47期

最新文章

• Creatine kinase imaging (CKI) for in vivo whole-brain mapping of creatine kinase reaction kinetics AI翻译
  作者：Widmaier, Mark; Kaiser, Antonia; Pandurevic, Pontus; Lim, Song-, I; Doring, Andre; Huang, Zhiwei; Wenz, Daniel; Xiao, Ying; Jiang, Yun; Lin, Yan; Xin, Lijing
  作者单位：Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; University of Michigan System; University of Michigan; Shantou University; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne
  摘要：The creatine kinase (CK) is a key enzyme involved in brain bioenergetics, playing an important role in brain function and the pathogenesis of neurological and psychiatric diseases and cancers. However, imaging its activity noninvasively in the human brain remains a significant challenge. This study aims to advance 31P magnetic resonance fingerprinting for Creatine Kinase Imaging (CKI). The method was implemented and validated on a clinical 7 Tesla MRI scanner. CKI enables whole-brain mapping o...
• Biodiversity conservation requires consideration of different life history stages AI翻译
  作者：Song, Yan-Fang; Liu, Xiao-Long; Liu, Jia-Jia; Li, Qing-Qing; Li, Xian-Qi; Lu, Wen-Zhu; Zhou, Wei-Wei; Yuan, Zhi-Yong
  作者单位：Southwest University - China; Lanzhou University; Lanzhou University; Southwest Forestry University - China; Fudan University
  摘要：Amid the biodiversity crisis, functional diversity, which is critical for ecosystem stability and conservation planning-faces challenges due to species' complex life cycles. As the majority of animals, species with complex life histories may have distinct traits and ecological functions at different stages. Using all 557 Chinese anurans, we tested whether adult-based conservation protects entire taxa by analyzing functional trait diversity across life stages. We compiled adult and tadpole trai...
• The adhesion GPCR ADGRL2 engages Gα13 to enable epidermal differentiation AI翻译
  作者：Yang, Xue; He, Feng; Lopez-Pajares, Vanessa; Porter, Douglas F.; Garbett, Krassimira; Siprashvili, Zurab; Ducoli, Luca; Meyers, Robin M.; Reynolds, David L.; Bui, Duy Lan Huong; Hong, Audrey; Nguyen, Duy Thanh; Jing, Yuqing; Mondal, Smarajit; Ko, Lisa; Tao, Shiying; Singal, Bharti; Sando, Richard; Skiniotis, Georgios; Khavari, Paul A.
  作者单位：Stanford University; Stanford Medicine; Stanford University; Stanford University; Stanford Medicine; Vanderbilt University; Stanford University; Stanford Medicine; Stanford Medicine; Stanford University; Stanford University; Stanford Medicine; US Department of Veterans Affairs; Veterans Health Administration (VHA); VA Palo Alto Health Care System; St Jude Children's Research Hospital; St Jude Children's Research Hospital
  摘要：Homeostasis relies on signaling networks controlled by cell membrane receptors. Although G-protein-coupled receptors (GPCRs) are the largest family of transmembrane receptors, their specific roles in the epidermis are not fully understood. Dual CRISPR-Flow and single cell Perturb RNA-sequencing knockout screens of all epidermal GPCRs were thus performed, uncovering an essential requirement for adhesion GPCR ADGRL2 (latrophilin 2) in epidermal differentiation. Among potential downstream guanine...
• Marine heatwave decimates fire coral populations in the Caribbean AI翻译
  作者：Dell'Antonio, Emilia C.; Mahoney, Lauren; Edmunds, Peter J.
  作者单位：California State University System; California State University Northridge
  摘要：Marine heatwaves (MHW) are common destructive events affecting coral reefs. After decades of degradation, the shallow reefs of the United States Virgin Islands have been depleted of scleractinian corals, leaving abundant colonies of the hydrozoan fire coral Millepora dominating the coral community. This dominance ended in 2024 after 84% of Millepora colonies over 43 km of shore were killed by a MHW that brought the hottest October in the 36 y since monitoring began. In August 2024, dead Millep...
• The immunoproteasome regulates ILC2 responses mitochondrial AI翻译
  作者：Laurent, Paoline; Chaudhary, Vidyanath; Li, Daqiang; Kioon, Marie Dominique Ah; Zhang, Chen; Miller, William H.; Liao, Hua; Lin, Gang; Nathan, Carl F.; Barrat, Franck J.
  作者单位：Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine; Cornell University
  摘要：Type 2 innate lymphoid cells (ILC2s) contribute to type 2 immunity but have also been associated with multiple inflammatory diseases, including airway inflammation and asthma. We report that beyond its function of degrading poly-ubiquitinylated proteins, the immunoproteasome (i-20S) is required for the proper function of ILC2s by controlling their mitochondrial capacity. We found that 90% of the catalytic beta subunits of proteasomes in human ILC2s (hILC2s) are the immuno-(beta 5i) rather than...
• The telomeric valine-arginine dipeptide repeat protein changes state to diffuse staining in mitosis and represses in vitro translation AI翻译
  作者：Al-Turki, Taghreed; Mantri, Venkata; Willcox, Smaranda; Mills, C. Allie; Herring, Laura E.; Cho, Su-Ji; Lee, Hannah; Meyer, Caliyn; Anton, E. S.; Griffith, Jack D.
  作者单位：University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine
  摘要：Translation of mammalian G-rich telomeric RNA via the Repeat Associated non-AUG (AUG, the mRNA start codon) mechanism can produce proteins consisting of long repeats of valine-arginine (VR) or glycine-leucine (GL) dipeptides. Their role in the cell has not been elucidated. Using confocal laser scanning microscopy combined with antibody staining we previously observed VR sequestered in punctate bodies and liquid droplets in the cytoplasm and nuclei of nonmitotic cells. Here, we report that cell...
• High- throughput screening for class I peptide MHC binding via yeast surface display AI翻译
  作者：Holec, Patrick, V; Breuckman, Kathryn C.; Leddy, Owen; White, Forest M.; Bryson, Bryan D.; Birnbaum, Michael E.
  作者单位：Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Ragon Institute; Harvard University Medical Affiliates; Massachusetts General Hospital
  摘要：T cells rely on short peptides presented by highly polymorphic major histocompatibility complexes (MHCs) to selectively initiate adaptive immune responses. Despite its importance, few techniques can systematically evaluate stable peptide presentation across diverse MHC alleles. Here, we describe a yeast display pipeline that can be deployed to rapidly screen peptides to identify class I pMHC binders across many alleles. Through this, we isolate unique biological phenomena such as alteration of...
• Glyceraldehyde-3-phosphate dehydrogenase homologs as bifunctional gatekeepers of metabolic segregation in Pseudomonas putida AI翻译
  作者：Zhou, Nanqing; Mendonca, Caroll M.; Carroll, Austin L.; Pate, Stefan; Nieto-Dominguez, Manuel; Chen, Xinyu; Zhang, Lichun; Teitel, Kelly P.; Dekker, Nienke K.; Elmore, Joshua R.; Nikel, Pablo I.; Waldbauer, Jacob R.; Guss, Adam M.; Mangan, Niall M.; Aristilde, Ludmilla
  作者单位：Northwestern University; Cornell University; United States Department of Energy (DOE); Oak Ridge National Laboratory; Northwestern University; Technical University of Denmark; University of Chicago; Northwestern University; National Science Foundation (NSF); Northwestern University
  摘要：Metabolically versatile Pseudomonas species can assimilate various glycolytic and gluconeogenic substrates. Simultaneous assimilation is known to segregate carbons from each substrate type into different metabolic pathways. However, the mechanisms of this metabolic segregation remain unresolved. Here, we investigate Pseudomonas putida KT2440 during processing of the sugar glucose through glycolysis versus the phenolic acid ferulate through gluconeogenesis. Metabolome profiling reveals up to tw...
• Coordinated transfer of DNA between Pol θ and Pol δ resets microhomology choice during double- strand break repair AI翻译
  作者：Li, Yuzhen; Returan, Mark; Guerin, Adele T.; Averill, April M.; Oladapo, Dorcas; Doublie, Sylvie; Wood, Richard D.
  作者单位：University of Texas System; UTMD Anderson Cancer Center; University of Vermont
  摘要：DNA polymerase theta (Pol 0)-mediated end joining (TMEJ) initiates DNA double-strand break repair by using short homologies (microhomologies) between single-stranded DNA tails. This repair process is particularly important in cancer cells defective in homologous recombination. The exonuclease function of DNA polymerase delta (Pol S) has been identified as an essential component for TMEJ, functioning to remove unpaired bases flanking a microhomology (MH). It is not known if the exonuclease remo...
• High-resolution single-cell analyses reveal evolutionary constraints and evolvability of sexual circuits in Drosophila AI翻译
  作者：Walsh, Justin T.; Junker, Ian P.; Chen, Yu- Chieh David; Chen, Yen - Chung; Gifford, Helena; Chen, Dawn S.; Ding, Yun
  作者单位：University of Pennsylvania; New York University; Vanderbilt University; Pennsylvania Commonwealth System of Higher Education (PCSHE); Temple University
  摘要：Understanding how the cellular and molecular composition of neural circuits evolves to generate species-specific behaviors remains a major challenge in evolutionary biology and neuroscience. The remarkable diversity of male sexual behaviors among Drosophila species, despite their recent divergence, offers an excellent model for addressing this question. Here, by harnessing single-cell transcriptomics of the sexual circuits labeled by the sex determination gene doublesex (dsx) at high resolutio...