Creatine kinase imaging (CKI) for in vivo whole-brain mapping of creatine kinase reaction kinetics

• 成果类型: Article
• 署名作者: Widmaier, Mark; Kaiser, Antonia; Pandurevic, Pontus; Lim, Song-, I; Doring, Andre; Huang, Zhiwei; Wenz, Daniel; Xiao, Ying; Jiang, Yun; Lin, Yan; Xin, Lijing
• 署名单位: Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; University of Michigan System; University of Michigan; Shantou University; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2505323122
• 发表日期: 2025-11-25
• 页码: e2505323122
• 关键词: creatine kinase; mri; P-31 MRS; brain; bioenergetics; P-31 MAGNETIZATION-TRANSFER; ABSOLUTE METABOLITE CONCENTRATIONS; REACTION-RATES; RESONANCE-SPECTROSCOPY; PROBABILISTIC ATLAS; energy-metabolism; skeletal-muscle; 7 T; saturation; cortex

摘要

The creatine kinase (CK) is a key enzyme involved in brain bioenergetics, playing an important role in brain function and the pathogenesis of neurological and psychiatric diseases and cancers. However, imaging its activity noninvasively in the human brain remains a significant challenge. This study aims to advance 31P magnetic resonance fingerprinting for Creatine Kinase Imaging (CKI). The method was implemented and validated on a clinical 7 Tesla MRI scanner. CKI enables whole-brain mapping of CK forward rate constant, revealing regional differences. Furthermore, a functional CKI (fCKI) study demonstrated a CK activation map in response to visual stimulation, revealing an increase in CK forward rate constant in the visual cortex. The novel imaging modalities, CKI and fCKI, have the potential to offer insights into brain bioenergetics both at rest and during activity, in both healthy and pathological conditions. Significance Creatine kinase (CK) is a key enzyme in brain bioenergetics, with its activity altered in various neurological disorders. This study introduces a CK Imaging (CKI) method to map CK forward rate constants across the entire brain in vivo. Furthermore, functional CKI extends this approach by capturing CK activation during functional tasks, revealing increased CK activity in the visual cortex during visual stimulation. The introduced methods could offer insights into brain energetics under both healthy and pathological conditions.