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作者：Tian, Rongzhen; Rehm, Fabian B. H.; Czernecki, Dariusz; Gu, Yangqi; Zurcher, Jerome F.; Liu, Kim C.; Chin, Jason W.
作者单位：MRC Laboratory Molecular Biology
摘要：The evolution of new function in living organisms is slow and fundamentally limited by their critical mutation rate. Here, we established a stable orthogonal replication system in Escherichia coli. The orthogonal replicon can carry diverse cargos of at least 16.5 kilobases and is not copied by host polymerases but is selectively copied by an orthogonal DNA polymerase (O-DNAP), which does not copy the genome. We designed mutant O-DNAPs that selectively increase the mutation rate of the orthogon...
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作者：Baker, David; Church, George
作者单位：University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; Harvard University; Harvard Medical School
摘要：The power and accuracy of computational protein design have been increasing rapidly with the incorporation of artificial intelligence (AI) approaches. This promises to transform biotechnology, enabling advances across sustainability and medicine. DNA synthesis plays a critical role in materializing designed proteins. However, as with all major revolutionary changes, this technology is vulnerable to misuse and the production of dangerous biological agents. To enable the full benefits of this re...
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作者：Lengnick-Hall, Rebecca
作者单位：Washington University (WUSTL)
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作者：Servick, Kelly; Chakradhar, Shraddha
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作者：Zhang, Jinfeng; Lin, Ting; Wang, Ao; Wang, Xiaochao; He, Qingyu; Ye, Huan; Lu, Jingdi; Wang, Qing; Liang, Zhengguo; Jin, Feng; Chen, Shengru; Fan, Minghui; Guo, Er-Jia; Zhang, Qinghua; Gu, Lin; Luo, Zhenlin; Si, Liang; Wu, Wenbin; Wang, Lingfei
作者单位：Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; Institute of Physics, CAS; Northwest University Xi'an; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Tsinghua University; Tsinghua University; Technische Universitat Wien; Anhui University; Collaborative Innovation Center of Advanced Microstructures (CICAM); Nanjing University
摘要：Identifying a suitable water-soluble sacrificial layer is crucial to fabricating large-scale freestanding oxide membranes, which offer attractive functionalities and integrations with advanced semiconductor technologies. Here, we introduce a water-soluble sacrificial layer, super-tetragonal Sr4Al2O7 (SAO(T)). The low-symmetric crystal structure enables a superior capability to sustain epitaxial strain, allowing for broad tunability in lattice constants. The resultant structural coherency and d...
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作者：[Anonymous]
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作者：Dai, Dai; Gu, Shuangshuang; Han, Xiaxia; Ding, Huihua; Jiang, Yang; Zhang, Xiaoou; Yao, Chao; Hong, Soonmin; Zhang, Jinsong; Shen, Yiwei; Hou, Guojun; Qu, Bo; Zhou, Haibo; Qin, Yuting; He, Yuke; Ma, Jianyang; Yin, Zhihua; Ye, Zhizhong; Qian, Jie; Jiang, Qian; Wu, Lihua; Guo, Qiang; Chen, Sheng; Huang, Chuanxin; Kottyan, Leah C.; Weirauch, Matthew T.; Vinuesa, Carola G.; Shen, Nan
作者单位：Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Tongji University; Chinese Academy of Sciences; Shanghai Institute of Nutrition & Health, CAS; University of Chinese Academy of Sciences, CAS; Capital Institute of Pediatrics (CIP); Shanghai Jiao Tong University; Chinese Academy of Sciences; University System of Ohio; University of Cincinnati; Cincinnati Children's Hospital Medical Center; University System of Ohio; University of Cincinnati; Francis Crick Institute
摘要：Age-associated B cells (ABCs) accumulate during infection, aging, and autoimmunity, contributing to lupus pathogenesis. In this study, we screened for transcription factors driving ABC formation and found that zinc finger E-box binding homeobox 2 (ZEB2) is required for human and mouse ABC differentiation in vitro. ABCs are reduced in ZEB2 haploinsufficient individuals and in mice lacking Zeb2 in B cells. In mice with toll-like receptor 7 (TLR7)-driven lupus, ZEB2 is essential for ABC formation...
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作者：Toma, Tiago S. P.; Oliveira, Hernani F. M.; Overbeck, Gerhard E.; Grelle, Carlos E. V.; Roque, Fabio O.; Negreiros, Daniel; Rodrigues, Domingos J.; Guimaraes, Aretha F.; Streit, Helena; Dechoum, Michele S.; Fonseca, Nathan C.; Rocha, Taina C.; Pereira, Cassio C.; Garda, Adrian A.; Bergallo, Helena G.; Domingos, Fabricius M. C. B.; Fernandes, Geraldo W.
作者单位：Universidade Federal de Minas Gerais; Universidade Federal do Parana; Universidade Federal do Rio Grande do Sul; Universidade Federal do Rio de Janeiro; Universidade Federal de Mato Grosso do Sul; Universidade Federal de Mato Grosso; Institute Nacional de Pesquisas da Amazonia; Universidade Federal de Santa Catarina (UFSC); Universidade Federal Rural de Pernambuco (UFRPE); Universidade Federal do Rio Grande do Norte; Universidade do Estado do Rio de Janeiro
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作者：Greenhill, Simon; Druckenmiller, Hannah; Wang, Sherrie; Keiser, David A.; Girotto, Manuela; Moore, Jason K.; Yamaguchi, Nobuhiro; Todeschini, Alberto; Shapiro, Joseph S.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; Resources for the Future; California Institute of Technology; Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); University of Massachusetts System; University of Massachusetts Amherst; Iowa State University; National Bureau of Economic Research; University of California System; University of California Berkeley; United States Department of Energy (DOE); University of California System; University of California Berkeley; University of California System; University of California Berkeley
摘要：We assess which waters the Clean Water Act protects and how Supreme Court and White House rules change this regulation. We train a deep learning model using aerial imagery and geophysical data to predict 150,000 jurisdictional determinations from the Army Corps of Engineers, each deciding regulation for one water resource. Under a 2006 Supreme Court ruling, the Clean Water Act protects two-thirds of US streams and more than half of wetlands; under a 2020 White House rule, it protects less than...
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作者：Kunzig, Robert
摘要：Pumped storage hydropower plants can bank energy for times when wind and solar power fall short
