2024

最新文章

• Working- age mortality is still an important driver of stagnating life expectancy in the United States AI翻译
  作者：Polizzi, Antonino; Dowd, Jennifer Beam
  作者单位：University of Oxford; University of Oxford; University of Oxford; University of Oxford
• A TLR4/TRAF6-dependent signaling pathway mediates NCoR coactivator complex formation for inflammatory gene activation AI翻译
  作者：Abe, Yohei; Kofman, Eric R.; Ouyang, Zhengyu; Cruz-Becerra, Grisel; Spann, Nathanael J.; Seidman, Jason S.; Troutman, Ty D.; Stender, Joshua D.; Taylor, Havilah; Fan, Weiwei; Link, Verena M.; Shen, Zeyang; Sakai, Juro; Downes, Michael; Evans, Ronald M.; Kadonaga, James T.; Rosenfeld, Michael G.; Glass, Christopher K.
  作者单位：University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; University System of Ohio; University of Cincinnati; Cincinnati Children's Hospital Medical Center; University of California System; University of California San Diego; University of California System; University of California San Diego; Salk Institute; University of Munich; University of California System; University of California San Diego; University of Tokyo; Tohoku University
  摘要：The nuclear receptor corepressor (NCoR) forms a complex with histone deacetylase 3 (HDAC3) that mediates repressive functions of unliganded nuclear receptors and other transcriptional repressors by deacetylation of histone substrates. Recent studies provide evidence that NCoR/HDAC3 complexes can also exert coactivator functions in brown adipocytes by deacetylating and activating PPAR gamma coactivator 1 alpha (PGC1 alpha) and that signaling via receptor activator of nuclear factor kappa -B (RA...
• Harnessing non- Watson-Crick's base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cells AI翻译
  作者：Gao, Shuliang; Guan, Huiwen; Bloomer, Hanan; Wich, Douglas; Song, Donghui; Khirallah, Jennifer; Ye, Zhongfeng; Zhao, Yu; Chen, Mengting; Xu, Chutian; Liu, Lihan; Xu, Qiaobing
  作者单位：Tufts University
  摘要：Genomic DNA of the cyanophage S -2L virus is composed of 2- aminoadenine (Z), thymine (T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates Watson-Crick base pairing rules. The Z -base has an extra amino group on the two position that allows the formation of a third hydrogen bond with thymine in DNA strands. Here, we explored and expanded applications of this non- Watson-Crick base pairing in protein expression and gene editing. Both ZTGC-DNA (Z -DNA) and ZUGC-R...
• A tiny fraction of all species forms most of nature: Rarity as a sticky state AI翻译
  作者：van Nes, Egbert H.; Pujoni, Diego G. F.; Shetty, Sudarshan A.; Straatsma, Gerben; de Vos, Willem M.; Scheffer, Marten
  作者单位：Wageningen University & Research; Universidade Federal de Minas Gerais; Wageningen University & Research; University of Helsinki
  摘要：Using data from a wide range of natural communities including the human micro -biome, plants, fish, mushrooms, rodents, beetles, and trees, we show that universally just a few percent of the species account for most of the biomass. This is in line with the classical observation that the vast bulk of biodiversity is very rare. Attempts to find traits allowing the tiny fraction of abundant species to escape rarity have remained unsuccessful. Here, we argue that this might be explained by the fac...
• Following the dynamics of industrial catalysts under operando conditions AI翻译
  作者：Van Speybroeck, Veronique
  作者单位：Ghent University
• Gaze into social bonds: Unlocking great ape emotions through eye- tracking AI翻译
  作者：Kret, Mariska E.
  作者单位：Leiden University - Excl LUMC; Leiden University
• Genome- wide and targeted CRISPR screens identify RNF213 as a mediator of interferon gamma-dependent pathogen restriction in human cells AI翻译
  作者：Matta, Sumit K.; Kohio, Hinissan P.; Chandra, Pallavi; Brown, Adam; Doench, John G.; Philips, Jennifer A.; Ding, Siyuan; Sibley, L. David
  作者单位：Washington University (WUSTL); Washington University (WUSTL); Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute
  摘要：To define cellular immunity to the intracellular pathogen Toxoplasma gondii, we performed a genome- wide CRISPR loss- of- function screen to identify genes important for (interferon gamma) IFN-gamma -dependent growth restriction. We revealed a role for the tumor suppressor NF2/Merlin for maximum induction of Interferon Stimulated Genes (ISG), which are positively regulated by the transcription factor IRF- 1. We then performed an ISG- targeted CRISPR screen that identified the host E3 ubiquitin...
• A species' response to spatial climatic variation does not predict its response to climate change AI翻译
  作者：Perret, Daniel L.; Evans, Margaret E. K.; Sax, Dov F.
  作者单位：Brown University; University of Arizona; Brown University; United States Department of Agriculture (USDA); United States Forest Service; Oak Ridge Associated Universities; United States Department of Energy (DOE); Oak Ridge Institute for Science & Education
  摘要：The dominant paradigm for assessing ecological responses to climate change assumes that future states of individuals and populations can be predicted by current, species -wide performance variation across spatial climatic gradients. However, if the fates of ecological systems are better predicted by past responses to in situ climatic variation through time, this current analytical paradigm may be severely misleading. Empirically testing whether spatial or temporal climate responses better pred...
• Integrin- activating Yersinia protein Invasin sustains long- term expansion of primary epithelial cells as 2D organoid sheets AI翻译
  作者：Wijnakker, Joost J. A. P. M.; Son, Gijs J. F. van; Krueger, Daniel; Wetering, Willine J. van de; Iglesias, Carmen Lopez -; Schreurs, Robin; van Rijt, Fenna; Lim, Sangho; Lin, Lin; Peters, Peter J.; Isberg, Ralph R.; Janda, Claudia Y.; de Lau, Wim; Clevers, Hans
  作者单位：Royal Netherlands Academy of Arts & Sciences; Hubrecht Institute (KNAW); Utrecht University; Utrecht University Medical Center; Princess Maxima Center; Maastricht University; Tufts University; Roche Holding
  摘要：Matrigel (R)/BME (R), a basement membrane- like preparation, supports long- term growth of epithelial 3D organoids from adult stem cells [T. Sato et al., Nature 459, 262-265 (2009); T. Sato et al., Gastroenterology 141, 1762-1772 (2011)]. Here, we show that interaction between Matrigel's major component laminin-111 with epithelial alpha 6 beta 1- integrin is crucial for this process. The outer membrane protein Invasin of Yersinia is known to activate multiple integrin-beta 1 complexes, includi...
• Microscopic phage adsorption assay: High- throughput quantification of virus particle attachment to host bacterial cells AI翻译
  作者：Antani, Jyot D.; Ward, Timothy; Emonet, Thierry; Turner, Paul E.
  作者单位：Yale University; Yale University; Yale University; Yale University; Yale University; Yale University
  摘要：Phages, viruses of bacteria, play a pivotal role in Earth's biosphere and hold great promise as therapeutic and diagnostic tools in combating infectious diseases. Attachment of phages to bacterial cells is a crucial initial step of the interaction. The classic assay to quantify the dynamics of phage attachment involves coculturing and enumeration of bacteria and phages, which is laborious, lengthy, hence low- throughput, and only provides ensemble estimates of model- based adsorption rate cons...