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作者：Vermeij, Geerat J.
作者单位：University of California System; University of California Davis
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作者：Koo, Jinmo; Palli, Subba Reddy
作者单位：University of Kentucky; University of California System; University of California Riverside
摘要：RNA interference (RNAi) is more efficient in coleopteran insects than other insects. StaufenC (StauC), a coleopteran - specific double - stranded RNA (dsRNA) - binding protein, is required for efficient RNAi in coleopterans. We investigated the function of StauC in the intracellular transport of dsRNA into the cytosol, where dsRNA is digested by Dicer enzymes and recruited by Argonauts to RNA - induced silencing complexes. Confocal microscopy and cellular organelle fractionation studies have s...
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作者：Lee, Soah; Roest, Alison S. Vander; Blair, Cheavar A.; Kao, Kerry; Bremner, Samantha B.; Childers, Matthew C.; Pathak, Divya; Heinrich, Paul; Lee, Daniel; Chirikian, Orlando; Mohran, Saffie E.; Roberts, Brock; Smith, Jacqueline E.; Jahng, James W.; Paik, David T.; Wu, Joseph C.; Gunawardane, Ruwanthi N.; Ruppel, Kathleen M.; Mack, David L.; Pruitt, Beth L.; Regnier, Michael; Wu, Sean M.; Spudich, James A.; Bernstein, Daniel
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作者：Zhang, Jia; Singh, Poonam; Engel, Dieter; Fingerhut, Benjamin P.; Broser, Matthias; Hegemann, Peter; Elsaesser, Thomas
作者单位：Leibniz Association; Max Born Institute for Nonlinear Optics & Short Term Spectroscopy; University of Munich; University of Munich; Humboldt University of Berlin
摘要：The photoinduced all -trans to 13 -cis isomerization of the retinal Schiff base represents the ultrafast first step in the reaction cycle of bacteriorhodopsin (BR). Extensive experimental and theoretical work has addressed excited -state dynamics and isomerization via a conical intersection with the ground state. In conflicting molecular pictures, the excited state potential energy surface has been modeled as a pure S 1 state that intersects with the ground state, or in a 3 -state picture invo...
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作者：Guo, Jiashu; Zhang, Peipei; Wu, Nan; Liu, Wenwen; Liu, Yan; Jin, Huaibing; Francis, Frederic; Wang, Xifeng
作者单位：Chinese Academy of Agricultural Sciences; Institute of Plant Protection, CAAS; University of Liege; Langfang Normal University; Chinese Academy of Agricultural Sciences
摘要：Although most known viruses infecting fungi pathogenic to higher eukaryotes are asymptomatic or reduce the virulence of their host fungi, those that confer hypervirulence to entomopathogenic fungus still need to be explored. Here, we identified and studied a novel mycovirus in Metarhizium flavoviride, isolated from small brown planthopper (Laodelphax striatellus). Based on molecular analysis, we tentatively designated the mycovirus as Metarhizium flavoviride partitivirus 1 (MfPV1), a species i...
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作者：Nam, Chehyun; Li, Li-Yan; Yang, Qian; Ziman, Benjamin; Zhao, Hua; Hu, Boyan; Collet, Casey; Jing, Pei; Lei, Qifang; Xu, Li-Yan; Li, En- Min; Koeffler, H. Phillip; Sinha, Uttam K.; Lin, De- Chen
作者单位：University of Southern California; Shantou University; Cedars Sinai Medical Center; Shenzhen University; University of Southern California
摘要：Upper aerodigestive squamous cell carcinoma (UASCC) is a common and aggressive malignancy with few effective therapeutic options. Here, we investigate amino acid metabolism in this cancer, surprisingly noting that UASCC exhibits the highest methionine level across all human cancers, driven by its transporter LAT1. We show that LAT1 is also expressed at the highest level in UASCC, transcriptionally activated by UASCC - specific promoter and enhancers, which are directly coregulated by SCC maste...
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作者：Homma, Masato; Wakabayashi, Takatoshi; Moriwaki, Yoshitaka; Shiotani, Nanami; Shigeta, Takumi; Isobe, Kazuki; Okazawa, Atsushi; Ohta, Daisaku; Terada, Tohru; Shimizu, Kentaro; Mizutani, Masaharu; Takikawa, Hirosato; Sugimoto, Yukihiro
作者单位：Kobe University; University of Tokyo; University of Tokyo; University of Tokyo; Osaka Metropolitan University; Osaka Metropolitan University; Institute of Science Tokyo; Tokyo Medical & Dental University (TMDU)
摘要：Strigolactones (SLs) are plant apocarotenoids with diverse roles and structures. Canonical SLs, widespread and characterized by structural variations in their tricyclic lactone (ABC- ring), are classified into two types based on C- ring configurations. The steric C- ring configuration emerges during the BC- ring closure, downstream of the biosynthetic intermediate, carlactonoic acid (CLA). Most plants produce either type of canonical SLs stereoselectively, e.g., tomato (Solanum lycopersicum) y...
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作者：Sledzieski, Samuel; Kshirsagar, Meghana; Baek, Minkyung; Dodhia, Rahul; Ferres, Juan Lavista; Berger, Bonnie
作者单位：Microsoft; Massachusetts Institute of Technology (MIT); Seoul National University (SNU); Massachusetts Institute of Technology (MIT)
摘要：Proteomics has been revolutionized by large protein language models (PLMs), which learn unsupervised representations from large corpora of sequences. These models are typically fine-tuned in a supervised setting to adapt the model to specific downstream tasks. However, the computational and memory footprint of fine-tuning (FT) large PLMs presents a barrier for many research groups with limited computational resources. Natural language processing has seen a similar explosion in the size models,...
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作者：Hille, Bertil; Mackie, Ken; Lai, Yvonne Y.
作者单位：University of Washington; University of Washington Seattle; Indiana University System; Indiana University Bloomington; Indiana University System; Indiana University Bloomington
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作者：Kim, Seungjoon; Jang, Gyubin; Kim, Hyeonho; Lim, Dongseok; Han, Kyung Ah; Um, Ji Won; Ko, Jaewon
作者单位：Daegu Gyeongbuk Institute of Science & Technology (DGIST); Daegu Gyeongbuk Institute of Science & Technology (DGIST)
摘要：MDGA (MAM domain containing glycosylphosphatidylinositol anchor) family proteins were previously identified as synaptic suppressive factors. However, various genetic manipulations have yielded often irreconcilable results, precluding precise evaluation of MDGA functions. Here, we found that, in cultured hippocampal neurons, conditional deletion of MDGA1 and MDGA2 causes specific alterations in synapse numbers, basal synaptic transmission, and synaptic strength at GABAergic and glutamatergic sy...
