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作者：Auspurg, Katrin; Bruederl, Josef
作者单位：University of Munich
摘要：We discuss a relatively new meta- scientific research design: many- analyst studies that attempt to assess the replicability and credibility of research based on largescale observational data. In these studies, a large number of analysts try to answer the same research question using the same data. The key idea is the greater the variation in results, the greater the uncertainty in answering the research question and, accordingly, the lower the credibility of any individual research finding. C...
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作者：Li, Tong; Yum, Seoyun; Wu, Junjiao; Li, Minghao; Deng, Yafang; Sun, Lijun; Zuo, Xiaoxia; Chen, Zhijian J.
作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; Central South University; Central South University; Howard Hughes Medical Institute
摘要：Detection of cytosolic DNA by the cyclic GMP-AMP (cGAMP) synthase (cGAS)- stimulator of interferon genes (STING) pathway provides immune defense against pathogens and cancer but can also cause autoimmunity when overactivated. The exonuclease three prime repair exonuclease 1 (TREX1) degrades DNA in the cytosol and prevents cGAS activation by self- DNA. Loss- of- function mutations of the TREX1 gene are linked to autoimmune diseases such as Aicardi-Gouti & egrave;res syndrome, and mice deficient...
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作者：Schleske, Jonas M.; Hubrich, Jasmine; Wirth, Jan Otto; D'Este, Elisa; Engelhardt, Johann; Hell, Stefan W.
作者单位：Max Planck Society
摘要：Dynein is the primary molecular motor responsible for retrograde intracellular transport of a variety of cargoes, performing successive nanometer- sized steps within milliseconds. Due to the limited spatiotemporal precision of established methods for molecular tracking, current knowledge of dynein stepping is essentially limited to slowed- down measurements in vitro. Here, we use MINFLUX fluorophore localization to directly track CRISPR/Cas9- tagged endogenous dynein with nanometer/millisecond...
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作者：Baker, Rosanna P.; Liu, Amy Z.; Casadevall, Arturo
作者单位：Johns Hopkins University; Johns Hopkins Bloomberg School of Public Health
摘要：Cryptococcus neoformans has emerged as a frontrunner among deadly fungal pathogens and is particularly life- threatening for many HIV- infected individuals with compromised immunity. Multiple virulence factors contribute to the growth and survival of C. neoformans within the human host, the two most prominent of which are the polysaccharide capsule and melanin. As both of these features are associated with the cell wall, we were interested to explore possible cooperative or competitive interac...
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作者：Komarova, Natalia L.; Rignot, Chiara; Fleischman, Angela G.; Wodarz, Dominik
作者单位：University of California System; University of California San Diego; University of California System; University of California Irvine; University of California System; University of California Irvine; University of California System; University of California San Diego
摘要：A major next step in hematopoietic stem cell (HSC) biology is to enhance our quantitative understanding of cellular and evolutionary dynamics involved in undisturbed hematopoiesis. Mathematical models have been and continue to be key in this respect, and are most powerful when parameterized experimentally and containing sufficient biological complexity. In this paper, we use data from label propagation experiments in mice to parameterize a mathematical model of hematopoiesis that includes home...
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作者：Shuvaev, Sergey; Lachi, Divyansha; Koulakov, Alexei; Zador, Anthony
摘要：Animals are born with extensive innate behavioral capabilities, which arise from neural circuits encoded in the genome. However, the information capacity of the genome is orders of magnitude smaller than that needed to specify the connectivity of an arbitrary brain circuit, indicating that the rules encoding circuit formation must fit through a genomic bottleneck as they pass from one generation to the next. Here, we formulate the problem of innate behavioral capacity in the context of artific...
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作者：Sauciuc, Adina; Whittaker, Jacob; Tadema, Matthijs; Tych, Katarzyna; Guskov, Albert; Maglia, Giovanni
作者单位：University of Groningen
摘要：The transport of biopolymers across nanopores is an important biological process currently under investigation for the rapid analysis of DNA and proteins. While the transport of DNA is generally understood, methods to induce unfolded protein translocation that during electroosmotically driven translocation of polypeptides, blob- like structures typically form inside nanopores, often obstructing their transport and preventing addressing individual amino acids. This is in contrast with the elect...
