12期

最新文章

• Live-cell 3D-SIM of Rift Valley fever virus NSs filaments reveals a polygon web architecture AI翻译
  作者：Dunlop, James I.; Thomason, Peter A.; Carlin, Leo M.; Davis, Benjamin G.; Carter, Stephen D.
  作者单位：University of Glasgow; UK Research & Innovation (UKRI); Medical Research Council UK (MRC); University of Glasgow; Rosalind Franklin Institute; University of Oxford
  摘要：A defining feature of Rift Valley fever virus (RVFV) is the incorporation of the NSs protein into large filamentous assemblies inside infected nuclei [R. Swanepoel, N. K. Blackburn, J. Gen. Virol. 34, 557-561 (1977).], as judged from fixed specimens. To gain insight into the 3D structure of NSs filaments within live-cell nuclei, we used genetic-code expansion (GCE) to incorporate trans-cyclooct-2-en-L-lysine into the protein. This enabled site-specific fluorescent labeling with tetrazine dyes ...
• Orthogonal disentanglement of single-cell multi-omics reveals private and shared drivers of tissue development and pathogenesis AI翻译
  作者：Fan, Yi; Su, Yanchi; Hao, Gaoyang; Wang, Fuzhou; Chen, Xingjian; Wong, Ka-Chun; Li, Xiangtao
  作者单位：Jilin University; City University of Hong Kong; National Cancer Center - Japan; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Massachusetts General Hospital
  摘要：Characterizing gene expression and regulatory dynamics underlying both normal tissue function and disease progression requires an integrative analysis of single-cell multi-omics data. However, the asynchrony of gene regulation and the snapshot of single-cell multi-omics data give rise to private signals unique to each omics layer and shared signals reflecting cross-modality coordination. Here, we present Omics Separation Modeling using Domain Adaptation (OmiDos), a flexible annotation-free dee...
• Dislodging the drift barrier: Why do mutation rates vary? AI翻译
  作者：Schaack, Sarah
  作者单位：Reed College - Portland
• Quantitative system drift AI翻译
  作者：Veller, Carl; Muralidhar, Pavitra
  作者单位：University of Chicago; Northwestern University; University of Chicago
  摘要：We consider a biological system composed of multiple genetically variable components, the combined result of which is a quantitative trait under stabilizing selection for an optimal value. We show mathematically that, while the mean value of the system is ultimately constrained to remain near its optimum, the mean contributions of individual components are free to drift far from their initial values. Each component's drift, though qualitatively identical to neutral drift, is slower by a factor...
• Calsyntenin-3 suppresses inflammation via inhibition of TLR N-glycosylation and membrane localization AI翻译
  作者：Duan, Jiaqi; Zhang, Shikun; Wang, Bingjing; Liu, Yin; Liu, Lun; Liu, Yang; Wu, Jiacheng; Cao, Xuetao
  作者单位：Chinese Academy of Medical Sciences - Peking Union Medical College; Institute of Basic Medical Sciences - CAMS; Peking Union Medical College; Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College; Nankai University
  摘要：Excessive innate immune activation drives uncontrolled inflammation and multiple inflammatory diseases. Proper N-glycosylation of membrane-associated Toll-like receptor 4 (TLR4) is essential for its trafficking to the cell membrane and subsequent innate activation, yet the mechanisms regulating this process remain poorly understood. Through a genome-wide CRISPR screening, we identify calsyntenin-3 (CLSTN3) as a potent suppressor of TLR4-triggered inflammation in macrophages. Mechanistically, C...
• The radical SAM enzyme EpeE exhibits distinct site reactivity during the biosynthesis of the RiPP natural product epipeptide AI翻译
  作者：Walls, William G.; Yang, Hao; Ho, Madeline B.; Broderick, William E.; Hoffman, Brian M.; Broderick, Joan B.
  作者单位：Montana State University System; Montana State University Bozeman; Northwestern University
  摘要：Radical S-adenosyl-l-methionine (SAM) enzymes figure prominently in the formation of ribosomally synthesized and posttranslationally modified peptides (RiPPs), where they catalyze peptide modifications including epimerization, thioether crosslink formation, and peptide backbone splicing. Here, we use rapid freeze-quench trapping together with electron paramagnetic resonance and electron-nuclear double resonance techniques to probe the mechanistic steps of the two epimerization reactions cataly...
• Phosphate scarcity governs methane production in the global open ocean AI翻译
  作者：Wang, Shengyu; Xu, Hairong; Weber, Thomas S.
  作者单位：University of Rochester
  摘要：The observed supersaturation of methane (CH4) in open-ocean surface waters implies widespread CH4 production within the well-oxygenated mixed layer, driving emissions of this potent greenhouse gas to the atmosphere. The dominant CH4 production pathway that explains this phenomenon remains poorly understood, although candidates include production during photosynthesis, zooplankton metabolism, and dissolved organic matter cycling. Here, we construct a data-assimilating model of the open-ocean CH...
• Biosphere expansion drives Earth's secular oxygenation while tectonics modulate oxygen variability revealed by machine learning AI翻译
  作者：Zhang, Zhen- Jie; Tang, Dong- Jie; Cheng, Qiu- Ming
  作者单位：China University of Geosciences; China University of Geosciences
  摘要：The rise of atmospheric oxygen fundamentally transformed Earth's surface environment and enabled the evolution of complex life. However, the processes driving longterm oxygen fluctuations remain poorly resolved, partly from limited proxy resolution and temporal coverage. Trace element (TE) concentrations in sedimentary pyrite offer a robust archive of redox conditions in ancient oceans and their linkage to atmospheric oxygen levels. Here we integrate high-resolution geochemical data from pyrit...
• Reply to Vankov et al.: Reasoning traces are linked to accuracy and capture key dimensions of problem complexity AI翻译
  作者：De Varda, Andrea Gregor; D'elia, Ferdinando Pio; Kean, Hope; Lampinen, Andrew; Fedorenko, Evelina
  作者单位：Massachusetts Institute of Technology (MIT); University of Copenhagen; Alphabet Inc.; DeepMind
• Cob-binding protein as an antigenic target in the pathogenesis of antiphosphatidylethanolamine autoantibodies AI翻译
  作者：Hou, Songwang; Lindholm, Paul F.; Thurman, Joshua M.; Lee, Jungwha; Ramsey-Goldman, Rosalind; Zhao, Ming
  作者单位：Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine; University of Colorado System; University of Colorado Anschutz; University of Colorado Denver; Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine
  摘要：Autoantibodies against phosphatidylethanolamine (PE), a major phospholipid in cell membranes, are associated with symptoms of thrombosis and obstetric complications. A growing body of evidence indicates the involvement of a cofactor for the reactivity of anti-PE (aPE) antibodies. The goals of this study were to identify the putative cofactor and investigate the pathogenic roles of aPE antibodies. Existing ELISA-based assays for detecting aPE antibodies showed that the bovine plasma is a known ...