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作者：Skopintsev, Petr; Esain-Garcia, Isabel; DeTurk, Evan C.; Yoon, Peter H.; Zhou, Zehan; Weiss, Trevor; Kamalu, Maris; Chamraj, Ajit; Loi, Kenneth J.; Langeberg, Conner J.; Boger, Ron S.; Nisonoff, Hunter; Karp, Hannah M.; Chen, Lin-Xing; Shi, Honglue; Vohra, Kamakshi; Banfield, Jillian F.; Cate, Jamie H. D.; Jacobsen, Steven E.; Doudna, Jennifer A.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Los Angeles; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; Monash University; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of California System; University of California Los Angeles; Howard Hughes Medical Institute; University of California System; University of California Berkeley; University of California System; University of California Berkeley
摘要：The design of RNA-guided nucleases with properties not limited by evolution can expand programmable genome-editing capabilities. However, generating diverse multidomain proteins with robust enzymatic properties remains challenging. Here, we use a protein design strategy that couples a structure-guided inverse-folding model with evolution-informed residue constraints to generate active, divergent variants of TnpB, a minimal CRISPR-Cas12-like nuclease, termed SynTnpBs. High-throughput screening ...
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作者：Ruhlemann, Carsten
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作者：Tan, Yuting Jessy; Conley, Travis E.; Yao, Fuwen; Garcia-Marques, Fernando J.; Akinyemi, Damilola E.; Dinh, Van Vuong; Wang, Qian; Bermudez, Abel; Kim, Jieun; Belk, Julia A.; Soehnlein, Oliver; Pitteri, Sharon J.; Andreasson, Katrin I.
作者单位：Stanford Medicine; Stanford University; Stanford Medicine; Stanford University; Stanford University; Stanford Medicine; Stanford University; Stanford University; Stanford University; Chan Zuckerberg Initiative (CZI)
摘要：Aging disrupts tissue homeostasis across organ systems. Here, we identify tissue-resident macrophages (TRMs) as central coordinators of age-related organ decline through impaired clearance of senescent neutrophils, a process regulated by the immunomodulatory prostaglandin E2 (PGE2) receptor EP2. Reducing TRM EP2 signaling in aged mice preserved youthful mitochondrial fitness and prevented cognitive decline, frailty, sarcopenia, adiposity, cardiac impairment, and systemic inflammation. Plasma p...
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作者：Cui, Wenjun; Qian, Cheng; Lin, Weixiao; Xue, Zefan; Ge, Zhencui; Zhao, Wen; Van Tendeloo, Gustaaf; Wu, Jinsong; Ding, Feng; Sang, Xiahan; Fu, Zhengyi
作者单位：Wuhan University of Technology; Wuhan University of Technology; Wuhan University of Technology; Anhui University; Suzhou Laboratory; China University of Petroleum; University of Antwerp
摘要：Atomic-resolution observation of the liquid-solid phase transition within a geometrically confined nanocluster provides fundamental insights into heterogeneous nucleation mechanisms. In this work, using in situ transmission electron microscopy, we directly control and observe a single critical-sized bismuth nanocluster within a tunable nanoscale gap, driving it through a reversible cycle from quasi-amorphous nanodisc, to crystalline nanowire, to liquid nanodroplet. The cluster's aspect ratio, ...
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作者：FitzGerald, Garret A.
作者单位：University of Pennsylvania; Pennsylvania Medicine
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作者：Cornwall, Warren
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作者：Meng, Shaoshuai
作者单位：Peking University
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作者：Cheon, Yeryun; Kiani, Mehrdad T.; Tu, Yi-Hsin; Kumar, Sushant; Duong, Nghiep Khoan; Kim, Jiyoung; Kong, Lingcheng; Sam, Quynh P.; Wang, Han; Kushwaha, Satya K.; Ng, Nicholas; Lee, Seng Huat; Kielar, Sam; Li, Chen; Schaeffer, Amelia; Coyle, Jack D.; Koumoulis, Dimitrios; Siddique, Saif; Mao, Zhiqiang; Jin, Gangtae; Tian, Zhiting; Sundararaman, Ravishankar; Lin, Hsin; Liang, Gengchiau; Chen, Ching-Tzu; Cha, Judy J.
作者单位：Cornell University; Cornell University; National Yang Ming Chiao Tung University; International Business Machines (IBM); IBM USA; Cornell University; Johns Hopkins University; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Cornell University; Cornell University; Gachon University; Rensselaer Polytechnic Institute; Academia Sinica - Taiwan; International Business Machines (IBM); IBM USA
摘要：Ongoing demands for smaller and more energy-efficient electronic devices necessitate alternative interconnect materials with lower electrical resistivity at reduced dimensions. We report the synthesis of Weyl semimetal niobium arsenide (NbAs) nanowires through thermomechanical nanomolding with single crystallinity and controlled diameters down to 40 nanometers. The resistivity of NbAs nanowires decreases with decreasing diameter, and 40-nanometer-diameter nanowires exhibited a room-temperature...
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作者：Conn, Chandler J.; Yu, Phelan; Howard, Madison I.; Yang, Yuxi; Zhang, Chaoqun; Jadbabaie, Arian; Gorou, Aikaterini; Gaiser, Alyssa N.; Steimle, Timothy C.; Cheng, Lan; Hutzler, Nicholas R.
作者单位：California Institute of Technology; Johns Hopkins University; Michigan State University; Michigan State University
摘要：Molecules with heavy, radioactive nuclei promise extreme sensitivity to fundamental nuclear and particle physics. However, these nuclei are available in limited quantities, which challenges their use in precision measurements. Here we demonstrate the gas-phase synthesis, cryogenic cooling, and high-resolution laser spectroscopy of radium monohydroxide, monodeuteroxide, and monofluoride molecules (226RaOH, 226RaOD, and 226RaF) in a tabletop apparatus by combining trace radioactive target produc...
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作者：Stone, Richard
