Longitudinal transformation of mitochondrial metabolism during neurogenesis

• 成果类型: Article
• 署名作者: Lim, Donghoon; Park, Sewon; Lee, Jong Seung; Gwon, Hyo Jin; Nam, Yunwoo; Choi, Suhyuk; Kim, Jongwon; Kim, Yeonsu; Park, Geonwoo; Pyun, Woo Yang; Park, Hyun Woo; Cho, Seung- Woo; Ahn, Hyun S.
• 署名单位: Yonsei University; Yonsei University; Yonsei University; Institute for Basic Science - Korea (IBS); Yonsei University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2517961122
• 发表日期: 2025-11-25
• 页码: e2517961122
• 关键词: neurogenesis; SCANNING ELECTROCHEMICAL MICROSCOPY; reactive oxygen species; intercellular signaling; neural stem-cells; reactive oxygen; hydrogen-peroxide; self-renewal; proliferation; differentiation; AQUAPORINS

摘要

Neural stem cells (NSCs) are valuable in the quest to conquer neurodegenerative diseases due to their capability to reconstruct the damaged neuronal networks. However, deep understanding of the intercellular signaling mechanism controlling the lineage and fate of the stem cells is required before potential clinical applications. Here, we applied nondestructive and label- free electrochemical methods for the longitudinal tracking of NSC respiratory metabolism. Sharp change in the oxygen utilization pattern was observed concomitant to stemness loss and onset of differentiation, suggesting metabolic reprogramming in the transition. Intra- and extracellular profiling of mitochondrial metabolites revealed molecular preference in the extracellular transport rates. Electrochemical emulation of the metabolite release pattern induced acceleration of neurite growth in nearby cells, suggesting paracrine signaling system mediated by mitochondrial metabolites.