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作者：Cohen, Jon
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作者：Merchant, Hugo; Rajendran, Vani
作者单位：Universidad Nacional Autonoma de Mexico
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作者：Delaveris, Corleone S.; Loudermilk, Rita P.; Pandey, Apurva; Remesh, Soumya G.; Peters-Clarke, Trenton M.; Ganjave, Snehal D.; Dougherty, William J. N.; Delavan, Henry M.; Wang, Chunyue; Ling, Jesse; Camara Serrano, Juan Antonio; Salangsang, Fernando; Ding, Chien-Kuang Cornelia; Greenland, Nancy; Chu, Carissa E.; Porten, Sima; Steri, Veronica; Chou, Jonathan; Evans, Michael J.; Leung, Kevin K.; Wells, James A.
作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; UCSF Medical Center; UCSF Helen Diller Family Comprehensive Cancer Center; University of California System; University of California San Francisco; UCSF Medical Center; UCSF Helen Diller Family Comprehensive Cancer Center; University of California System; University of California San Francisco; University of California System; University of California San Francisco
摘要：Overexpression of the proto-oncogene Src is common to a wide variety of cancers. In this work, we found that Src is noncanonically translocated and inverted onto the cell surface in cancer, both in vitro and in vivo. We identified autophagolysosomal exocytosis (ALE) as a secretory mechanism prominent in cancer cell lines. Src represents the prototypical example of a family of membrane-anchored proteins that are transported by this process. Furthermore, this extracellular membrane-associated Sr...
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作者：Howell, Evan
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作者：Zhao, Celina
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作者：Bedbrook, Claire N.; Nath, Ravi D.; Zhang, Libby; Linderman, Scott W.; Brunet, Anne; Deisseroth, Karl
作者单位：Stanford University; Stanford University; Stanford Medicine; Stanford University; Stanford University; Stanford University; Stanford University; Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; Stanford University; Howard Hughes Medical Institute
摘要：Mapping behavior of individual vertebrate animals across lifespan could provide an unprecedented view into the lifelong process of aging. We created a platform for high-resolution continuous behavioral tracking of the African killifish across natural lifespan from adolescence to death. We found that animals follow distinct individual aging trajectories. The behaviors of long-lived animals differed markedly from those of short-lived animals, even relatively early in life, and were linked to org...
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作者：Funk, Mathis; Spronken, Monique I.; Hutchinson, Roy M.; Arragain, Benoit; Juyoux, Pauline; Bestebroer, Theo M.; De Bruin, Anja C. M.; Gultyaev, Alexander P.; Fouchier, Ron A. M.; Cusack, Stephen; Te Velthuis, Aartjan J. W.; Richard, Mathilde
作者单位：Erasmus University Rotterdam; Erasmus University Rotterdam - Excl Erasmus MC; Erasmus MC; Centre National de la Recherche Scientifique (CNRS); European Molecular Biology Laboratory (EMBL); Communaute Universite Grenoble Alpes; CEA; Universite Grenoble Alpes (UGA); Leiden University - Excl LUMC; Leiden University; Princeton University; Institute of Science & Technology - Austria; Medical University of Innsbruck
摘要：Highly pathogenic avian influenza viruses (HPAIVs) derive from H5 and H7 low pathogenic avian influenza viruses (LPAIVs). Although insertion of a furin-cleavable multibasic cleavage site (MBCS) in the hemagglutinin gene was identified decades ago as the genetic basis for the LPAIV-to-HPAIV transition, the mechanisms underlying the occurrence of insertion are unknown. Here, we show that transient H5 RNA structures, predicted to trap the influenza virus polymerase on purine-rich sequences, drive...
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作者：Cavanagh, Peter E.; Xue, Andrew G.; Dai, Shizhong A.; Qiang, Albert; Matsui, Tsutomu; Ting, Alice Y.
作者单位：Stanford Medicine; Stanford University; Stanford University; Stanford Medicine; Stanford University; United States Department of Energy (DOE); Stanford University; SLAC National Accelerator Laboratory; Stanford University; Stanford University; Stanford University
摘要：Conformational biasing (CB) is a rapid and streamlined computational method that uses contrastive scoring by inverse folding models to predict protein variants biased toward desired conformational states. We successfully validated CB across seven diverse datasets, identifying variants of K-Ras, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, the beta 2 adrenergic receptor, and Src kinase with improved conformation-specific functions such as enhanced binding or e...
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作者：Perry, Nicholas T.; Bartie, Liam J.; Katrekar, Dhruva; Gonzalez, Gabriel A.; Durrant, Matthew G.; Pai, James J.; Fanton, Alison; Martins, Juliana Q.; Hiraizumi, Masahiro; Ricci-Tam, Chiara; Nishimasu, Hiroshi; Konermann, Silvana; Hsu, Patrick D.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of Tokyo; University of Tokyo; Stanford Medicine; Stanford University; University of California System; University of California Berkeley
摘要：Bridge recombinases are naturally occurring RNA-guided DNA recombinases that we previously demonstrated can programmably insert, excise, and invert DNA in vitro and in Escherichia coli. In this study, we report the discovery and engineering of the bridge recombinase ortholog ISCro4 for universal rearrangements of the human genome. We defined strategies for the optimal application of bridge systems, leveraging mechanistic insights to improve their targeting specificity. Through rational enginee...
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作者：Neel, Benjamin G.; Maitra, Anirban
