-
作者：Watanabe, Kikyo; Oda-Yamamizo, Chihiro; Ishibashi, Kazuhiro
作者单位：National Agriculture & Food Research Organization - Japan
摘要：The genomic RNA of negative-strand RNA viruses is encapsidated by nucleocapsid proteins and associates with RNA polymerase to form a ribonucleoprotein (RNP) complex. Lacking both a 5' cap and a 3' poly (A) tail, viral RNAs are highly unstable and prone to degradation by cellular nucleases. Therefore, newly synthesized genomic and complementary-strand RNAs must be rapidly protected through RNP formation. However, the molecular mechanisms governing RNP assembly in cytoplasm-replicating negative-...
-
作者：Leithner, Alexander; Kvalvaag, Audun; Mitra, Tanmay; Valvo, Salvatore; Dada, Hannah; Compeer, Ewoud; Barton, Michael I.; Eguiguren, Sofia Bustamante; Jenkins, Edward; Lagerholm, Christoffer; Dushek, Omer; Dustin, Michael L.
作者单位：University of Oxford; Kennedy Institute for Rheumatology; University of Oslo; National Hospital Norway; Chinese Academy of Medical Sciences - Peking Union Medical College; University of Oxford; University of Oxford; Salzburg University
摘要：The immunological synapse (IS) integrates antigen recognition and adhesion to control T cell activation and effector functions. Reductionist systems have been instrumental dissecting IS organization, but conventional systems constrain all ligands to be either mobile or immobile, unlike antigen-presenting cells where intercellular adhesion molecule 1 (ICAM1) is cytoskeletally anchored while T cell receptor (TCR) ligands remain mobile. Here, we establish mixed-mobility supported lipid bilayers (...
-
作者：Maisonet, Isabella Jaen; Sharafi, Mona; Korchak, Emilie J.; Salazar-Chaparro, Andres; Bratt, Ariana S.; Parikh, Tvesha; Varca, Anthony C.; Shah, Binita; Darnowski, Michael; Chung, Mia; Teh, Wei Pin; Che, Jianwei; Bezsonova, Irina; Buhrlage, Sara J.
-
作者：Agrawal, Vipin; de la Cruz, Monica Olvera
作者单位：Northwestern University; Northwestern University
摘要：Multicomponent crystalline and amorphous elastic shells exhibit heterogeneous surface patterns that provide distinctive functionalities in cellular environments. Such patterning typically arises from the competition between short-range attractive and long-range repulsive interactions in membranes. Here, we demonstrate that the intrinsic competition between electrostatic repulsion and elastic deformation is sufficient to drive spontaneous surface patterning in elastic shells, requiring no addit...
-
作者：Bull, Tawni; Van Blair, Jared; Sutton, Sam; Colwell, Hadley; Khakhar, Arjun
作者单位：Colorado State University System; Colorado State University Fort Collins
摘要：Enhancements to crop morphology, such as the semidwarfing that helped drive the green revolution, are often driven by changes in gene expression. These are challenging to translate across species, which slows the rate of crop improvement. Synthetic transcription factors (SynTFs) offer a rapid alternative to generate targeted alterations to gene expression. However, the complexity of developmental pathways makes it unclear how to best apply them to predictably engineer morphology. In this work,...
-
作者：Guo, Haoyu; Tikhanovskaya, Maria; Raccuglia, Paul; Vlaskin, Alexey; Co, Chris; Liebling, Daniel J.; Ellsworth, Scott; Abraham, Matthew; Dorfman, Elizabeth; Armitage, N. P.; Feng, Chunhan; Georges, Antoine; Gingras, Olivier; Kiese, Dominik; Kivelson, Steven A.; Oganesyan, Vadim; Ramshaw, B. J.; Sachdev, Subir; Senthil, T.; Tranquada, J. M.; Brenner, Michael P.; Venugopalan, Subhashini; Kim, Eun-Ah
作者单位：Cornell University; Alphabet Inc.; Google Incorporated; Harvard University; Johns Hopkins University; Simons Foundation; Flatiron Institute; Universite PSL; College de France; Centre National de la Recherche Scientifique (CNRS); Institut Polytechnique de Paris; Ecole Polytechnique; University of Geneva; CEA; Centre National de la Recherche Scientifique (CNRS); Universite Paris Saclay; Stanford University; City University of New York (CUNY) System; City University of New York (CUNY) System; College of Staten Island (CUNY); Massachusetts Institute of Technology (MIT); United States Department of Energy (DOE); Brookhaven National Laboratory; Harvard University; Ewha Womans University
摘要：Large Language Models (LLMs) show great promise as a powerful tool for scientific literature exploration. However, their effectiveness in providing scientifically accurate and comprehensive answers to complex questions within specialized domains remains an active area of research. Using the field of high-temperature cuprates as an exemplar, we evaluate the ability of LLM systems to understand the literature at the level of an expert. We construct an expert-curated database of 1,726 scientific ...
-
作者：Aronson, Heidi S.; Weaver Jr, Matt E.; Hu, Ruiwen; Lanclos, V. Celeste; Rapp, Jacob D.; Iavarone, Anthony T.; Carlson, Hans K.; Coates, John D.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of California System; University of California Berkeley
摘要：The anaerobic conversion of organic matter to methane and carbon dioxide typically relies on obligate syntrophic interactions between bacteria and methanogenic archaea, where interspecies electron transfer enables thermodynamically constrained reactions to proceed near equilibrium. Syntrophs often couple the oxidation of fermentation products such as fatty acids and alcohols to the reduction of protons to form hydrogen (H2). These reactions can only proceed if low H2 concentrations are maintai...
-
作者：Hu, Qiaoyu; Cao, Yu; Ren, PengXuan; Zhang, Xianglei; Li, Fenglei; Zhang, Xueyuan; Cai, Fengyu; Zhang, Ran; Zhou, Yongqi; Mei, Lianghe; Bai, Fang
作者单位：ShanghaiTech University; ShanghaiTech University; Shanxi Medical University; ShanghaiTech University; Chinese Academy of Sciences
摘要：Targeted protein degradation is a promising strategy for drug discovery, but designing effective PROTACs remains challenging, especially for proteins without well-defined binding sites. Current methods rely on modifying linkers between fixed ligands, which limits the diversity and innovation of the overall molecular architecture of PROTAC. Here, we introduce DeepDegradome, an AI-powered method that automates the structure-aware design of both small-molecule ligands and PROTACs. It employs a la...
-
作者：Ramos - Almodovar, Fabian; Gao, Ziyue; Voight, Benjamin F.; Mathieson, Iain
作者单位：University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine
摘要：Mutation spectra vary across genetic and environmental contexts, leading to differences between and within species. Most research on mutation spectrum has focused on trinucleotide (3-mer) mutation types in mammals, limiting the breadth and depth of variation surveyed. In this study, we use whole-genome resequencing data across 108 eukaryotic species-including mammals, fish, plants, and invertebrates-to characterize pentanucleotide (5-mer) noncoding mutation spectra using a Bayesian approach. O...
-
作者：Chen, Xin
作者单位：Howard Hughes Medical Institute; Johns Hopkins University
