43期

最新文章

• HIF2α negatively regulates MYCN protein levels and promotes a low- risk noradrenergic phenotype in neuroblastoma AI翻译
  作者：Yuan, Juan; Maitra, Subhamita; Antoniou, Eirini; Zhu, Jiacheng; Li, Wenyu; Demirel, Ilknur Safak; Toskas, Kostantinos; Martinez, Iria Laura; Ozcimen, Lacin; Lindehell, Henrik; Muhr, Jonas; Stenman, Jakob; Kogner, Per; Bedoya-Reina, Oscar C.; Schlisio, Susanne; Holmberg, Johan
  作者单位：Karolinska Institutet; Umea University; Karolinska Institutet; Karolinska Institutet; Karolinska Institutet; Orebro University
  摘要：The role of HIF2 alpha, encoded by EPAS1, in neuroblastoma remains controversial. Here, we demonstrate that induction of high levels of HIF2 alpha in MYCN- amplified neuroblastoma cells results in a rapid and profound reduction of the oncoprotein MYCN. This is followed by an upregulation of genes characteristic of noradrenergic cells in the adrenal medulla. Additionally, upon induction of HIF2 alpha, the proliferation rate drops substantially, and cells develop elongated neurite- like protrusi...
• Reply to Auspurg: On the limits of justified model spaces AI翻译
  作者：Ganslmeier, Michael; Vlandas, Tim
  作者单位：University of Exeter; University of Oxford; University of Oxford
• Hierarchical self-assembly for high-yield addressable complexity at fixed conditions AI翻译
  作者：Holmes-Cerfon, Miranda; Wyart, Matthieu
  作者单位：University of British Columbia; Johns Hopkins University; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne
  摘要：There is evidence that the self-assembly of complex molecular systems often proceeds hierarchically, by first building subunits that later assemble in larger entities, in a process that can repeat multiple times. Yet, our understanding of this phenomenon and its performance is limited. Here, we introduce a simple model for hierarchical addressable self-assembly, where interactions between particles can be optimized to maximize the fraction of a well-formed target structure, or yield. We find t...
• A steady- state pool of calcium- dependent actin is maintained by Homer and controls epithelial mechanosensation AI翻译
  作者：Matsuzawa, Kenji; Suzuki, Makoto; Cho, Yuma; Fujinaga, Ryoya; Ikenouchi, Junichi
  作者单位：Kyushu University; Hiroshima University
  摘要：Epithelial cells are inherently contractile and in homeostasis, tissue integrity is maintained by balancing the uneven contractile forces in neighboring cells at the cell-cell interface. By contrast, epithelial cells can utilize an imbalance in contractile force to communicate various information to induce tissue-wide response as in wound healing. Contractility is generated and processed at the apical junctional complex (AJC) by the dynamic behavior of the actin cytoskeleton. Calcium signaling...
• Outrunning protein diffusion to the air-water interface in cryoEM AI翻译
  作者：Gusach, Anastasiia; Sader, Kasim; Russo, Christopher J.
  作者单位：MRC Laboratory Molecular Biology; AstraZeneca
  摘要：Here, we report a series of measurements indicating that it is physically possible to thin and vitrify a specimen for electron cryomicroscopy (cryoEM) faster than proteins diffuse to the air-water interface. We achieved this by spraying picoliter volume droplets at speeds of hundreds of meters per second into a thin layer of liquid ethane coating the surface of a precooled specimen support. The droplets simultaneously collapsed and froze in microseconds into the amorphous phase as they landed ...
• Genetic regulation of the estrogen receptor and inherited predisposition to breast cancer AI翻译
  作者：Pierce, Sarah B.; Kortbawi, Hannah; Gulsuner, Suleyman; Mandell, Jessica B.; Lee, Ming K.; Walsh, Tom; King, Mary - Claire
  作者单位：University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; University of California System; University of California San Francisco; University of California System; University of California San Francisco
  摘要：For many families severely affected with breast cancer, no inherited causal allele has been detected in any tumor suppressor gene. In an effort to understand the genetics underlying breast cancer in these families, we evaluated 136 such families for coinheritance of breast cancer with each of 79 common variants reported as high-confidence risk alleles for breast cancer by meta-analyses of genome-wide association studies. Simulations based on allele frequencies and family structures revealed on...
• On the scale of heterogeneity in composite electrodes of batteries AI翻译
  作者：Shrivastava, Aishwary; Sharma, Nikhil; He, Xiaomei; Liu, Yijin; Zhao, Kejie
  作者单位：Purdue University System; Purdue University; Purdue University in Indianapolis; University of Texas System; University of Texas Austin
  摘要：An electrode in cylindrical or pouch cell batteries contains millions of active particles embedded in a conductive network. Battery performance, such as voltage, capacity, and cyclic efficiency, is a collective response of the particle network. We use optical microscopy to measure the local, heterogeneous state of charge of individual particles upon charging and discharging. The optical reflectivity is proportional to Li composition in the ternary oxide LiNi LiNix LiNixMn LiNixMny LiNixMnyCo L...
• Amyloid precursor protein and C99 are subunits in human microglial Hv1 channels that enhance current and inflammatory mediator release AI翻译
  作者：Zhao, Ruiming; Sophanpanichkul, Punyanuch; Chadarevian, Jean Paul; Ding, Yiwen; Dai, Hui; Nayak, Maha; Davtyan, Hayk; Blurton-Jones, Mathew; Goldstein, Steve A. N.
  作者单位：University of California System; University of California Irvine; University of California System; University of California Irvine; University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：In Alzheimer's disease (AD), hyperactivated microglia produce inflammatory mediators that contribute to neuroinflammation and neuronal damage. Amyloid precursor protein (APP), a transmembrane protein expressed in many cell types, including neurons and microglia, plays a critical role in AD pathogenesis via its secretase-mediated processing to release the C-terminal 99-residue transmembrane fragment (C99) that is further cleaved to yield amyloid-beta peptides. Voltage-gated proton channels (Hv1...
• Beyond adoption: The persistence of conservation and climate- smart agricultural practices in the United States AI翻译
  作者：Ferraro, Paul J.; Bowman, Maria; Correia, Hannah E.; Gao, Jing; Larson, Kelsey R.; Messer, Kent D.; Paul, Laura A.; Pratt, Bryan; Prokopy, Linda S.
  作者单位：Johns Hopkins University; Johns Hopkins Bloomberg School of Public Health; Johns Hopkins University; United States Department of Agriculture (USDA); University of North Dakota Grand Forks; University of Delaware; University of Delaware; Montana State University System; Montana State University Bozeman; University of Delaware; University of Vermont
  摘要：Achieving sustainability goals requires that humans change their behavior not just once but persistently. Yet despite decades of research on the adoption of conservation and climate-smart agricultural practices, little is known about the extent to which these practices persist over time. One key reason is the lack of longitudinal, field-level data. Using ground-verified, longitudinal data on cover cropping across thousands of farm parcels in Indiana (USA), we find that persistence is low and c...
• Cytosolic proliferating cell nuclear antigen (PCNA) orchestrates neutrophil hyperactivation in COVID-19 AI翻译
  作者：Formiga, Rodrigo de Oliveira; Pesenti, Lucie; de la Heronniere, Francois Chable; Ladjemi, Maha Zohra; Stojkov, Darko; Yousefi, Shida; Frachet, Philippe; Krafft, Lisa; Tiberio, Laura; Bosisio, Daniela; Andrieu, Muriel; Many, Souganya; Karunanithy, Vaarany; Bailly, Karine; Dhote, Theo; Tasso, Giovanni Saraceni -; Castel, Manon; Rousseau, Christophe; Starick, Marick Rodrigues; Rocha, Edroaldo Lummertz Da; Lombardi, Emilia Puig; Almeida, Cicero Jose Luiz dos Ramos; Ramos, Anderson dos Santos; Cunha, Fernando Queiroz; Alves-Filho, Jose Carlos; Nobrega, Natalia Ribeiro Cabacinha; Goncalves, Matheus Rodrigues; Queiroz, Celso Martins; Batista, Viviane Lima; Teixeira, Mauro Martins; Granger, Vanessa; Chollet-Martin, Sylvie; De Chaisemartin, Luc; Mouthon, Luc; Hosmalin, Anne; Hurtado-Nedelec, Margarita; Martin, Clemence; Spiller, Fernando; Simon, Hans- Uwe; Tamassia, Nicolas; Cassatella, Marco Antonio; Pene, Frederic; Vogl, Thomas; Burgel, Pierre- Regis; Costa, Vivian Vasconcelos; Witko, Veronique
  作者单位：Universite Paris Cite; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); CNRS - National Institute for Biology (INSB); Universite Paris Cite; Institut National de la Sante et de la Recherche Medicale (Inserm); Universidade Federal de Santa Catarina (UFSC); University of Bern; Centre National de la Recherche Scientifique (CNRS); CEA; Communaute Universite Grenoble Alpes; CNRS - National Institute for Biology (INSB); Universite Grenoble Alpes (UGA); University of Munster; University of Brescia; Universidade Federal de Santa Catarina (UFSC); Institut National de la Sante et de la Recherche Medicale (Inserm); Universite Paris Cite; Universidade de Sao Paulo; Universidade Federal de Minas Gerais; Universidade Federal de Minas Gerais; Universidade Federal de Minas Gerais; Universite Paris Cite; Assistance Publique Hopitaux Paris (APHP); Hopital Universitaire Bichat-Claude Bernard - APHP; Assistance Publique Hopitaux Paris (APHP); Universite Paris Cite; Hopital Universitaire Cochin - APHP; Institut National de la Sante et de la Recherche Medicale (Inserm); Universite Paris Cite; Universite Paris Cite; Assistance Publique Hopitaux Paris (APHP); Hopital Universitaire Bichat-Claude Bernard - APHP; Assistance Publique Hopitaux Paris (APHP); Universite Paris Cite; Hopital Universitaire Cochin - APHP; University of Verona; Assistance Publique Hopitaux Paris (APHP); Universite Paris Cite; Hopital Universitaire Cochin - APHP
  摘要：Neutrophils are central mediators of the hyperinflammatory response in severe SARS-CoV-2 infection. We report elevated cytosolic levels of proliferating cell nuclear antigen (PCNA) in neutrophils from patients with severe and critical COVID-19, correlating with enhanced NADPH oxidase-dependent reactive oxygen species (ROS) generation and neutrophil extracellular trap (NET) formation. Using T2AA, a small-molecule inhibitor of the PCNA scaffold, we demonstrate potent suppression of NADPH oxidase...