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作者：Burn, Garth Lawrence; Raisch, Tobias; Tacke, Sebastian; Winkler, Moritz; Prumbaum, Daniel; Thee, Stephanie; Gimber, Niclas; Raunser, Stefan; Zychlinsky, Arturo
作者单位：Max Planck Society; Max Planck Society; Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; Free University of Berlin; Humboldt University of Berlin; Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; Berlin Institute of Health; Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin
摘要：Neutrophils, the most abundant and biotoxic immune cells, extrude nuclear DNA into the extracellular space to maintain homeostasis. Termed neutrophil extracellular traps (NETs), these protein-modified and decondensed extracellular DNA scaffolds control infection and are involved in coagulation, autoimmunity and cancer1,2. Here we show how myeloperoxidase (MPO), a highly expressed neutrophil protein, disassembles nucleosomes, thereby facilitating NET formation, yet also binds stably to NETs ext...
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作者：Nordling, Linda
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作者：Orta-Martinez, Marti; Munoa, Gorka; Llavero-Pasquina, Marcel; Rius-Taberner, Guillem
作者单位：University of Barcelona; ISGlobal
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作者：Castelvecchi, Davide
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作者：Rau, Gioia
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作者：Cui, Haiyang; Su, Yufeng; Dean, Tanner J.; Yu, Tianhao; Zhang, Zhengyi; Peng, Jian; Shukla, Diwakar; Zhao, Huimin
作者单位：University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; Nanjing Normal University
摘要：Enzymes are the molecular machines of life, and a key property that governs their function is substrate specificity-the ability of an enzyme to recognize and selectively act on particular substrates. This specificity originates from the three-dimensional (3D) structure of the enzyme active site and complicated transition state of the reaction1,2. Many enzymes can promiscuously catalyse reactions or act on substrates beyond those for which they were originally evolved1,3, 4-5. However, millions...
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作者：Cheng, Cong; Dai, Jian; Yan, Lulu; Wang, Chengliang
作者单位：Zhejiang University of Technology; East China Normal University
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作者：Tang, Shangming; Hariri, Sara; Bohn, Regina; McCarthy, John E.; Koo, Jennifer; Pourhosseinzadeh, Mohammad; Nguyen, Emerald; Liu, Natalie; Ma, Christopher; Lu, Hanyu; Lee, Monica; Hunter, Neil
作者单位：University of California System; Howard Hughes Medical Institute; University of California Davis; University of California System; University of California Davis; University of Virginia (UVA) Health System; University of Virginia; University of California System; University of California Davis
摘要：Chromosomal linkages formed through crossover recombination are essential for the accurate segregation of homologous chromosomes during meiosis1. The DNA events of recombination are linked to structural components of meiotic chromosomes2. Imperatively, the biased resolution of double Holliday junction (dHJ) intermediates into crossovers3,4 occurs within the synaptonemal complex (SC), the meiosis-specific structure that mediates end-to-end synapsis of homologues during the pachytene stage5,6. H...
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作者：Kelepouras, Konstantinos; Saggau, Julia; Bonasera, Debora; Kiefer, Christine; Locci, Federica; Rakhsh-Khorshid, Hassan; Grauvogel, Louisa; Varanda, Ana Beatriz; Peifer, Martin; Loricchio, Elena; Montinaro, Antonella; Croon, Marijana; Trifunovic, Aleksandra; Prencipe, Giusi; Insalaco, Antonella; De Benedetti, Fabrizio; Walczak, Henning; Liccardi, Gianmaria
作者单位：University of Cologne; University of Cologne; University of Cologne; University of Cologne; University of Cologne; Max Planck Society; University of Cologne; IRCCS Bambino Gesu; University of London; University College London; University of Cologne; University of Cologne
摘要：Conditional deletion of caspase-8 in mouse epidermal keratinocytes (Casp8(E-KO)) causes necroptosis-driven lethal dermatitis(1-7). Here we find that the loss of Casp8 leads to an accumulation of cytosolic DNA that is responsible for the activation of a cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING)-mediated transcriptional program. Genetic and biochemical evidence indicate that STING upregulates both Z-DNA-binding protein 1 (ZBP1) and mixed lineage kinase domain-like pse...
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作者：Faraway, Rupert; Costello Heaven, Neve; Digby, Holly; Kuret Hodnik, Klara; Rebselj, Jure; Wilkins, Oscar G.; Chakrabarti, Anob M.; Iosub, Ira A.; Vadnjal, Neza; Dore, Rhys; Knez, Lea; Ameres, Stefan L.; Plaschka, Clemens; Ule, Jernej
作者单位：Francis Crick Institute; University of London; King's College London; University of London; King's College London; Vienna Biocenter (VBC); Research Institute of Molecular Pathology (IMP); National Institute of Chemistry - Slovenia; University of London; University College London; University of London; University College London
摘要：The concentration of proteins containing intrinsically disordered regions must be tightly controlled to maintain cellular homeostasis1,2. However, mechanisms for collective control of these proteins, which tend to localize to membraneless condensates, are less understood than pathways mediated by membrane-bound organelles3,4. Here we report 'interstasis', a homeostatic mechanism in which increased concentration of proteins within RNA-protein condensates induces the sequestration of their own m...
