6752期

最新文章

• Evolutionary-scale enzymology enables exploration of a rugged catalytic landscape AI翻译
  作者：Muir, Duncan F.; Asper, Garrison P. R.; Notin, Pascal; Posner, Jacob A.; Marks, Debora S.; Keiser, Michael J.; Pinney, Margaux M.
  作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco; Harvard University; Harvard Medical School; University of Oxford; California State University System; San Francisco State University; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California Berkeley
  摘要：Quantitatively mapping enzyme sequence-catalysis landscapes remains a critical challenge in understanding enzyme function, evolution, and design. In this study, we leveraged emerging microfluidic technology to measure catalytic constants-kcat and KM-for hundreds of diverse orthologs and mutants of adenylate kinase (ADK). We dissected this sequence-catalysis landscape's topology, navigability, and mechanistic underpinnings, revealing catalytically heterogeneous neighborhoods organized by domain...
• Cat1 forms filament networks to degrade NAD+ during the type III CRISPR-Cas antiviral response AI翻译
  作者：Baca, Christian F.; Majumder, Puja; Hickling, James H.; Patel, Dinshaw J.; Marraffini, Luciano A.
  作者单位：Rockefeller University; Cornell University; Weill Cornell Medicine; Rockefeller University; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Howard Hughes Medical Institute; Rockefeller University
  摘要：Type III CRISPR-Cas systems defend against viral infection in prokaryotes by using an RNA-guided complex that recognizes foreign transcripts and synthesizes cyclic oligoadenylate (cOA) messengers to activate CRISPR-associated Rossmann-fold (CARF) immune effectors. In this study, we investigated a protein containing a CARF domain-fused Toll/interleukin-1 receptor (TIR) domain, Cat1. We found that Cat1 provides immunity by cleaving and depleting oxidized nicotinamide adenine dinucleotide (NAD+) ...
• Bacteria poison and eat their neighbors AI翻译
  作者：Nadell, Carey D.; Marx, Christopher J.
  作者单位：Dartmouth College; Dartmouth College; University of Idaho
• The Underland AI翻译
  作者：Dominy, Nathaniel J.
  作者单位：Dartmouth College; Dartmouth College
• Minute-scale dynamics of recurrent dike intrusions in Iceland with fiber-optic geodesy AI翻译
  作者：Li, Jiaxuan; Biondi, Ettore; Heimisson, Elias Rafn; Puel, Simone; Zhai, Qiushi; Zhang, Shane; Hjorleifsdottir, Vala; Wei, Xiaozhuo; Bird, Elijah; Klesh, Andy; Kamalov, Valey; Gunnarsson, Theodor; Geirsson, Halldor; Zhan, Zhongwen
  作者单位：California Institute of Technology; University of Houston System; University of Houston; University of Iceland; Reykjavik University; National Aeronautics & Space Administration (NASA); NASA Jet Propulsion Laboratory (JPL); Alphabet Inc.; Google Incorporated
  摘要：Continuous geodetic measurements near volcanic systems can image magma transport dynamics, yet resolving dike intrusions with high spatiotemporal resolution remains challenging. We introduce fiber-optic geodesy, leveraging low-frequency distributed acoustic sensing (LFDAS) recordings along a telecommunication fiber-optic cable, to track dike intrusions near Grindav & iacute;k, Iceland, on a minute timescale. LFDAS revealed distinct strain responses from nine intrusive events, six resulting in ...
• Proposed LIGO cut could snuff out brand new form of astronomy AI翻译
  作者：Cho, Adrian
• Many scientific societies are losing publishing revenue AI翻译
  作者：Brainard, Jeffrey
• Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing AI翻译
  作者：Sexton, Zachary A.; Rutsche, Dominic; Herrmann, Jessica E.; Hudson, Andrew R.; Sinha, Soham; Du, Jianyi; Shiwarski, Daniel J.; Masaltseva, Anastasiia; Solberg, Fredrik Samdal; Pham, Jonathan; Szafron, Jason M.; Wu, Sean M.; Feinberg, Adam W.; Skylar-Scott, Mark A.; Marsden, Alison L.
  作者单位：Stanford University; Stanford University; Stanford University; Carnegie Mellon University; Stanford University; Stanford University; Stanford University; Carnegie Mellon University; Stanford University; Chan Zuckerberg Initiative (CZI); Stanford University
  摘要：Our ability to produce human-scale biomanufactured organs is limited by inadequate vascularization and perfusion. For arbitrarily complex geometries, designing and printing vasculature capable of adequate perfusion poses a major hurdle. We introduce a model-driven design platform that demonstrates rapid synthetic vascular model generation alongside multifidelity computational fluid dynamics simulations and three-dimensional bioprinting. Key algorithmic advances accelerate vascular generation 2...
• US tariffs jeopardize medicine supply chains AI翻译
  作者：Casiraghi, Giona; Andres, Georges; Schweitzer, Frank; Verginer, Luca
  作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich
• La- and Mn-doped cobalt spinel oxygen evolution catalyst for proton exchange membrane electrolysis (vol. 380, pg. 609, 2023) AI翻译
  作者：Chong, L.