Cat1 forms filament networks to degrade NAD+ during the type III CRISPR-Cas antiviral response

• 成果类型: Article
• 署名作者: Baca, Christian F.; Majumder, Puja; Hickling, James H.; Patel, Dinshaw J.; Marraffini, Luciano A.
• 署名单位: Rockefeller University; Cornell University; Weill Cornell Medicine; Rockefeller University; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Howard Hughes Medical Institute; Rockefeller University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10105
• DOI: 10.1126/science.adv9045
• 发表日期: 2025-06-12
• 关键词: immune-system; rna cleavage; tir domains; cell-death; dna; DEFENSE; protein; gene

摘要

Type III CRISPR-Cas systems defend against viral infection in prokaryotes by using an RNA-guided complex that recognizes foreign transcripts and synthesizes cyclic oligoadenylate (cOA) messengers to activate CRISPR-associated Rossmann-fold (CARF) immune effectors. In this study, we investigated a protein containing a CARF domain-fused Toll/interleukin-1 receptor (TIR) domain, Cat1. We found that Cat1 provides immunity by cleaving and depleting oxidized nicotinamide adenine dinucleotide (NAD+) molecules from the infected host, inducing a growth arrest that prevents viral propagation. Cat1 forms dimers that stack upon each other to generate long filaments that are maintained by bound cOA ligands, with stacked TIR domains forming the NAD+ cleavage catalytic sites. Furthermore, Cat1 filaments assemble into distinct trigonal and pentagonal networks that enhance NAD+ degradation. Cat1 presents an unprecedented chemistry and higher-order protein assembly for the CRISPR-Cas response.