作者：Fudenberg, Geoffrey; Ramani, Vijay
作者单位：University of Southern California; University of California System; University of California San Francisco
作者：Lewandowsky, Stephan
作者单位：University of Bristol; University of Potsdam
作者：Zavattieri, Pablo D.
作者单位：Purdue University System; Purdue University
作者：Oberstaller, Jenna; Xu, Shulin; Naskar, Deboki; Zhang, Min; Wang, Chengqi; Gibbons, Justin; Pires, Camilla Valente; Mayho, Matthew; Otto, Thomas D.; Rayner, Julian C.; Adams, John H.
作者单位：State University System of Florida; University of South Florida; University of Cambridge; Wellcome Trust Sanger Institute; University of Glasgow; Universite de Montpellier; Centre National de la Recherche Scientifique (CNRS); Universite de Montpellier; Institut National de la Sante et de la Recherche Medicale (Inserm); Mahidol University
摘要：Malaria parasites are highly divergent from model eukaryotes. Large-scale genome engineering methods effective in model organisms are frequently inapplicable, and systematic studies of gene function are few. We generated more than 175,000 transposon insertions in the Plasmodium knowlesi genome, averaging an insertion every 138 base pairs, and used this supersaturation mutagenesis to score essentiality for 98% of genes. The density of mutations allowed mapping of putative essential domains with...
