-
作者：Yang, Aerin; Jude, Kevin M.; Lai, Ben; Minot, Mason; Kocyla, Anna M.; Glassman, Caleb R.; Nishimiya, Daisuke; Kim, Yoon Seok; Reddy, Sai T.; Khan, Aly A.; Garcia, K. Christopher
作者单位：Stanford University; Howard Hughes Medical Institute; Stanford University; Toyota Technological Institute - Chicago; Swiss Federal Institutes of Technology Domain; ETH Zurich; University of Chicago; University of Chicago; Stanford University
摘要：Fine-tuning of protein-protein interactions occurs naturally through coevolution, but this process is difficult to recapitulate in the laboratory. We describe a platform for synthetic protein-protein coevolution that can isolate matched pairs of interacting muteins from complex libraries. This large dataset of coevolved complexes drove a systems-level analysis of molecular recognition between Z domain-affibody pairs spanning a wide range of structures, affinities, cross-reactivities, and ortho...
-
作者：Popkin, Gabriel
-
作者：Cheng, Lei; Li, Dian; Mai, Binh Khanh; Bo, Zhiyu; Cheng, Lida; Liu, Peng; Yang, Yang
作者单位：University of California System; University of California Santa Barbara; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; University of California System; University of California Santa Barbara
摘要：Developing synthetically useful enzymatic reactions that are not known in biochemistry and organic chemistry is an important challenge in biocatalysis. Through the synergistic merger of photoredox catalysis and pyridoxal 5'-phosphate (PLP) biocatalysis, we developed a pyridoxal radical biocatalysis approach to prepare valuable noncanonical amino acids, including those bearing a stereochemical dyad or triad, without the need for protecting groups. Using engineered PLP enzymes, either enantiomer...
-
作者：Cohen, Jon
-
作者：Mervis, Jeffrey
-
作者：Ziegler, Karin A.; Ahles, Andrea; Dueck, Anne; Esfandyari, Dena; Pichler, Pauline; Weber, Karolin; Kotschi, Stefan; Bartelt, Alexander; Sinicina, Inga; Graw, Matthias; Leonhardt, Heinrich; Weckbach, Ludwig T.; Massberg, Steffen; Schifferer, Martina; Simons, Mikael; Hoeher, Luciano; Luo, Jie; Ertuerk, Ali; Schiattarella, Gabriele G.; Sassi, Yassine; Misgeld, Thomas; Engelhardt, Stefan
作者单位：Technical University of Munich; Munich Heart Alliance; German Centre for Cardiovascular Research; University of Munich; Helmholtz Association; Helmholtz-Center Munich - German Research Center for Environmental Health; Harvard University; Harvard T.H. Chan School of Public Health; University of Munich; University of Munich; University of Munich; University of Munich; Helmholtz Association; German Center for Neurodegenerative Diseases (DZNE); University of Munich; Technical University of Munich; Helmholtz Association; Helmholtz-Center Munich - German Research Center for Environmental Health; University of Munich; German Centre for Cardiovascular Research; Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; Helmholtz Association; Max Delbruck Center for Molecular Medicine; Virginia Polytechnic Institute & State University; Virginia Polytechnic Institute & State University
摘要：Disruption of the physiologic sleep-wake cycle and low melatonin levels frequently accompany cardiac disease, yet the underlying mechanism has remained enigmatic. Immunostaining of sympathetic axons in optically cleared pineal glands from humans and mice with cardiac disease revealed their substantial denervation compared with controls. Spatial, single-cell, nuclear, and bulk RNA sequencing traced this defect back to the superior cervical ganglia (SCG), which responded to cardiac disease with ...
-
作者：Lenda, Magdalena; Skorka, Piotr; Possingham, Hugh P.; Knops, Johannes M. H.
作者单位：Polish Academy of Sciences; University of Queensland; Xi'an Jiaotong-Liverpool University
-
作者：Zhang, Min; Jungblut, Anna; Kunert, Franziska; Hauptmann, Luis; Hoffmann, Thomas; Kolesnikova, Olga; Metzner, Felix; Moldt, Manuela; Weis, Felix; DiMaio, Frank; Hopfner, Karl-Peter; Eustermann, Sebastian
作者单位：European Molecular Biology Laboratory (EMBL); Ruprecht Karls University Heidelberg; University of Munich; University of Washington; University of Washington Seattle; Communaute Universite Grenoble Alpes; Universite Grenoble Alpes (UGA); CEA; Centre National de la Recherche Scientifique (CNRS)
摘要：Loss of H2A-H2B histone dimers is a hallmark of actively transcribed genes, but how the cellular machinery functions in the context of noncanonical nucleosomal particles remains largely elusive. In this work, we report the structural mechanism for adenosine 5-triphosphate-dependent chromatin remodeling of hexasomes by the INO80 complex. We show how INO80 recognizes noncanonical DNA and histone features of hexasomes that emerge from the loss of H2A-H2B. A large structural rearrangement switches...
-
作者：Anderson, Eric; Fan, Feng-Ren; Cai, Jiaqi; Holtzmann, William; Taniguchi, Takashi; Watanabe, Kenji; Xiao, Di; Yao, Wang; Xu, Xiaodong
作者单位：University of Washington; University of Washington Seattle; University of Hong Kong; National Institute for Materials Science; National Institute for Materials Science; University of Washington; University of Washington Seattle
摘要：The ability to control the underlying lattice geometry of a system may enable transitions between emergent quantum ground states. We report in situ gate switching between honeycomb and triangular lattice geometries of an electron many-body Hamiltonian in rhombohedral (R)-stacked molybdenum ditelluride (MoTe2) moire bilayers, resulting in switchable magnetic exchange interactions. At zero electric field, we observed a correlated ferromagnetic insulator near one hole per moire unit cell with a w...
-
作者：Stokstad, Erik
