6748期

最新文章

• AI-designed antibody candidates hit a crucial target AI翻译
  作者：Service, Robert F.
• Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase AI翻译
  作者：Witte, Isaac P.; Lampe, George D.; Eitzinger, Simon; Miller, Shannon M.; Berrios, Kiara N.; McElroy, Amber N.; King, Rebeca T.; Stringham, Olivia G.; Gelsinger, Diego R.; Vo, Phuc Leo H.; Chen, Albert T.; Tolar, Jakub; Osborn, Mark J.; Sternberg, Samuel H.; Liu, David R.
  作者单位：Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Howard Hughes Medical Institute; Harvard University; Columbia University; Howard Hughes Medical Institute; Columbia University; University of Minnesota System; University of Minnesota Twin Cities; Scripps Research Institute; Vertex Pharmaceuticals
  摘要：Programmable gene integration in human cells has the potential to enable mutation-agnostic treatments for loss-of-function genetic diseases and facilitate many applications in the life sciences. CRISPR-associated transposases (CASTs) catalyze RNA-guided DNA integration but thus far demonstrate minimal activity in human cells. Using phage-assisted continuous evolution (PACE), we generated CAST variants with >200-fold average improved integration activity. The evolved CAST system (evoCAST) achie...
• Executive order on risky research brings confusion AI翻译
  作者：Kupferschmidt, Kai
• Sustainability insights from Late Pleistocene climate change and horse migration patterns AI翻译
  作者：Bataille, Clement P.; Hershauer, Samantha; Justin, Wilson; Stelkia, James Aaron; Topkok, Sean Asikluk; Leonard, Beth Ginondidoy; Means, Bill; Koskey, Michael; Kapp, Joshua D.; Landry, Zoe; Fraser, Danielle; Southon, John; Lindroos, Eve E.; Hassler, Auguste; Chauvey, Lorelei; Tressieres, Gaetan; Tonasso-Calviere, Laure; Schiavinato, Stephanie; Seguin-Orlando, Andaine; Perdereau, Aude; Oliveira, Pedro H.; Aury, Jean-Marc; Wincker, Patrick; Kirillova, Irina V.; Vasiliev, Sergey K.; Kusliy, Mariya A.; Graphodatsky, Alexander S.; Tishkin, Alexey A.; Barnes, Ian; Druckenmiller, Pat; Jass, Christopher N.; Macphee, Ross D. E.; Barron-Ortiz, Christina I.; Groves, Pam; Mann, Dan; Froese, Duane G.; Wooller, Matthew; Miller, Joshua H.; Crowley, Brooke; Zazula, Grant; Hall, Elizabeth; Hewitson, Susan; Shapiro, Beth; Orlando, Ludovic
  作者单位：Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Ecology & Environment (INEE); Universite de Toulouse; Universite Toulouse III - Paul Sabatier; Universites de Strasbourg Etablissements Associes; Universite de Strasbourg; University of Ottawa; Purdue University System; Purdue University; University of California System; University of California Santa Cruz; University of Alaska System; University of Alaska Fairbanks; Alaska Pacific University; University of California System; University of California Santa Cruz; Carleton University; Smithsonian Institution; Smithsonian National Museum of Natural History; University of California System; University of California Irvine; University of Aberdeen; CEA; Universite Paris Saclay; CEA; Universite Paris Saclay; Russian Academy of Sciences; Siberian Branch of the Russian Academy of Sciences; Altai State University; Natural History Museum London; University of Alaska System; University of Alaska Fairbanks; University of Alaska System; University of Alaska Fairbanks; American Museum of Natural History (AMNH); University of Alaska System; University of Alaska Fairbanks; University of Alberta; University of Alaska System; University of Alaska Fairbanks; University System of Ohio; University of Cincinnati; University System of Ohio; University of Cincinnati
  摘要：Climate affects habitat, food availability, and the movement and sustainability of all life. In this work, we apply Indigenous and Western scientific methods, including genomics and isotope profiling, on fossils from across Beringia to explore the effect of climate change on horses. We find that Late Pleistocene horses from Alaska and northern Yukon are related to populations from Eurasia and crossed the Bering land bridge multiple times during the last glacial interval. We also find deeply di...
• Leaving synthetic pesticides behind AI翻译
  作者：Wan, Nian-Feng; Woodcock, Ben A.; Scherber, Christoph; Wyckhuys, Kris A. G.; Li, Zhong; Qian, Xuhong
  作者单位：East China University of Science & Technology; UK Centre for Ecology & Hydrology (UKCEH); Zoologisches Forschungsmuseum Alexander Koenig (ZFMK); University of Bonn; Chinese Academy of Agricultural Sciences; Institute of Plant Protection, CAAS; University of Queensland; East China Normal University
• Norepinephrine changes behavioral state through astroglial purinergic signaling AI翻译
  作者：Chen, Alex B.; Duque, Marc; Rymbek, Altyn; Dhanasekar, Mahalakshmi; Wang, Vickie M.; Mi, Xuelong; Tocquer, Loeva; Narayan, Sujatha; Legorreta, Emmanuel Marquez; Eddison, Mark; Yu, Guoqiang; Wyart, Claire; Prober, David A.; Engert, Florian; Ahrens, Misha B.
  作者单位：Howard Hughes Medical Institute; Harvard University; Harvard University; Harvard Medical School; California Institute of Technology; Assistance Publique Hopitaux Paris (APHP); Institut National de la Sante et de la Recherche Medicale (Inserm); Sorbonne Universite; Virginia Polytechnic Institute & State University; Tsinghua University; Allen Institute for Brain Science
  摘要：Both neurons and glia communicate through diffusible neuromodulators; however, how neuron-glial interactions in such neuromodulatory networks influence circuit computation and behavior is unclear. During futility-induced behavioral transitions in the larval zebrafish, the neuromodulator norepinephrine (NE) drives fast excitation and delayed inhibition of behavior and circuit activity. We found that astroglial purinergic signaling implements the inhibitory arm of this motif. In larval zebrafish...
• Energy's History: Toward a Global Canon AI翻译
  作者：Sorkhabi, Rasoul
  作者单位：Utah System of Higher Education; University of Utah
• Distribution of haploid chromosomes into separate nuclei in two pathogenic fungi AI翻译
  作者：Xu, Yan; Tian, Lei; Tan, Jinyi; Huang, Weijie; Li, Josh; O'Neil, Nigel; Hirst, Martin; Hieter, Phil; Zhang, Yuelin; Li, Xin
  作者单位：Sichuan University; University of British Columbia; University of British Columbia
  摘要：Nuclei define eukaryotes, enabling macromolecular compartmentalization and cellular regulation. Each nucleus is believed to contain one or more haploid sets of chromosomes (1N). However, we discovered that haploid cells of the pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea distribute their chromosomes such that each of their nuclei contains only a subset of the haploid chromosomes (<= 1/2N). The unusual chromosomal distribution was confirmed by cellular and molecular methods in...
• Revolutionize textile recycling AI翻译
  作者：Dai, Leilei; Ruan, Roger; Lei, Hanwu; Xiao, Rui; Zhang, Huiyan
  作者单位：Southeast University - China; University of Minnesota System; University of Minnesota Twin Cities; Washington State University
• GPCR signaling gates astrocyte responsiveness to neurotransmitters and control of neuronal activity AI翻译
  作者：Guttenplan, Kevin A.; Maxwell, Isa; Santos, Erin; Borchardt, Luke A.; Manzo, Ernesto; Abalde-Atristain, Leire; Kim, Rachel D.; Freeman, Marc R.
  作者单位：Oregon Health & Science University; New York University
  摘要：How astrocytes regulate neuronal circuits is a fundamental question in neurobiology. Specifically, how astrocytes respond to different neurotransmitters in vivo and how they affect downstream circuit modulation are questions that remain to be fully elucidated. Here, we report a mechanism in Drosophila by which G protein-coupled adrenergic signaling in astrocytes can control-or gate-their ability to respond to other neurotransmitters. Further, we show that manipulating this pathway potently reg...