2024

最新文章

• Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont AI翻译
  作者：Motta, Erick V. S.; Lariviere, Patrick J.; Jones, Korin R.; Song, Yulin; Moran, Nancy A.
  作者单位：University of Texas System; University of Texas Austin; Texas A&M University System; Texas A&M University College Station; University of Texas System; University of Texas Austin
  摘要：The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram- negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, a core bacterial symbiont of the honey bee gut microbiota. We generated single- and double- knockout mutants targeting essential genes ( tssD and tssE) in both T6SS-1 and T6SS-2 and assessed their colon...
• Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin (vol 121, e2415544121, 2024) AI翻译
  作者：Garzon, Ramiro; Garofalo, Michela; Martelli, Maria Paola; Briesewitz, Roger; Wang, Lisheng; Fernandez-Cymering, Cecilia; Volinia, Stefano; Liu, Chang-Gong; Schnittger, Susanne; Haferlach, Torsten; Liso, Arcangelo; Diverio, Daniela; Mancini, Marco; Meloni, Giovanna; Foa, Robin; Martelli, Massimo F.; Mecucci, Cristina; Croce, Carlo M.; Falini, Brunangelo
• The neurotrophic factor MANF regulates autophagy and lysosome function to promote proteostasis in Caenorhabditis elegans AI翻译
  作者：Taylor, Shane K. B.; Hartman, Jessica H.; Gupta, Bhagwati P.
  作者单位：McMaster University; Medical University of South Carolina; Medical University of South Carolina
  摘要：The conserved mesencephalic astrocyte- derived neurotrophic factor (MANF) is known for protecting dopaminergic neurons and functioning in various other tissues. Previously, increased endoplasmic reticulum (ER) stress, dopaminergic neurodegeneration, and abnormal protein aggregation. These findings suggest an essential role for MANF in cellular processes. However, the mechanisms by which intracellular and extracellular MANF regulate broader cellular functions remain unclear. We report a unique ...
• Correlated topological flat bands in rhombohedral graphite AI翻译
  作者：Zhang, Hongyun; Li, Qian; Scheer, Michael G.; Wang, Renqi; Tuo, Chuyi; Zou, Nianlong; Chen, Wanying; Li, Jiaheng; Cai, Xuanxi; Bao, Changhua; Li, Ming-Rui; Deng, Ke; Watanabe, Kenji; Taniguchi, Takashi; Ye, Mao; Tang, Peizhe; Xu, Yong; Yu, Pu; Avila, Jose; Dudin, Pavel; Denlinger, Jonathan D.; Yao, Hong; Lian, Biao; Duan, Wenhui; Zhou, Shuyun
  作者单位：Tsinghua University; Tsinghua University; Princeton University; Tsinghua University; National Institute for Materials Science; National Institute for Materials Science; Chinese Academy of Sciences; Shanghai Advanced Research Institute, CAS; Beihang University; Max Planck Society; SOLEIL Synchrotron; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory
  摘要：Flat bands and nontrivial topological physics are two important topics of condensed matter physics. With a unique stacking configuration analogous to the Su-Schrieffer- Heeger model, rhombohedral graphite (RG) is a potential candidate for realizing both flat bands and nontrivial topological physics. Here, we report experimental evidence of topological flat bands (TFBs) on the surface of bulk RG, which are topologically protected by bulk helical Dirac nodal lines via the bulk-boundary correspon...
• Native β- barrel substrates pass through two shared intermediates during folding on the BAM complex AI翻译
  作者：dos Santos, Thiago M. A.; Thomson, Benjamin D.; Marquez, Melissa D.; Pan, Lydia; Monfared, Tabasom H.; Kahne, Daniel E.
  作者单位：Northeastern University; Harvard University
  摘要：The assembly of beta- barrel proteins into membranes is mediated by the evolutionarily numerous substrates which vary considerably in size and shape. How BAM is able to efficiently fold such a diverse array of beta- barrel substrates is not clear. Here, we develop a disulfide crosslinking method to trap native substrates in vivo as they fold on BAM. By placing a cysteine within the luminal wall of the BamA barrel as well as in the substrate beta- strands, we can compare the residence time of e...
• Identification of a cell- active chikungunya virus nsP2 protease inhibitor using a covalent fragment- based screening approach AI翻译
  作者：Merten, Eric M.; Sears, John D.; Leisner, Tina M.; Hardy, P. Brian; Ghoshal, Anirban; Hossain, Mohammad Anwar; Asressu, Kesatebrhan Haile; Brown, Peter J.; Tse, Edwin G.; Stashko, Michael A.; Li, Kelin; Drouin, Jacqueline L. Norris -; Herring, Laura E.; Mordant, Angie L.; Webb, Thomas S.; Mills, Christine A.; Barker, Natalie K.; Streblow, Zachary J.; Perveen, Sumera; Arrowsmith, Cheryl H.; Counago, Rafael Miguez; Arnold, Jamie J.; Cameron, Craig E.; Streblow, Daniel N.; Moorman, Nathaniel J.; Heise, Mark T.; Willson, Timothy M.; Popov, Konstantin I.; Pearce, Kenneth H.
  作者单位：University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; Oregon Health & Science University; University of Toronto; Structural Genomics Consortium; Universidade Estadual de Campinas; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine
  摘要：Chikungunya virus (CHIKV) is a mosquito- borne alphavirus that has been responsible for numerous large- scale outbreaks in the last twenty years. Currently, there are no FDA- approved therapeutics for any alphavirus infection. CHIKV nonstructural protein 2 (nsP2), which contains a cysteine protease domain, is essential for viral replication, making it an attractive target for a drug discovery campaign. Here, we optimized a CHIKV nsP2 protease (nsP2pro) biochemical assay for the screening of a ...
• TRIM21 induces selective autophagic degradation of c- Myc and sensitizes regorafenib therapy in colorectal cancer AI翻译
  作者：Ye, Wen-Long; Huang, Long; Yang, Xiao-Qin; Wan, Shan; Gan, Wen-Juan; Yang, Yun; He, Xiao-Shun; Liu, Feng; Guo, Xin; Liu, Yi-Xuan; Hu, Guang; Li, Xiu-Ming; Shi, Wei-Yi; He, Kuang; Wu, Yue-Yue; Wu, Wen-Xin; Lu, Jun-Hou; Song, Yu; Qu, Chen-Jiang; Wu, Hua
  作者单位：Soochow University - China; Soochow University - China; Soochow University - China; Soochow University - China; Soochow University - China
  摘要：Kirsten rat sarcoma virus (KRAS) mutation is associated with malignant tumor transformation and drug resistance. However, the development of clinically effective targeted therapies for KRAS-mutant cancer has proven to be a formidable challenge. Here, we report that tripartite motif- containing protein 21 (TRIM21) functions as a target of extracellular signal- regulated kinase 2 (ERK2) in KRAS- mutant colorectal cancer (CRC), contributing to regorafenib therapy resistance. Mechanistically, TRIM...
• Deep silicification-assisted long- term preservation of structural and genomic information across biospecies: From micro to macro AI翻译
  作者：Liang, Ke; Zhou, Liang; Lei, Qi; Ruan, Ting; Zhang, Minmin; Guo, Jimin; Brinker, C. Jeffrey; Zhu, Wei
  作者单位：South China University of Technology; Guangzhou Medical University; South China Normal University; Beijing University of Chemical Technology; University of New Mexico
  摘要：The concurrent preservation of morphological, structural, and genomic attributes within biological samples is paramount for comprehensive insights into biological phenomena and disease mechanisms. However, current preservation methodologies (e.g., cryopreservation, chemical reagent fixation, and bioplasticization) exhibit limitations in simultaneously achieving these critical combined goals. To address this gap, inspired by natural fossilization, here we propose deep silicification, a room tem...
• Estimates of actual and potential lives saved in the United States from the use of COVID-19 convalescent plasma AI翻译
  作者：Dragotakes, Quigly; Johnson, Patrick W.; Buras, Matthew R.; Carter, Rickey E.; Joyner, Michael J.; Bloch, Evan M.; Gebo, Kelly A.; Hanley, Daniel F.; Henderson, Jeffrey P.; Pirofski, Liise-Anne; Shoham, Shmuel; Senefeld, Jonathon W.; Tobian, Aaron A. R.; Wiggins, Chad C.; Wright, R. Scott; Paneth, Nigel S.; Sullivan, David J.; Casadevall, Arturo
  作者单位：Johns Hopkins University; Johns Hopkins Bloomberg School of Public Health; Mayo Clinic; Mayo Clinic; Mayo Clinic; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Washington University (WUSTL); Yeshiva University; University of Illinois System; University of Illinois Urbana-Champaign; Michigan State University; Mayo Clinic; Mayo Clinic; Michigan State University; Michigan State University
  摘要：In the Spring of 2020, the United States of America (USA) deployed COVID-19 convalescent plasma (CCP) to treat hospitalized patients. Over 500,000 patients were treated with CCP during the first year of the pandemic. In this study, we estimated the number of actual inpatient lives saved by CCP treatment in the United States of America based on CCP weekly use, weekly national mortality data, and CCP mortality reduction data from meta-analyses of randomized controlled trials and real-world data....
• Memory effects of transcription regulator-DNA interactions in bacteria AI翻译
  作者：Jung, Won; Chen, Tai-Yen; Santiago, Ace George; Chen, Peng
  作者单位：Cornell University; Harvard University; University of Houston System; University of Houston
  摘要：Memory effect refers to the phenomenon where past events influence a system's current and future states or behaviors. In biology, memory effects often arise from intra- or intermolecular interactions, leading to temporally correlated behaviors. Single-molecule studies have shown that enzymes and DNA-binding proteins can exhibit time-correlated behaviors of their activity. While memory effects are well documented and studied in vitro, no such examples exist in cells to our knowledge. Combining ...