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作者：Schmidt, Ralf; Ward, Carl C.; Dajani, Rama; Armour-Garb, Zev; Ota, Mineto; Allain, Vincent; Hernandez, Rosmely; Layeghi, Madeline; Xing, Galen; Goudy, Laine; Dorovskyi, Dmytro; Wang, Charlotte; Chen, Yan Yi; Ye, Chun Jimmie; Shy, Brian R.; Gilbert, Luke A.; Eyquem, Justin; Pritchard, Jonathan K.; Dodgson, Stacie E.; Marson, Alexander
作者单位：Medical University of Vienna; Stanford University; University of California System; University of California San Francisco; Institut National de la Sante et de la Recherche Medicale (Inserm); Assistance Publique Hopitaux Paris (APHP); Universite Paris Cite; Hopital Universitaire Saint-Louis - APHP; University of California System; University of California Berkeley; University of California System; University of California San Francisco; UCSF Medical Center; UCSF Helen Diller Family Comprehensive Cancer Center; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; Stanford University; University of California System; University of California San Francisco; University of California System; University of California Berkeley
摘要：CRISPR-enabled screening is a powerful tool for the discovery of genes that control T cell function and has nominated candidate targets for immunotherapies1-6. However, new approaches are required to probe specific nucleotide sequences within key genes. Systematic mutagenesis in primary human T cells could reveal alleles that tune specific phenotypes. DNA base editors are powerful tools for introducing targeted mutations with high efficiency7,8. Here we develop a large-scale base-editing mutag...
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作者：Sepp, Mari; Leiss, Kevin; Murat, Florent; Okonechnikov, Konstantin; Joshi, Piyush; Leushkin, Evgeny; Spaenig, Lisa; Mbengue, Noe; Schneider, Celine; Schmidt, Julia; Trost, Nils; Schauer, Maria; Khaitovich, Philipp; Lisgo, Steven; Palkovits, Miklos; Giere, Peter; Kutscher, Lena M.; Anders, Simon; Cardoso-Moreira, Margarida; Sarropoulos, Ioannis; Pfister, Stefan M.; Kaessmann, Henrik
作者单位：Ruprecht Karls University Heidelberg; Helmholtz Association; German Cancer Research Center (DKFZ); INRAE; Helmholtz Association; German Cancer Research Center (DKFZ); Helmholtz Association; German Cancer Research Center (DKFZ); Helmholtz Association; German Cancer Research Center (DKFZ); Leibniz Institut fur Evolutions und Biodiversitatsforschung; Chongqing Medical University; Newcastle University - UK; Semmelweis University; Ruprecht Karls University Heidelberg; Francis Crick Institute; Ruprecht Karls University Heidelberg; Helmholtz Association; German Cancer Research Center (DKFZ); Ruprecht Karls University Heidelberg; National Center for Tumor Diseases; Wellcome Trust Sanger Institute
摘要：The expansion of the neocortex, a hallmark of mammalian evolution1,2, was accompanied by an increase in cerebellar neuron numbers3. However, little is known about the evolution of the cellular programmes underlying the development of the cerebellum in mammals. In this study we generated single-nucleus RNA-sequencing data for around 400,000 cells to trace the development of the cerebellum from early neurogenesis to adulthood in human, mouse and the marsupial opossum. We established a consensus ...
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作者：Cao, Xiao-Yu
摘要：Mice that work out regularly have higher levels of a molecule that promotes muscle regeneration than sedentary mice do.
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作者：Couture, Victor
作者单位：University of British Columbia
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作者：Webster, Shanice S.; Guerinot, Mary Lou
作者单位：Duke University; Duke University; Howard Hughes Medical Institute; Dartmouth College
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作者：Opacak, Nikola; Kazakov, Dmitry; Columbo, Lorenzo L.; Beiser, Maximilian; Letsou, Theodore P.; Pilat, Florian; Brambilla, Massimo; Prati, Franco; Piccardo, Marco; Capasso, Federico; Schwarz, Benedikt
作者单位：Technische Universitat Wien; Harvard University; Massachusetts Institute of Technology (MIT); Politecnico di Bari; Politecnico di Bari; Consiglio Nazionale delle Ricerche (CNR); Istituto di Fotonica e Nanotecnologie (IFN-CNR); University of Insubria; Universidade de Lisboa
摘要：Optical frequency-comb sources, which emit perfectly periodic and coherent waveforms of light1, have recently rapidly progressed towards chip-scale integrated solutions. Among them, two classes are particularly significant-semiconductor Fabry-Perot lasers2-6 and passive ring Kerr microresonators7-9. Here we merge the two technologies in a ring semiconductor laser10,11 and demonstrate a paradigm for the formation of free-running solitons, called Nozaki-Bekki solitons. These dissipative waveform...
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作者：Baca, Christian F.; Yu, You; Rostol, Jakob T.; Majumder, Puja; Patel, Dinshaw J.; Marraffini, Luciano A.
作者单位：Rockefeller University; Cornell University; Weill Cornell Medicine; Rockefeller University; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Rockefeller University; Howard Hughes Medical Institute; Imperial College London
摘要：Prokaryotic type III CRISPR-Cas systems provide immunity against viruses and plasmids using CRISPR-associated Rossman fold (CARF) protein effectors1-5. Recognition of transcripts of these invaders with sequences that are complementary to CRISPR RNA guides leads to the production of cyclic oligoadenylate second messengers, which bind CARF domains and trigger the activity of an effector domain6,7. Whereas most effectors degrade host and invader nucleic acids, some are predicted to contain transm...
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作者：Young, Dylan J.; Chu, Anjun; Song, Eric Yilun; Barberena, Diego; Wellnitz, David; Niu, Zhijing; Schaefer, Vera M.; Lewis-Swan, Robert J.; Rey, Ana Maria; Thompson, James K.
作者单位：National Institute of Standards & Technology (NIST) - USA; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder; Max Planck Society; University of Oklahoma System; University of Oklahoma - Norman; University of Oklahoma System; University of Oklahoma - Norman
摘要：In conventional Bardeen-Cooper-Schrieffer superconductors1, electrons with opposite momenta bind into Cooper pairs due to an attractive interaction mediated by phonons in the material. Although superconductivity naturally emerges at thermal equilibrium, it can also emerge out of equilibrium when the system parameters are abruptly changed2-8. The resulting out-of-equilibrium phases are predicted to occur in real materials and ultracold fermionic atoms, but not all have yet been directly observe...
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作者：Hughes, Alice E.; Freathy, Rachel M.
作者单位：University of Exeter
摘要：Maternal sensitivity to a hormone produced by the fetus might underlie the risk of severe nausea and vomiting in human pregnancy - a finding that could open up strategies for the treatment of this debilitating condition.
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作者：Naddaf, Miryam
