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作者：Magkiriadou, Sofia; Stepp, Willi L.; Newman, Dianne K.; Manley, Suliana; Racki, Lisa R.
作者单位：Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; California Institute of Technology; California Institute of Technology; Scripps Research Institute
摘要：Polyphosphate (polyP) synthesis is a ubiquitous stress and starvation response in bacteria. In diverse species, mutants unable to make polyP have a wide variety of physiological defects, but the mechanisms by which this simple polyanion exerts its effects remain unclear. One possibility is that polyP's many functions stem from global effects on the biophysical properties of the cell. We characterize the effect of polyphosphate on cytoplasmic mobility under nitrogen-starvation conditions in the...
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作者：Tang, Hesen; Liu, Zhengjie; Han, Guangmei; Geng, Junlong; Liu, Bianhua; Zhang, Ruilong; Zhang, Zhongping
作者单位：Anhui University; Chinese Academy of Sciences; Hefei Institutes of Physical Science, CAS
摘要：Omega - 3 polyunsaturated fatty acids (PUFA) found primarily in fish oil have been a popular supplement for cardiovascular health because they can substantially reduce circulating triglyceride levels in the bloodstream to prevent atherosclerosis. Beyond this established extracellular activity, here, we report a mode of action of PUFA, regulating intracellular triglyceride metabolism and lipid droplet (LD) dynamics. Real - time imaging of the subtle and highly dynamic changes of intracellular l...
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作者：Bach, Eviatar; Krishnamurthy, V.; Mote, Safa; Shukla, Jagadish; Sharma, A. Surjalal; Kalnay, Eugenia; Ghil, Michael
作者单位：California Institute of Technology; California Institute of Technology; Center for Ocean Land Atmosphere Studies; George Mason University; Portland State University; University System of Maryland; University of Maryland College Park; George Mason University; University System of Maryland; University of Maryland College Park; Universite PSL; Ecole Normale Superieure (ENS); Universite PSL; Ecole Normale Superieure (ENS); Institut Polytechnique de Paris; Ecole Polytechnique; Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Earth Sciences & Astronomy (INSU); Universite PSL; Ecole Normale Superieure (ENS); Universite Paris Saclay; Universite PSL; University of California System; University of California Los Angeles; Imperial College London
摘要：Predicting the temporal , spatial patterns of South Asian monsoon rainfall within a season is of critical importance due to its impact on agriculture, water availability , flooding. The monsoon intraseasonal oscillation (MISO) is a robust northward - propagating mode that determines the active and break phases of the monsoon and much of the regional distribution of rainfall. However, dynamical atmospheric forecast models predict this mode poorly. Data -driven methods for MISO prediction have s...
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作者：Deng, Hu; Liu, Lingyu; Tang, Xiaping; Lu, Yingxian; Wang, Xiaofei; Zhao, Yanyu; Shi, Yigong
作者单位：Tsinghua University; Tsinghua University; Peking University; Westlake Laboratory; Westlake University; Westlake University; Westlake University
摘要：Electromagnetic radiation (EMR) in the environment, particularly in the microwave range, may constitute a public health concern. Exposure to 2.4 GHz EMR modulated by 100 Hz square pulses was recently reported to markedly increase wakefulness in mice. Here, we demonstrate that a similar wakefulness increase can be induced by the modulation frequency of 1,000 Hz, but not 10 Hz. In contrast to the carrier frequency of 2.4 GHz, 935 MHz EMR of the same power density has little impact on wakefulness...
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作者：Wen, Xiaohong; Huang, Zhixiang; Yang, Xiaohai; He, Xiaoxiao; Li, Lie; Chen, Haiyan; Wang, Kemin; Guo, Qiuping; Liu, Jianbo
作者单位：Hunan University; Hunan University; Hunan University; Hunan University
摘要：Multidrug resistance (MDR) is a major factor in the failure of many forms of tumor chemotherapy. Development of a specific ligand for MDR- reversal would enhance the intracellular accumulation of therapeutic agents and effectively improve the tumor treatments. Here, an aptamer was screened against a doxorubicin (DOX)- resistant human hepatocellular carcinoma cell line (HepG2/DOX) via cell - based systematic evolution of ligands by exponential enrichment. A 50 nt truncated sequence termed d3 wa...
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作者：Frain, Kelly M.; Dedic, Emil; Nel, Lynette; Bohush, Anastasiia; Olesen, Esben; Thaysen, Katja; Wustner, Daniel; Stokes, David L.; Pedersen, Bjorn Panyella
作者单位：Aarhus University; Aarhus University; University of Southern Denmark; New York University
摘要：The membrane protein Niemann-Pick type C1 (NPC1, named NCR1 in yeast) is central to sterol homeostasis in eukaryotes. Saccharomyces cerevisiae NCR1 is localized to the vacuolar membrane, where it is suggested to carry sterols across the protective glycocalyx and deposit them into the vacuolar membrane. However, documentation of a vacuolar glycocalyx in fungi is lacking, and the mechanism for sterol translocation has remained unclear. Here, we provide evidence supporting the presence of a glyco...
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作者：Sun, Pengchuan; Lu, Zhiqiang; Wang, Zhenyue; Wang, Shang; Zhao, Kexin; Mei, Dong; Yang, Jiao; Yang, Yongzhi; Renner, Susanne S.; Liu, Jianquan
作者单位：Sichuan University; Chinese Academy of Sciences; Xishuangbanna Tropical Botanical Garden, CAS; Lanzhou University; Washington University (WUSTL)
摘要：Malvaceae comprise some 4,225 species in 243 genera and nine subfamilies and include economically important species, such as cacao, cotton, durian, and jute, with cotton an important model system for studying the domestication of polyploids. Here, we use chromosome - level genome assemblies from representatives of five or six subfamilies (depending on the placement of Ochroma) to differentiate coexisting subgenomes and their evolution during the family's deep history. The results reveal that t...
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作者：Yasuhara, Moriaki; Deutsch, Curtis A.; Zhang, Jingwen
作者单位：University of Hong Kong; University of Hong Kong; City University of Hong Kong; Princeton University; Princeton University
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作者：Biswas, Avik; Choudhuri, Indrani; Arnold, Eddy; Lyumkis, Dmitry; Haldane, Allan; Levy, Ronald M.
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); Temple University; Salk Institute; University of California System; University of California San Diego; Pennsylvania Commonwealth System of Higher Education (PCSHE); Temple University; Rutgers University System; Rutgers University New Brunswick; University of California System; University of California San Diego; Pennsylvania Commonwealth System of Higher Education (PCSHE); Temple University
摘要：Drug resistance in HIV type 1 (HIV - 1) is a pervasive problem that affects the lives of millions of people worldwide. Although records of drug- resistant mutations (DRMs) have been extensively tabulated within public repositories, our understanding of the evolutionary kinetics of DRMs and how they evolve together remains limited. Epistasis, the interaction between a DRM and other residues in HIV - 1 protein sequences, is key to the temporal evolution of drug resistance. We use a Potts sequenc...
