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作者：Shen, Zhuofan; Khare, Sagar D.
作者单位：Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick
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作者：Tang, Stephen; Zedaveinyte, Rimante; Burman, Nathaniel; Pandey, Shishir; Ramirez, Josephine L.; Kulber, Louie M.; Wiegand, Tanner; Wilkinson, Royce A.; Ma, Yanzhe; Zhang, Dennis J.; Lampe, George D.; Berisa, Mirela; Jovanovic, Marko; Wiedenheft, Blake; Sternberg, Samuel H.
作者单位：Columbia University; Montana State University System; Montana State University Bozeman; Howard Hughes Medical Institute; Columbia University; Columbia University; Icahn School of Medicine at Mount Sinai; University of Copenhagen
摘要：Bacteria defend themselves from viral predation using diverse immune systems, many of which target foreign DNA for degradation1. Defence-associated reverse transcriptase (DRT) systems provide an intriguing counterpoint to this strategy by using DNA synthesis instead2,3. We and others recently showed that DRT2 systems use an RNA template to assemble a de novo gene that encodes the antiviral effector protein Neo4,5. It remains unclear whether similar mechanisms of defence are used by other relat...
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作者：Lenharo, Mariana
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作者：Kapadia, Nitin; Nurse, Paul
作者单位：Francis Crick Institute; Rockefeller University
摘要：Mitotic onset is a critical transition for eukaryotic cell proliferation. The commonly held view of mitotic control is that the master regulator, cyclin-dependent kinase (CDK), is first activated in the cytoplasm, at the centrosome, initiating mitosis1, 2-3. Bistability in CDK activation ensures that the transition is irreversible, but how this unfolds in a spatially compartmentalized cell is unknown4, 5, 6, 7-8. Here, using fission yeast, we show that CDK is first activated in the nucleus, an...
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作者：[Anonymous]
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作者：Mao, Kaining; Liu, Chenfei; Wang, Yi; Gu, Chaoxuan; Putziger, John M.; Cemalovic, Nicholas I.; Muniz, Cameron; Qi, Yue; Lin, Song
作者单位：Cornell University; Brown University
摘要：The synthesis of enantiopure compounds is a central focus in organic chemistry owing to the prevalence of chiral centres in biological systems and the impact of homochirality on molecular properties. With growing recognition of electrochemistry as a powerful tool to improve the scope and sustainability of organic synthesis1, increasing efforts have been directed towards developing asymmetric electrocatalytic reactions to access challenging chiral molecules2, 3-4. However, many useful electroch...
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作者：Sakoguchi, Akihito; Chamberlain, Samuel G.; Morch, Alexander M.; Widdess, Marcus; Harrison, Thomas E.; Dustin, Michael L.; Arase, Hisashi; Higgins, Matthew K.; Iwanaga, Shiroh
作者单位：University of Osaka; University of Oxford; University of Oxford; University of Oxford; Kennedy Institute for Rheumatology; Chinese Academy of Medical Sciences - Peking Union Medical College; University of Oxford; University of Osaka; University of Osaka; University of Osaka; University of Osaka
摘要：Natural killer (NK) cells use inhibitory and activating immune receptors to differentiate between human cells and pathogens. Signalling by these receptors determines whether an NK cell becomes activated and destroys a target cell. In some cases, such as killer immunoglobulin-like receptors, immune receptors are found in pairs, with inhibitory and activating receptors containing nearly identical extracellular ligand-binding domains coupled to different intracellular signalling domains1. Previou...
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作者：[Anonymous]
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作者：Liu, Jiangle; Yan, Xueqing; Wu, Hao; Ji, Ziqin; Shan, Ye; Wang, Xinyan; Ran, Yunfan; Ma, Yichen; Li, Caitao; Zhu, Yuchao; Gu, Ruichu; Wen, Han; Yi, Chengqi; Chen, Peng R.
作者单位：Peking University; Peking University; Peking University; Shanghai Jiao Tong University; ShanghaiTech University; Peking University; Peking University
摘要：The incorporation of non-canonical amino acids (ncAAs) enables customized chemistry to tailor protein functions1, 2-3. Genetic code expansion offers a general approach for ncAA encoding by reassigning stop codons as the 'blank' codon; however, it is not completely orthogonal to translation termination for cellular transcripts. Here, to generate more bona fide blank codons, we developed an RNA codon-expansion (RCE) strategy that introduces and decodes bioorthogonally assignable pseudouridine (P...
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作者：Naddaf, Miryam
