that the conditional distribution of unobserved variables given observable ones is invariant across populations. However, recent empirical investigations have demonstrated that adjusting for shifts in observed variables (covariate shift) is often insufficient for generalization. In other words, covariate shift does not typically explain away the dist...
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作者：Stillman, Paul E.; Wilson, James D.; Kalkstein, David A.; Ferguson, Melissa J.
作者单位：Boston University; University of San Francisco; Yale University
摘要：Self-control-the ability to pursue long-term goals over short-term temptations-is a critical faculty of human cognition, but the cognitive processes enabling self-control are not well understood. Traditional models have focused on impulse inhibition: effort-fully inhibiting prepotent motor responses toward a temptation, yielding a stage-based evolution of choice. Other models emphasize dynamic competition between goal and temptation, yielding a more integrative evolution of choice. Although th...
