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作者：Chao, Yu-Kai; Wu, Michelle; Gong, Qiyu; Chen, Fei
作者单位：Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard University
摘要：Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and stores transcriptomes within living mammalian cells for future readout. TimeVault leverages engineered vault particles that capture messenger RNA through polyadenosine [poly (A)]-binding protein. We demonstrate that the transcriptome stored by TimeVaults is stable in living cells for more than 7 da...
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作者：Faherty, Jackie
作者单位：American Museum of Natural History (AMNH)
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作者：Ling, Yick Hin; Liang, Chloe; Wang, Sixiang; Wu, Carl
作者单位：Johns Hopkins University; Johns Hopkins University; Johns Hopkins Medicine
摘要：Eukaryotic gene expression is orchestrated by RNA polymerases (RNAPI, II, and III) and associated factors, yet their real-time dynamics remain obscure. Using single-molecule tracking in living yeast, we quantified the kinetics of 58 proteins encompassing three RNAP machineries. RNAPI and RNAPIII preinitiation complexes (PICs) engage in long-lived chromatin interactions, contrasting with transient RNAPII PICs. We further report kinetics of RNAPII-associated factors for elongation, histone modif...
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作者：Wade, Lizzie
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作者：Anstett, Daniel N.; Anstett, Julia; Sheth, Seema N.; Moxley, Dylan R.; Branch, Haley A.; Jahani, Mojtaba; Huang, Kaichi; Todesco, Marco; Jordan, Rebecca; Lazaro-Guevara, Jose Miguel; Rieseberg, Loren H.; Angert, Amy L.
作者单位：University of British Columbia; University of British Columbia; Cornell University; Cornell University; Michigan State University; Michigan State University; Michigan State University; University of British Columbia; University of British Columbia; North Carolina State University; University of British Columbia; Yale University; Sun Yat Sen University; University of British Columbia; University of British Columbia Okanagan; Commonwealth Scientific & Industrial Research Organisation (CSIRO); CSIRO Environment; McGill University; McGill University; University of British Columbia
摘要：Populations that are declining as a result of climate change may need to evolve to persist. Although evolutionary rescue has been demonstrated in theory and in the laboratory, its relevance to natural populations facing climate change remains unknown. Here we link rapid evolution and population dynamics in scarlet monkeyflower, Mimulus cardinalis, during exceptional drought. We leverage whole-genome sequencing across 55 populations to identify climate-associated loci. Simultaneously we track d...
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作者：Dogra-Freitag, Aashima
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作者：Ervijn, Lien; Sanders, Niek N.
作者单位：Ghent University; Ghent University
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作者：Mervis, Jeffrey
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作者：Robison, Tanner A.; Mao, Yuwei; Oh, Zhen Guo; Ang, Warren S. L.; Loh, Dan Hong; Hsieh, Yu-Heng; Ceminsky, Maddie; Atkinson, Nicky; Lafferty, Declan; Xu, Xia; Gunn, Laura H.; McCormick, Alistair J.; Li, Fay-Wei
作者单位：Cornell University; Boyce Thompson Institute for Plant Research; Cornell University; University of Edinburgh; University of Edinburgh; Cornell University
摘要：In many algae, photosynthesis is boosted by biophysical CO2-concentrating mechanisms, which pack the CO2-fixing enzyme Rubisco into liquid-like organelles called pyrenoids. Engineering C3 crops with algal pyrenoids could increase yields, but progress is hampered by the deep evolutionary divide between crops and algae. Here, we show that hornworts, the only land plants with pyrenoids, have an unusual Rubisco small subunit, RbcS-STAR, the C-terminal coiled-coil domain of which oligomerizes to me...
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作者：Zukowski, Alan; Stepien, Martyna
作者单位：University of Wroclaw
